Imaging plants dynamics in heterogenic environments

Fiorani, Fabio; Rascher, Uwe; Jahnke, Siegfried; Schurr, Ulrich

doi:10.1016/j.copbio.2011.12.010

Cited by 125 publications

(84 citation statements)

References 53 publications

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“…To achieve increase in yields on available arable land, it must be developed a mechanistic understanding of photosynthetic C-fixation, C-transport and C-allocation to different tissues, and the regulatory system that controls photosynthesis and C-allocation (Evans 2013). Also, a long-standing aim for plant biologists is capturing and interpreting water and sugar flow in plants because of their vital importance for plant growth and life (Hubeau and Steppe 2015;Fiorani et al 2012). In this way, non-invasive imaging methods that analyze the photoassimilates transport and allocation in plants on appropriate spatial and temporal scales can play an important role.…”

Section: Introductionmentioning

confidence: 99%

Imaging of photoassimilates transport in plant tissues by positron emission tomography

Partelová¹,

Kuglerová²,

Konotop³

et al. 2017

Nova Biotechnologica Et Chimica

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Section: Introductionmentioning

confidence: 99%

Imaging of photoassimilates transport in plant tissues by positron emission tomography

Partelová¹,

Kuglerová²,

Konotop³

et al. 2017

Nova Biotechnologica Et Chimica

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“…Accordingly, in most experiments the number of plants harvested per day and treatment usually does not exceed eight (Poorter and Garnier 1996). Imaging solutions using noninvasive approaches to evaluate plant performance are currently implemented (Jansen et al 2009;Fiorani et al 2012) and allow much higher densities of observations. However, most of the imaging methods to date do not yet reach the same precision for large plants as they do for small plants, simply because of overlapping leaves.…”

Section: How Many Replicates Per Treatment?mentioning

confidence: 99%

The art of growing plants for experimental purposes: a practical guide for the plant biologist

Poorter

Fiorani

Stitt

et al. 2012

Functional Plant Biol.

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204

154

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Abstract. Every year thousands of experiments are conducted using plants grown under more-or-less controlled environmental conditions. The aim of many such experiments is to compare the phenotype of different species or genotypes in a specific environment, or to study plant performance under a range of suboptimal conditions. Our paper aims to bring together the minimum knowledge necessary for a plant biologist to set up such experiments and apply the environmental conditions that are appropriate to answer the questions of interest. We first focus on the basic choices that have to be made with regard to the experimental setup (e.g. where are the plants grown; what rooting medium; what pot size). Second, we present practical considerations concerning the number of plants that have to be analysed considering the variability in plant material and the required precision. Third, we discuss eight of the most important environmental factors for plant growth (light quantity, light quality, CO 2 , nutrients, air humidity, water, temperature and salinity); what critical issues should be taken into account to ensure proper growth conditions in controlled environments and which specific aspects need attention if plants are challenged with a certain a-biotic stress factor. Finally, we propose a simple checklist that could be used for tracking and reporting experimental conditions.

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“…Furthermore, these techniques could be very useful for presymptomatic stress detection, depending on the extent in the changes in plants metabolism in response to pathogens. This early detection would allow appropriate measures at the right time, and can be used in the field, on the bench (microscopic or leaf level), as well as on canopy through remote sensing and in high-throughput plant phenotyping platforms [1,2,6,7].…”

Section: Imaging Techniques For Biotic Stress Detectionmentioning

confidence: 99%

“…This limitation has been solved by the use of laser beams. Further improvements in the field include laser-induced fluorescence transients and suninduced fluorescence (passive estimation of Chl-F from solar reflectance spectra) [7,11,98].…”

Section: Future Prospects For Imaging Sensors In Plant Phenotyping Anmentioning

confidence: 99%

Picturing pathogen infection in plants

Barón

Pineda

Pérez-Bueno

2016

Zeitschrift Für Naturforschung C

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Several imaging techniques have provided valuable tools to evaluate the impact of biotic stress on host plants. The use of these techniques enables the study of plant-pathogen interactions by analysing the spatial and temporal heterogeneity of foliar metabolism during pathogenesis. In this work we review the use of imaging techniques based on chlorophyll fluorescence, multicolour fluorescence and thermography for the study of virus, bacteria and fungi-infected plants. These studies have revealed the impact of pathogen challenge on photosynthetic performance, secondary metabolism, as well as leaf transpiration as a promising tool for field and greenhouse management of diseases. Images of standard chlorophyll fluorescence (Chl-F) parameters obtained during Chl-F induction kinetics related to photochemical processes and those involved in energy dissipation, could be good stress indicators to monitor pathogenesis. Changes on UV-induced blue (F440) and green fluorescence (F520) measured by multicolour fluorescence imaging in pathogen-challenged plants seem to be related with the up-regulation of the plant secondary metabolism and with an increase in phenolic compounds involved in plant defence, such as scopoletin, chlorogenic or ferulic acids. Thermal imaging visualizes the leaf transpiration map during pathogenesis and emphasizes the key role of stomata on innate plant immunity. Using several imaging techniques in parallel could allow obtaining disease signatures for a specific pathogen. These techniques have also turned out to be very useful for presymptomatic pathogen detection, and powerful non-destructive tools for precision agriculture. Their applicability at lab-scale, in the field by remote sensing, and in high-throughput plant phenotyping, makes them particularly useful. Thermal sensors are widely used in crop fields to detect early changes in leaf transpiration induced by both air-borne and soil-borne pathogens. The limitations of measuring photosynthesis by Chl-F at the canopy level are being solved, while the use of multispectral fluorescence imaging is very challenging due to the type of light excitation that is used.Keywords: biotic stress; chlorophyll fluorescence imaging; multicolor fluorescence imaging; plant pathogens; thermography. Dedication: This work is dedicated to the memory of Professor Peter Böger. He will always bring to my heart (M. B.) the best memories of my postdoc at Lake Constance.

show abstract

Imaging plants dynamics in heterogenic environments

Cited by 125 publications

References 53 publications

Imaging of photoassimilates transport in plant tissues by positron emission tomography

Imaging of photoassimilates transport in plant tissues by positron emission tomography

The art of growing plants for experimental purposes: a practical guide for the plant biologist

Picturing pathogen infection in plants

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