Imaging the vasculature of a beating heart by dynamic speckle: the challenge of a quasiperiodic motion

Abstract

. The spatial and temporal evolution of the field backscattered by a beating heart while illuminated with a coherent light reveals its macro- and microvascularization in real time. To perform these vascularization images, we use a recently published method of laser speckle imaging, based on the selective detection of spatially depolarized speckle field that is mainly generated by multiple scattering. We consider the calculation of the speckle contrast, by a spatial or temporal estimation. We show that the signal-to-noise ratio of the observed vascular structure can be noticeably increased by a postprocessing method implying the calculation of a motion field that allows the selection of similar frames extracted from different heartbeat periods. This later optimization reveals vascular microstructures with a spatial resolution of the order of .

Wide dynamic range measurement of blood flow in vivo using laser speckle contrast imaging

“…These results provide significant guidance for future biomedical studies of high spatio-temporal resolution imaging and the spatio-temporal evolution of myocardial perfusion. In future work, we intend to address the motion artifacts caused by cardiac quasi periodic motion and respiration, as well as to detect the location of the stenosis, 33,34 which is a critical step in quantitatively monitoring the spatio-temporal evolution of myocardial perfusion in the beating heart in vivo before and after coronary artery bypass grafting.…”

Monitoring coronary blood flow by laser speckle contrast imaging after myocardial ischaemia reperfusion injury in adult and aged mice

“…Plyer and colleagues applied it to the ex vivo perfused healthy porcine heart but primarily used it to observe coronary vasculature rather than changes in perfusion per se . Moreover, complicated mathematical post-processing, based on estimating displacement between acquired frames, was needed to enhance the signal-to-noise ratio of the LSCI signal ( 15 ). Liu and colleagues applied dynamic light scattering combined with LSCI to the rabbit heart.…”