Imaging of the Ryanodine Receptor Distribution in Rat Cardiac Myocytes with Optical Single Channel Resolution

Abstract

1Hz, room temperature) into nucleoplasmic and cytoplasmic [Ca 2þ ]. There was a significant difference in diastolic (121524nM vs 149535nM; 99517nM vs 121526nM) and systolic (4205148nM vs 3645102nM; 7875172nM vs 4915157nM) [Ca 2þ ] between cytoplasmic and nucleoplasmic compartments in mouse and rat cells, respectively (both n¼15; P<0.01). The results reveal that, in cardiac myocytes, the Ca 2þ -dependent fluorescent properties of Fluo-4 differ between cytoplasm and nucleoplasm and that significant differences between cytoplasmic and nucleoplasmic [Ca 2þ ] exist during diastole as well as systole.

The Interplay of Rogue and Clustered Ryanodine Receptors Regulates Ca2+ Waves in Cardiac Myocytes

“…Figure 1A shows the geometrical model of a cardiac myocyte, the x- and y-directions of which refer to the longitudinal axis and z-line, respectively. Baddeley et al have experimentally observed the RyR distribution on the JSR membrane (Baddeley et al, 2010 ). Most RyR channels form regular arrays, defined as “clustered RyRs.” Others are rogue RyRs uncoupled from the clustered RyRs.…”