Imaging Polyphenolic Compounds in Plant Tissues

Otegui, Marisa S.

doi:10.1002/9781119545958.ch11

Cited by 5 publications

(3 citation statements)

References 75 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Unfortunately, fluorescent labelling of procyanidins by dansylation did not reliably allow the detection of the main binding site of the molecules. However, the unexpected observation that the intrinsic fluorescence of the cuticle-bound procyanidins 62 within the extract was apparently sufficient for fluorescence microscopy, confirmed the entire cuticle to be covered by the tannins. Additionally, the substances seemed to be concentrated at the vulva, the tip of the nose and in the buccal cavity, particularly in those regions lined by a cuticle (Fig.…”

Section: Discussionmentioning

confidence: 97%

Condensed tannins act as anthelmintics by increasing the rigidity of the nematode cuticle

Greiffer

Liebau

Herrmann

et al. 2022

Sci Rep

View full text Add to dashboard Cite

Tannins and tanniferous plant extracts have been discussed as sustainable means for helminth control in the past two decades in response to a dramatic increase of resistances towards standard anthelmintics. While their bioactivities have been broadly investigated in vitro and in vivo, less is known about their mode of action in nematodes, apart from their protein binding properties. In the current study we therefore investigated the impact of a phytochemically well characterized plant extract from Combretum mucronatum, known to contain procyanidins as the active compounds, on the model organism Caenorhabditis elegans. By different microscopic techniques, the cuticle was identified as the main binding site for tannins, whereas underlying tissues did not seem to be affected. In addition to disruptions of the cuticle structure, molting defects occurred at all larval stages. Finally, an increased rigidity of the nematodes’ cuticle due to binding of tannins was confirmed by force spectroscopic measurements. This could be a key finding to explain several anthelmintic activities reported for tannins, especially impairment of molting or exsheathment as well as locomotion.

show abstract

Section: Discussionmentioning

confidence: 97%

Condensed tannins act as anthelmintics by increasing the rigidity of the nematode cuticle

Greiffer

Liebau

Herrmann

et al. 2022

Sci Rep

View full text Add to dashboard Cite

show abstract

“…Polyphenols are a broad class of plant secondary metabolites [1,2]. Thanks to their antioxidant and anti-inflammatory activity, polyphenols are widely used in nutraceuticals and pharmaceutical industry [3], and as diet supplementation in functional feed [4][5][6].…”

Section: Introductionmentioning

confidence: 99%

Comparative Study of Condensed and Hydrolysable Tannins during Early Stages of Zebrafish Development

La Pietra,

Imperatore,

Coccia

et al. 2024

Preprint

View full text Add to dashboard Cite

In this study, we present data on the effects of condensed (CTs) and hydrolysable (HTs) tannins, polyphenols extracted from plants, at different concentrations on zebrafish development to identify the range of concentrations with toxic effects. Zebrafish embryos were exposed to CTs and HTs at two different concentration ranges (5.0, 10.0, 20.0 μgL-1 and 5.0, 10.0, 20.0 mgL-1) for 72 h. The toxicity parameters were observed up to 72 h of treatment. The uptake of CTs and HTs by zebrafish larvae was assessed by HPLC analysis. The qRT-PCR analysis was performed to evaluate gene expression of cd63, zhe1 and klf4, involved in the hatching process of zebrafish. CTs and HTs at 5.0, 10.0, and 20.0 μgL-1 were not toxic. On the contrary, at 5.0, 10.0, and 20.0 mgL-1 HTs induced a delayed in hatching starting from 48 h of treatment, while CTs showed a delayed in hatching mainly at 48h. The analysis of gene expression showed a down-regulation in the group exposed to HTs confirming the hatching data. We believe that this study is important to define the optimal doses of CTs and HTs, to be employed in different application fields such as chemical and animal feed industry, and medical science.

show abstract

“…Polyphenols’ structural variety and wide distribution affects their extraction from the complex plant matrix; for example, insoluble polyphenols and flavonoids are located in the cell wall ( 72 , 73 ). In contrast, soluble phenolic compounds like anthocyanins are found inside the cell vacuoles ( 72 , 74 ). The high temperature of the solvent may promote the distribution of cell wall polysaccharides into the solvent and weaken the integrity of the cell wall, allowing phenolic compounds to pass into the infusion easily ( 75 , 76 ).…”

Section: Introductionmentioning

confidence: 99%

New insights of the application of water or ethanol-water plant extract rich in active compounds in food

Plášková

Mlček

2023

Front. Nutr.

View full text Add to dashboard Cite

Plants are recognized as natural sources of antioxidants (e.g., polyphenols, flavonoids, vitamins, and other active compounds) that can be extracted by green solvents like water, ethanol, or their binary mixtures. Plant extracts are becoming more used as food additives in various food systems due to their antioxidant abilities. Their application in food increases the shelf life of products by preventing undesirable changes in nutritional and sensory properties, such as the formation off-flavors in lipid-rich food. This review summarizes the most recent literature about water or ethanol-water plant extracts used as flavors, colorings, and preservatives to fortify food and beverages. This study is performed with particular attention to describing the benefits of plant extract-fortified products such as meat, vegetable oils, biscuits, pastries, some beverages, yogurt, cheese, and other dairy products. Antioxidant-rich plant extracts can positively affect food safety by partially or fully replacing synthetic antioxidants, which have lately been linked to safety and health issues such as toxicological and carcinogenic consequences. On the other hand, the limitations and challenges of using the extract in food should be considered, like stability, level of purity, compatibility with matrix, price, sensory aspects like distinct taste, and others. In the future, continuous development and a tendency to use these natural extracts as food ingredients are expected, as indicated by the number of published works in this area, particularly in the past decade.

show abstract

Imaging Polyphenolic Compounds in Plant Tissues

Cited by 5 publications

References 75 publications

Condensed tannins act as anthelmintics by increasing the rigidity of the nematode cuticle

Condensed tannins act as anthelmintics by increasing the rigidity of the nematode cuticle

Comparative Study of Condensed and Hydrolysable Tannins during Early Stages of Zebrafish Development

New insights of the application of water or ethanol-water plant extract rich in active compounds in food

Contact Info

Product

Resources

About