Imaging performance of a miniature integrated microendoscope

Rogers, Jeremy D.; Landau, Sara; Tkaczyk, Tomasz S.; Descour, Michael R.; Rahman, Mohammed; Richards‐Kortum, Rebecca; Kärkäinen, Ari H. O.; Christenson, Todd

doi:10.1117/1.2978060

Cited by 12 publications

(14 citation statements)

References 26 publications

(20 reference statements)

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“…We created a miniaturized optical pathway, a data acquisition system, three circuit boards, and a housing with fine focusing capability, while keeping the microscope to 1.9 g mass. Prior approaches to fluorescence imaging in miniaturized format have generally required accessory, tabletop optical instrumentation 6,11,12,31 . An earlier integrated system for use in mobile rats exhibited a Nyquist resolution of ∼20 μm, insufficient for observing individual cells 32 .…”

Section: Discussionmentioning

confidence: 99%

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Miniaturized integration of a fluorescence microscope

et al. 2011

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The light microscope is traditionally an instrument of substantial size and expense. Its miniaturized integration would enable many new applications based on mass-producible, tiny microscopes. Key prospective usages include brain imaging in behaving animals towards relating cellular dynamics to animal behavior. Here we introduce a miniature (1.9 g) integrated fluorescence microscope made from mass-producible parts, including semiconductor light source and sensor. This device enables high-speed cellular-level imaging across ∼0.5 mm2 areas in active mice. This capability allowed concurrent tracking of Ca2+ spiking in >200 Purkinje neurons across nine cerebellar microzones. During mouse locomotion, individual microzones exhibited large-scale, synchronized Ca2+ spiking. This is a mesoscopic neural dynamic missed by prior techniques for studying the brain at other length scales. Overall, the integrated microscope is a potentially transformative technology that permits distribution to many animals and enables diverse usages, such as portable diagnostics or microscope arrays for large-scale screens.

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Section: Discussionmentioning

confidence: 99%

“…Past efforts to address these have yielded miniaturized devices that needed accessory, conventional optical instrumentation 11,12 or that did not permit fluorescence imaging and made specialized assumptions about the specimen 13,14 .…”

Section: Introductionmentioning

confidence: 99%

Miniaturized integration of a fluorescence microscope

et al. 2011

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“…• Benign esophagus modifications preserve the honeycomb pattern of the columnar mucosa but polypoid-like projections similar to the gastric mucosa appear. • The appearance of round bright or dark (with or without mucin content, respectively) features approximately [10][11][12][13][14][15] µm in diameter are the optical signature of goblet cells and thus, the fingerprint of Barrett's esophagus. Typically, a columnar mucosa with scattered goblet cells is observed but the surface retains a degree of normality and smoothness.…”

Section: Development Of Rules For Image Interpretationmentioning

confidence: 99%

“…The combination of targeted peptide probes for complementary functional confocal data has also been explored 8 . Non-confocal endomicroscopy prototype systems include the use of contrast agents such as acriflavine hydrochloride 9 , as well as quantum dots and gold nanoparticles 10 . Wide-field narrow-band imaging (NBI) has been investigated without the use of contrast agents 11 .…”

Section: Introductionmentioning

confidence: 99%

Translating ultraviolet autofluorescence microscopy toward clinical endomicroscopy

et al. 2011

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The potential of autofluorescence microscopy under ultraviolet excitation is investigated as a method to visualize superficial epithelial microstructures and their modification with progression of disease. This method does not require the use of contrast agents, sectioning methods, or tissue preparation. Imaging of esophagus tissue is the focus of this study and deals with three main issues: a) What is the origin of the signal; b) How the gradual microstructure modification associated with various stages of esophageal disease is visualized; c) What are the designing parameters for in vivo implementation.

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“…These applications include endoscope-compatible in vivo microscopes such as confocal reflectance and fluorescence [1–3], structured illumination [4], and multi- and two-photon [5–7] systems. There is also growing interest in applying miniature optics for portable point-of-care diagnostics [8] and needle sized optical biopsy systems [9].…”

Section: Introductionmentioning

confidence: 99%

Low cost, high performance, self-aligning miniature optical systems

Kester

Christenson²,

Kortum

et al. 2009

Appl. Opt.

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The most expensive aspects in producing high quality miniature optical systems are the component costs and long assembly process. A new approach for fabricating these systems that reduces both aspects through the implementation of self-aligning LIGA (German acronym for lithographie, galvanoformung, abformung, or x-ray lithography, electroplating, and molding) optomechanics with high volume plastic injection molded and off-the-shelf glass optics is presented. This zero alignment strategy has been incorporated into a miniature high numerical aperture (NA = 1.0W) microscope objective for a fiber confocal reflectance microscope. Tight alignment tolerances of less than 10 μm are maintained for all components that reside inside of a small 9 gauge diameter hypodermic tubing.A prototype system has been tested using the slanted edge modulation transfer function technique and demonstrated to have a Strehl ratio of 0.71. This universal technology is now being developed for smaller, needle-sized imaging systems and other portable point-of-care diagnostic instruments.

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Imaging performance of a miniature integrated microendoscope

Cited by 12 publications

References 26 publications

Miniaturized integration of a fluorescence microscope

Miniaturized integration of a fluorescence microscope

Translating ultraviolet autofluorescence microscopy toward clinical endomicroscopy

Low cost, high performance, self-aligning miniature optical systems

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