Imaging of transparent spheres through a planar interface using a high-numerical-aperture optical microscope

Ovryn, Ben; Izen, Steven H.

doi:10.1364/josaa.17.001202

Cited by 57 publications

(46 citation statements)

References 23 publications

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“…2͑a͔͒. 29,30 For that a sequence of images is recorded ͑repetition rate of 30 Hz͒ and analyzed based on the Levenberg-Marquardt algorithm ͓Fig. 2͑b͔͒.…”

Section: B Data Analysis and Calibrationmentioning

confidence: 99%

Colloids dragged through a polymer solution: Experiment, theory, and simulation

Gutsche

Kremer

Krüger

et al. 2008

The Journal of Chemical Physics

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Document VersionPublisher's PDF, also known as Version of Record (includes final page, issue and volume numbers)Please check the document version of this publication:• A submitted manuscript is the author's version of the article upon submission and before peer-review. There can be important differences between the submitted version and the official published version of record. People interested in the research are advised to contact the author for the final version of the publication, or visit the DOI to the publisher's website.• The final author version and the galley proof are versions of the publication after peer review.• The final published version features the final layout of the paper including the volume, issue and page numbers. Link to publication General rightsCopyright and moral rights for the publications made accessible in the public portal are retained by the authors and/or other copyright owners and it is a condition of accessing publications that users recognise and abide by the legal requirements associated with these rights.• Users may download and print one copy of any publication from the public portal for the purpose of private study or research.• You may not further distribute the material or use it for any profit-making activity or commercial gain • You may freely distribute the URL identifying the publication in the public portal ? Take down policyIf you believe that this document breaches copyright please contact us providing details, and we will remove access to the work immediately and investigate your claim. We present microrheological measurements of the drag force on colloids pulled through a solution of -DNA ͑used here as a monodisperse model polymer͒ with an optical tweezer. The experiments show a drag force that is larger than expected from the Stokes formula and the independently measured viscosity of the DNA solution. We attribute this to the accumulation of DNA in front of the colloid and the reduced DNA density behind the colloid. This hypothesis is corroborated by a simple drift-diffusion model for the DNA molecules, which reproduces the experimental data surprisingly well, as well as by corresponding Brownian dynamics simulations.

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“…2͑a͔͒. 29,30 For that a sequence of images is recorded ͑repetition rate of 30 Hz͒ and analyzed based on the Levenberg-Marquardt algorithm ͓Fig. 2͑b͔͒.…”

Section: B Data Analysis and Calibrationmentioning

confidence: 99%

Colloids dragged through a polymer solution: Experiment, theory, and simulation

Gutsche

Kremer

Krüger

et al. 2008

The Journal of Chemical Physics

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“…Ovryn and Izen [13] and Lee et al [14] measured positions and optical properties of spherical colloidal particles by fitting a Lorenz-Mie scattering solution to holograms, taking the particle position, radius, and refractive index as fitting parameters. Fitting holograms using scattering solutions takes advantage of known information about the particle, such as its shape, and avoids artifacts that lead to systematic errors [15].…”

Section: Introductionmentioning

confidence: 99%

Random-subset fitting of digital holograms for fast three-dimensional particle tracking [Invited]

et al. 2014

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“…While we only demonstrate our method on a single imaging system-3D line-scanning confocal images of nearly monodisperse spheres in a dyed fluid-the ideas behind our technique are generic and should apply to a wide range of imaging modalities. As a first step, extending PERI to describe image formation in brightfield microscopy by correctly describing aberrations and light scattering from the sample [49] would provide nanometer-scale precision for a simple and widespread imaging setup. Creating an accurate generative model of TEM or STM, by describing the focusing and scattering of the electron beam [50] or understanding the STM probe tip's response [51], could provide unprecedented detail of atomic positions.…”

Section: Discussionmentioning

confidence: 99%

Light Microscopy at Maximal Precision

et al. 2017

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Microscopy is the workhorse of the physical and life sciences, producing crisp images of everything from atoms to cells well beyond the capabilities of the human eye. However, the analysis of these images is frequently little more accurate than manual marking. Here, we revolutionize the analysis of microscopy images, extracting all the useful information theoretically contained in a complex microscope image. Using a generic, methodological approach, we extract the information by fitting experimental images with a detailed optical model of the microscope, a method we call parameter extraction from reconstructing images (PERI). As a proof of principle, we demonstrate this approach with a confocal image of colloidal spheres, improving measurements of particle positions and radii by 10-100 times over current methods and attaining the maximum possible accuracy. With this unprecedented accuracy, we measure nanometer-scale colloidal interactions in dense suspensions solely with light microscopy, a previously impossible feat. Our approach is generic and applicable to imaging methods from brightfield to electron microscopy, where we expect accuracies of 1 nm and 0.1 pm, respectively.

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Imaging of transparent spheres through a planar interface using a high-numerical-aperture optical microscope

Cited by 57 publications

References 23 publications

Colloids dragged through a polymer solution: Experiment, theory, and simulation

Colloids dragged through a polymer solution: Experiment, theory, and simulation

Random-subset fitting of digital holograms for fast three-dimensional particle tracking [Invited]

Light Microscopy at Maximal Precision

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