Imaging of tumor colonization by <i>Escherichia coli</i> using <sup>18</sup>F-FDS PET

Kang, Sae-Ryung; Jo, Eui Jeong; Nguyen, Vu H.; Zhang, Ying; Yoon, Hee Seung; Pyo, Ayoung; Kim, Dong-Yeon; Hong, Yeongjin; Bom, Hee‐Seung; Min, Jung‐Joon

doi:10.7150/thno.42121

Cited by 47 publications

(37 citation statements)

References 27 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Indeed, several groups showed a high specificity of [ 18 F]FDS binding to E. coli or K. pneumoniae [28] in animals. Several other studies have demonstrated similar results, but always in animal models [29,30]. In addition, other sugars such as 18 F-fluoromaltohexaose (FMH) [31], 6 -18 F-fluoromaltotriose and 18 F-fluoroacetamido-d-glucopyranose (FAG), [32] were revealed to be sensitive and specific radiopharmaceuticals for the detection of E. coli [33].…”

Section: Imaging Bacteria In Animal Modelsmentioning

confidence: 61%

Imaging Bacteria with Radiolabelled Probes: Is It Feasible?

Artiko

Conserva

Ferro-Flores

et al. 2020

JCM

View full text Add to dashboard Cite

Bacterial infections are the main cause of patient morbidity and mortality worldwide. Diagnosis can be difficult and delayed as well as the identification of the etiological pathogen, necessary for a tailored antibiotic therapy. Several non-invasive diagnostic procedures are available, all with pros and cons. Molecular nuclear medicine has highly contributed in this field by proposing several different radiopharmaceuticals (antimicrobial peptides, leukocytes, cytokines, antibiotics, sugars, etc.) but none proved to be highly specific for bacteria, although many agents in development look promising. Indeed, factors including the number and strain of bacteria, the infection site, and the host condition, may affect the specificity of the tested radiopharmaceuticals. At the Third European Congress on Infection/Inflammation Imaging, a round table discussion was dedicated to debate the pros and cons of different radiopharmaceuticals for imaging bacteria with the final goal to find a consensus on the most relevant research steps that should be fulfilled when testing a new probe, based on experience and cumulative published evidence.

show abstract

Section: Imaging Bacteria In Animal Modelsmentioning

confidence: 61%

Imaging Bacteria with Radiolabelled Probes: Is It Feasible?

Artiko

Conserva

Ferro-Flores

et al. 2020

JCM

View full text Add to dashboard Cite

show abstract

“…Furthermore, E. coli MG1655 has also been reported to be selective colonizers of tumor microenvironments and have been shown to exert weak (wild‐type) to strong (engineered) tumor and metastasis inhibition effects. [ 100,101 ] Nevertheless, whether these activities are generated from sirtuin activation or other parallel mechanisms requires specific investigations. Escherichia sp .…”

Section: Resultsmentioning

confidence: 99%

In silico insights into potential gut microbial modulation of NAD+ metabolism and longevity

Salekeen

Siam

Sharif

et al. 2021

J Biochem & Molecular Tox

View full text Add to dashboard Cite

Recent evidence has prompted the notion of gut‐microbial signatures as an indirect marker of aging and aging‐associated decline in humans. However, the underlying host‐symbiont molecular interactions contributing to these signatures remain poorly understood. In this study, we address this gap using cheminformatic analyses to elucidate potential gut microbial metabolites that may perturb the longevity‐associated NAD+ metabolic network. In silico ADMET, KEGG interaction analysis, molecular docking, molecular dynamics simulation, and molecular mechanics calculation predict a large number of safe and bioavailable microbial metabolites to be direct and/or indirect activators of NAD+‐dependent sirtuin proteins. Our simulation results suggest dihydropteroate, phenylpyruvic acid, indole‐3‐propionic acid, phenyllactic acid, all‐trans‐retinoic acid, and multiple deoxy‐, methyl‐, and cyclic nucleotides from intestinal microbiota as the best‐performing regulators of NAD+ metabolism. Retracing these molecules to their source microorganisms also suggest commensal Escherichia, Bacteroides, Bifidobacteria, and Lactobacilli to be associated with the highest number of pro‐longevity metabolites. These findings from our early‐stage study, therefore, provide an informatics‐based context for previous evidence in the area and grant novel insights for future clinical investigation intersecting anti‐aging drug discovery, probiotics, and gut microbial signatures.

show abstract

“…[ 18 F]-FDS, a synthetic analog of [ 18 F]-FDG, has been shown to accumulate in Gram-negative Enterobacteriaceae selectively but not in mammalian or cancer cells. In PET imaging, the uptake of radioactivity in the tumor had a positive relationship with the number of viable bacteria, allowing a semiquantitative measure of bacterial density in the tumor [ 51 ] ( Figure 2 ). The successful visualization and quantification of therapeutic E. coli by [ 18 F]-FDS will make it possible to predict the therapeutic response, which could facilitate the clinical translation of bacteria-mediated tumor therapy.…”

Section: Ecn-mediated Tumor Imagingmentioning

confidence: 99%

Escherichiacoli Nissle 1917 as a Novel Microrobot for Tumor-Targeted Imaging and Therapy

et al. 2021

View full text Add to dashboard Cite

Highly efficient drug delivery systems with excellent tumor selectivity and minimal toxicity to normal tissues remain challenging for tumor treatment. Although great effort has been made to prolong the blood circulation and improve the delivery efficiency to tumor sites, nanomedicines are rarely approved for clinical application. Bacteria have the inherent properties of homing to solid tumors, presenting themselves as promising drug delivery systems. Escherichia coli Nissle 1917 (EcN) is a commonly used probiotic in clinical practice. Its facultative anaerobic property drives it to selectively colonize in the hypoxic area of the tumor for survival and reproduction. EcN can be engineered as a bacteria-based microrobot for molecular imaging, drug delivery, and gene delivery. This review summarizes the progress in EcN-mediated tumor imaging and therapy and discusses the prospects and challenges for its clinical application. EcN provides a new idea as a delivery vehicle and will be a powerful weapon against cancer.

show abstract

Imaging of tumor colonization by Escherichia coli using ¹⁸F-FDS PET

Cited by 47 publications

References 27 publications

Imaging Bacteria with Radiolabelled Probes: Is It Feasible?

Imaging Bacteria with Radiolabelled Probes: Is It Feasible?

In silico insights into potential gut microbial modulation of NAD+ metabolism and longevity

Escherichiacoli Nissle 1917 as a Novel Microrobot for Tumor-Targeted Imaging and Therapy

Contact Info

Product

Resources

About

Imaging of tumor colonization by Escherichia coli using 18F-FDS PET

Cited by 47 publications

References 27 publications

Imaging Bacteria with Radiolabelled Probes: Is It Feasible?

Imaging Bacteria with Radiolabelled Probes: Is It Feasible?

In silico insights into potential gut microbial modulation of NAD+ metabolism and longevity

Escherichiacoli Nissle 1917 as a Novel Microrobot for Tumor-Targeted Imaging and Therapy

Contact Info

Product

Resources

About

Imaging of tumor colonization by Escherichia coli using ¹⁸F-FDS PET