Imaging the cell wall of living single yeast cells using surface-enhanced Raman spectroscopy

Sujith, A.; Itoh, Tamitake; Abe, Hiroko; Yoshida, Kenichi; Kiran, Manikantan Syamala; Biju, Vasudevanpillai; Ishikawa, Misturu

doi:10.1007/s00216-009-2883-9

Cited by 64 publications

(32 citation statements)

References 27 publications

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“…The nanoparticles aggregated and deposited on the cell wall and enabled SERS detection. 98 Similar detection performed on bacterial cells coated with Au and Ag nanoparticles has been reported by Kahraman and co-workers (Fig. 4a).…”

Section: Biomedical Applications Of Serssupporting

confidence: 88%

Bio-imaging, detection and analysis by using nanostructures as SERS substrates

2011

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Surface-enhanced Raman scattering (SERS) is a phenomenon that occurs on nanoscale-roughed metallic surface. The magnitude of the Raman scattering signal can be greatly enhanced when the scatterer is placed in the very close vicinity of the surface, which enables this phenomenon to be a highly sensitive analytical technique. SERS inherits the general strongpoint of conventional Raman spectroscopy and overcomes the inherently small cross section problem of a Raman scattering. It is a sensitive and nondestructive spectroscopic method for biological samples, and can be exploited either for the delivery of molecular structural information or for the detection of trace levels of analytes. Therefore, SERS has long been regarded as a powerful tool in biomedical research. Metallic nanostructure plays a key role in all the biomedical applications of SERS because the enhanced Raman signal can only be obtained on the surface of a finely divided substrate. This review focuses on progress made in the use of SERS as an analytical technique in bio-imaging, analysis and detection. Recent progress in the fabrication of SERS active nanostructures is also highlighted.

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Section: Biomedical Applications Of Serssupporting

confidence: 88%

Bio-imaging, detection and analysis by using nanostructures as SERS substrates

2011

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“…Then the cell wall of living yeast cells could be detected by use of a Raman microspectroscopy. [72] Similar work has been reported by Kahraman coli and Staphylococcus cohnii bacteria cells with gold and silver nanoparticles for the acquisition of SERS spectra (Fig. 7).…”

Section: Label-free Sers In Cell Analysissupporting

confidence: 59%

Application of surface‐enhanced Raman scattering in cell analysis

Xie

Shen

et al. 2011

J Raman Spectroscopy

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In surface-enhanced Raman scattering (SERS), the scattered intensity is drastically increased due to a resonant interaction with surface plasmons of coin metals. SERS is a nondestructive spectroscopic method applied also to biomedical samples. It inherits the advantages of normal Raman spectroscopy and at the same time overcomes the inherent low sensitivity problem. These properties endow SERS with exciting opportunities to be a successful analytical tool for cell analysis. SERS can be used to detect only molecules located on or close to the metallic nanostructures which can support surface plasmon resonances for the enhancement of the Raman signals. Therefore, these metallic nanostructures play a key role in the application of SERS in cell analysis. By incorporating the SERS substrates into the biosamples, molecular structural probing and cellular imaging become possible. In the past decade, analysts worldwide have developed many schemes to study the chemical changes and component distribution in cells by using SERS. In this paper, the application of SERS in cell analysis is reviewed.

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“…Also, if an analyte is attached to, or is perhaps microscopically close to, a suitably roughened surface (substrate), vibrational mode coupling can also be enhanced. This technique is called surface-enhanced Raman scattering (SERS) (Sengupta et al 2006;Sujith et al 2009;Premasiri et al 2005). SERS substrates consist of either silver or gold nanomaterials-either colloidal or extended surfaces with nanoscale morphologies, both of which are appropriate for use with biological samples, making it possible to increase the Raman intensity by perhaps as much as 10 15 -fold (Chu et al 2008).…”

Section: Introduction To Infrared and Raman Spectroscopic Properties mentioning

confidence: 99%

Application of Mid-infrared and Raman Spectroscopy to the Study of Bacteria

Al-Qadiri

Lin

et al. 2011

Food Bioprocess Technol

202

120

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Infrared spectroscopy and Raman spectroscopy provide complementary technologies for rapid and precise detection of microorganisms and are emerging methods in food analysis. It is possible to use either of these techniques to differentiate and quantify microorganisms in relatively simple matrices such as liquid media and simple solutions with determinations taking less than an hour. Vibrational spectroscopy, unlike other techniques used in microbiology, is a relatively simple method for studying structural changes occurring within a microbial cell following environmental stress and applications of food processing treatments. Vibrational spectroscopy provides a wide range of biochemical properties about bacteria in a single spectrum, most importantly characteristics of the cell membrane. These techniques are especially useful for studying properties of bacterial biofilms on contact surfaces, the presence and viability of bacterial vegetative cells and spores, the type and degree of bacterial injury, and assessment of antibiotic susceptibility. Future trends in food analysis will involve combining vibrational spectroscopy with microscopy, mass spectroscopy, or DNA-based methods to comprehensively study bacterial stress. Further advances in selectivity, sensitivity, and improved chemometric methods, along with reduction in the cost of instrumentation, may lead to the development of fieldready and real-time analytical systems.

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Imaging the cell wall of living single yeast cells using surface-enhanced Raman spectroscopy

Cited by 64 publications

References 27 publications

Bio-imaging, detection and analysis by using nanostructures as SERS substrates

Bio-imaging, detection and analysis by using nanostructures as SERS substrates

Application of surface‐enhanced Raman scattering in cell analysis

Application of Mid-infrared and Raman Spectroscopy to the Study of Bacteria

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