Imaging plasma membranes without cellular internalization: multisite membrane anchoring reagents based on glycol chitosan derivatives

Wang, Hong Yin; Hao, Jia; Lü, Xiaolin; Chen, Bin; Zhou, Gaoxin; He, Nongyue; Chen, Zhan; Wu, Fu‐Gen

doi:10.1039/c5tb00930h

Cited by 51 publications

(70 citation statements)

References 70 publications

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“…Glycol chitosan (GC) was purchased from Sigma-Aldrich (St. Louis, MO) and its molecular weight and degree of deacetylation were reported to be 67 kDa and 88%, respectively [50]. Methoxyl PEG2000 succinimidyl ester (NHS-PEG2000-OMe) was bought from Nanocs, Inc. (New York, NY).…”

Section: Methodsmentioning

confidence: 99%

“…In addition, the development of this new amphiphilic construct of GC-PEG-PpIX also promotes the advancement of cell surface engineering, which is a recently emerged field that can modify cell membranes and endow the cells with new properties and functions [50][51][52][53][54][55][56][57][58].…”

Section: Accepted Manuscriptmentioning

confidence: 98%

“…Besides, GC is positively charged at physiological pH and serves as an excellent choice for cell surface modification [50,64]. PEG has been approved by the US Food and Drug Administration (FDA) as a safe hydrophilic…”

Section: Design and Characterization Of Gc-peg-ppix Npsmentioning

confidence: 99%

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Plasma membrane activatable polymeric nanotheranostics with self-enhanced light-triggered photosensitizer cellular influx for photodynamic cancer therapy

Hao

Jiang

Zhu

et al. 2017

Journal of Controlled Release

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Section: Methodsmentioning

confidence: 99%

Section: Accepted Manuscriptmentioning

confidence: 98%

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Plasma membrane activatable polymeric nanotheranostics with self-enhanced light-triggered photosensitizer cellular influx for photodynamic cancer therapy

Hao

Jiang

Zhu

et al. 2017

Journal of Controlled Release

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“…Furthermore, the FITC-labeled glycol chitosan was found to be able to resist cellular internalization and could successfully visualize rafts in live cells. Finally, with their good biocompatibility, 55,56,59,60 glycol chitosan derivatives hold a great promise in biological applications. 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 52 53 54 55 56 57 58 59 60 18 ASSOCIATED CONTENT…”

Section: Resultsmentioning

confidence: 99%

“…55,56 The excellent water solubility of this molecule endows its ease of chemical modification. The conjugation of FITC to glycol Figure S1).…”

mentioning

confidence: 99%

In Situ Visualization of Lipid Raft Domains by Fluorescent Glycol Chitosan Derivatives

et al. 2016

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Lipid rafts are highly ordered small microdomains mainly composed of glycosphingolipids, cholesterol, and protein receptors. Optically distinguishing lipid raft domains in cell membranes would greatly facilitate the investigations on the structure and dynamics of raft-related cellular behaviors, such as signal transduction, membrane transport (endocytosis), adhesion, and motility. However, current strategies about the visualization of lipid raft domains usually suffer from the low biocompatibility of the probes, invasive detection, or ex situ observation. At the same time, naturally derived biomacromolecules have been extensively used in biomedical field and their interaction with cells remains a long-standing topic since it is closely related to various fundamental studies and potential applications. Herein, noninvasive visualization of lipid raft domains in model lipid bilayers (supported lipid bilayers and giant unilamellar vesicles) and live cells was successfully realized in situ using fluorescent biomacromolecules: the fluorescein isothiocyanate (FITC)-labeled glycol chitosan molecules. We found that the lipid raft domains in model or real membranes could be specifically stained by the FITC-labeled glycol chitosan molecules, which could be attributed to the electrostatic attractive interaction and/or hydrophobic interaction between the probes and the lipid raft domains. Since the FITC-labeled glycol chitosan molecules do not need to completely insert into the lipid bilayer and will not disturb the organization of lipids, they can more accurately visualize the raft domains as compared with other fluorescent dyes that need to be premixed with the various lipid molecules prior to the fabrication of model membranes. Furthermore, the FITC-labeled glycol chitosan molecules were found to be able to resist cellular internalization and could successfully visualize rafts in live cells. The present work provides a new way to achieve the imaging of lipid rafts and also sheds new light on the interaction between biomacromolecules and lipid membranes.

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Decoupled pH‐ and Thermo‐Responsive Injectable Chitosan/PNIPAM Hydrogel via Thiol‐Ene Click Chemistry for Potential Applications in Tissue Engineering

Ding

Liu

et al. 2020

Adv Healthcare Materials

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Stimuli‐responsive chitosan (CS) hydrogels exhibit great potential for drug delivery and tissue engineering; however, the structure of these stimuli‐responsive CS hydrogels, such as dual pH‐ and thermo‐responsive hydrogels, is difficult to control or needs additional crosslinking agents. Here, a new dual pH‐ and thermo‐responsive hydrogel system is developed by combining pH‐responsive C6‐OH allyl‐modified CS (OAL‐CS) with thermo‐responsive poly(N‐isopropylacrylamide) (PNIPAM). The thiol groups in PNIPAM and the allyl groups in OAL‐CS can rapidly form crosslinking hydrogel network by “thiol‐ene” click chemistry under UV irradiation. As expected, the swelling ratio of the OAL‐CS/PNIPAM hydrogel can be controlled by changing pH and temperature. Moreover, the hydrogel displays non‐cytotoxic nature toward human bone marrow mesenchymal stem cells, and the histological analyses reveal the subcutaneous tissue with no signs of inflammation after 5 days of injection in vivo. The results indicate that the new OAL‐CS/PNIPAM hydrogel has potential to serve as a smart injectable platform for application in drug delivery and tissue engineering.

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Imaging plasma membranes without cellular internalization: multisite membrane anchoring reagents based on glycol chitosan derivatives

Abstract: Using a multisite membrane anchoring strategy, a new plasma membrane imaging reagent without cellular internalization was designed.

Cited by 51 publications

References 70 publications

Plasma membrane activatable polymeric nanotheranostics with self-enhanced light-triggered photosensitizer cellular influx for photodynamic cancer therapy

Plasma membrane activatable polymeric nanotheranostics with self-enhanced light-triggered photosensitizer cellular influx for photodynamic cancer therapy

In Situ Visualization of Lipid Raft Domains by Fluorescent Glycol Chitosan Derivatives

Decoupled pH‐ and Thermo‐Responsive Injectable Chitosan/PNIPAM Hydrogel via Thiol‐Ene Click Chemistry for Potential Applications in Tissue Engineering

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