Journal of Cell Biology volume 217, issue 4, P1537-1552 2018 DOI: 10.1083/jcb.201709153 View full text
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Sascha Conic, Dominique Desplancq, Alexia Ferrand, Veronique Fischer, Vincent Heyer, Bernardo Reina San Martin, Julien Pontabry, Mustapha Oulad-Abdelghani, Kishore Babu N., Graham D. Wright, Nacho Molina, Etienne Weiss, László Tora

Abstract: Conic et al. introduce a versatile antibody-based imaging approach to track endogenous nuclear factors in living cells. It allows efficient intracellular delivery of any fluorescent dye–conjugated antibody, or Fab fragment, into a variety of cell types. The dynamics of nuclear targets or posttranslational modifications can be monitored with high precision using confocal and super-resolution microscopy.