Imaging vitreous

Sebag, J.

doi:10.1038/sj.eye.6700201

Cited by 64 publications

(47 citation statements)

References 28 publications

(27 reference statements)

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“…However, its exact role and mechanisms of action have not been determined. 12,13 Thus, the purpose of this study was to determine whether extracellular histones are released from dying retinas with RD, and whether they affect the subretinal microenvironment as do the DAMP molecules. We shall show that the concentration of histone H3, which is component of the core histones, in the vitreous of eyes with RD was higher than that in control eyes.…”

mentioning

confidence: 99%

Toxic effects of extracellular histones and their neutralization by vitreous in retinal detachment

Kawano

Ito

Yamada³

et al. 2014

Laboratory Investigation

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Histones are DNA-binding proteins and are involved in chromatin remodeling and regulation of gene expression. Histones can be released after tissue injuries, and the extracellular histones cause cellular damage and organ dysfunction. Regardless of their clinical significance, the role and relevance of histones in ocular diseases are unknown. We studied the role of histones in eyes with retinal detachment (RD). Vitreous samples were collected during vitrectomy, and the concentration of histone H3 was measured by enzyme-linked immunosorbent assay. The location of the histones and related molecules was examined in a rat RD model. The release of histones and their effects on rat retinal progenitor cells R28 and ARPE-19 were evaluated in vitro. In addition, the protective role of the vitreous body against histones was tested. The intravitreal concentration of histones was higher in eyes with RD (mean, 30.9±9.8 ng/ml) than in control eyes (below the limit of detection, Po0.05). In the rat RD model, histone H3 was observed on the outer side of the detached retina and was associated with photoreceptor death. Histone H3 was released from cultured R28 by oxidative stress. Histones at a concentration 10 mg/ml induced the production of interleukin-8 in ARPE-19 cells (2.5-fold increase, Po0.05) that was mediated through the ERK1/2-and p38 MAPK-dependent pathways and Toll-like receptor 4. Histones were toxic to cells at concentrations of Z20 mg/ml. Vitreous body or hyaluronan decreased toxicity of histones by inhibiting diffusion of histones. These results indicate that histones are released from retinas with RD and may modulate the subretinal microenvironment by functioning as damage-associated molecular pattern molecules, thereby inducing proinflammatory cytokines or cell toxicity. In addition, the important role of the vitreous body and hyaluronan in protecting the retina from these toxic effects is suggested.

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mentioning

confidence: 99%

Toxic effects of extracellular histones and their neutralization by vitreous in retinal detachment

Kawano

Ito

Yamada³

et al. 2014

Laboratory Investigation

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“…5 It is believed that the development of a tool for the adequate imaging of the vitreous humor will only be accomplished by combining the use of more than 1 technique; therefore, there is a rationale for the exploration of new methods. 5,6 DWI is an MR imaging technique that provides image contrast related to the random microscopic motion of water protons. Diffusion depends on a variety of biophysical properties in tissue, including cell organization, attenuation, microstructure, and microcirculation, all of which change with age in the vitreous humor.…”

mentioning

confidence: 99%

Diffusion Changes in the Vitreous Humor of the Eye during Aging

Meral¹,

Bilgili²

2011

AJNR Am J Neuroradiol

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“…However, the presence of PVD can never be definitively established because the level of resolution is not sufficient to reliably image the posterior vitreous cortex. 4 Surgeons may occasionally encounter unexpected, severe conditions because of extensive and broad VRAs. We hypothesized that a correlation between axial length and PVD status might be helpful in such circumstances.…”

Section: Discussionmentioning

confidence: 99%

“…Accurate and adequate diagnostic tools for evaluating the vitreous is not yet available, and therefore PVD is perhaps the least accurate diagnosis entertained by ophthalmologists on a daily basis. 4 On the other hand, axial length is easily measured with preoperative US biometry, regardless of media opacity. In the normal population, PVD is reported more frequently in moderate or high myopia than in emmetropia, and axial length is a significant factor in the development of PVD in high myopia.…”

Section: Introductionmentioning

confidence: 99%

Axial length and intraoperative posterior vitreous detachment as predictive factors for surgical outcomes of diabetic vitrectomy

Kim

et al. 2010

Eye

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Aims To evaluate the relationship of axial length (AXL), intraoperatively assessed posterior vitreous detachment (PVD) status, and surgical outcomes of diabetic vitrectomy. Methods Retrospective, consecutive case series. Clinical records were reviewed for 115 eyes (50 males, 65 females) with more than a 6-month follow-up who underwent diabetic vitrectomy from a single surgeon. Thirty-three eyes had vitreous haemorrhage, 37 had tractional retinal detachment (TRD) threatening the macula, 43 had TRD involving the macula, and two had neovascular glaucoma. AXL was measured preoperatively by ultrasonography, and PVD status was classified intraoperatively: broad vitreo-retinal adhesion as no PVD, PVD at the macular area with attachment at the disc as incomplete PVD, and complete PVD. Results Forty-four eyes had no PVD, 23 had incomplete PVD, and 48 had complete PVD. A majority of the no PVD group had macula off TRD (97.7%), whereas vitreous haemorrhage (68.7%) predominated in the complete PVD group. Longer AXLs were noted in the complete PVD group compared with the no PVD and incomplete PVD groups (ANOVA in three groups P ¼ 0.0001). Univariate analysis showed that AXL had an influence on anatomical success (P ¼ 0.02). Multiple logistic regression analysis yielded that PVD status is a significant predictor of the final best corrected visual acuity (BCVA)420/100, and BCVA420/40 (P ¼ 0.01, P ¼ 0.02). Conclusions Intraoperatively assessed PVD status is a prognostic factor for functional outcomes of diabetic vitrectomy. Shorter AXL was associated with lesser PVD. In eyes with a lack of PVD, careful timing and decision of surgery are mandatory.

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Imaging vitreous

Cited by 64 publications

References 28 publications

Toxic effects of extracellular histones and their neutralization by vitreous in retinal detachment

Toxic effects of extracellular histones and their neutralization by vitreous in retinal detachment

Diffusion Changes in the Vitreous Humor of the Eye during Aging

Axial length and intraoperative posterior vitreous detachment as predictive factors for surgical outcomes of diabetic vitrectomy

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