Imaging of Plant Hormones with Nanoparticle-Assisted Laser Desorption/Ionization Mass Spectrometry

Taira, Shu; Shiono, Katsuhiro

doi:10.1007/978-1-0716-2030-4_9

Cited by 2 publications

(2 citation statements)

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“…To visualize ACC and di-ACC in planta we made use of Nano Particle-Assisted Laser Desorption/Ionization mass spectrometry imaging (Nano-PALDI MSI), which has recently been used to identify the spatial distribution of hormones and metabolites in plant tissue sections (Shiono et al, 2017 ; Ortigosa et al, 2022 ; Taira and Shiono, 2022 ). Ten day-old Arabidopsis seedlings were treated with 2 μM ACC, di-ACC or both overnight, before hypocotyl sections were analyzed using Nano-PALDI MS imaging ( Figure 2C ).…”

Section: Resultsmentioning

confidence: 99%

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A 1-aminocyclopropane-1-carboxylic-acid (ACC) dipeptide elicits ethylene responses through ACC-oxidase mediated substrate promiscuity

Vaughan-Hirsch

Martinez

et al. 2022

Front. Plant Sci.

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Plants produce the volatile hormone ethylene to regulate many developmental processes and to deal with (a)biotic stressors. In seed plants, ethylene is synthesized from 1-aminocyclopropane-1-carboxylic acid (ACC) by the dedicated enzyme ACC oxidase (ACO). Ethylene biosynthesis is tightly regulated at the level of ACC through ACC synthesis, conjugation and transport. ACC is a non-proteinogenic amino acid, which also has signaling roles independent from ethylene. In this work, we investigated the biological function of an uncharacterized ACC dipeptide. The custom-synthesized di-ACC molecule can be taken up by Arabidopsis in a similar way as ACC, in part via Lysine Histidine Transporters (e.g., LHT1). Using Nano-Particle Assisted Laser Desoprtion/Ionization (Nano-PALDI) mass-spectrometry imaging, we revealed that externally fed di-ACC predominantly localizes to the vasculature tissue, despite it not being detectable in control hypocotyl segments. Once taken up, the ACC dimer can evoke a triple response phenotype in dark-grown seedlings, reminiscent of ethylene responses induced by ACC itself, albeit less efficiently compared to ACC. Di-ACC does not act via ACC-signaling, but operates via the known ethylene signaling pathway. In vitro ACO activity and molecular docking showed that di-ACC can be used as an alternative substrate by ACO to form ethylene. The promiscuous nature of ACO for the ACC dimer also explains the higher ethylene production rates observed in planta, although this reaction occurred less efficiently compared to ACC. Overall, the ACC dipeptide seems to be transported and converted into ethylene in a similar way as ACC, and is able to augment ethylene production levels and induce subsequent ethylene responses in Arabidopsis.

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Section: Resultsmentioning

confidence: 99%

“…Nano-PALDI MSI was carried out as previously described (Shiono et al, 2017 ; Ortigosa et al, 2022 ; Taira and Shiono, 2022 ), but with a few modifications for small tissues. Briefly, 10 day-old seedlings of Arabidopsis ecotype Col-0 were incubated in 2 μM ACC or di-ACC overnight.…”

Section: Methodsmentioning

confidence: 99%