Imaging of Myocardial Ischemia–Reperfusion Injury Using Sodium [¹⁸F]Fluoride Positron Emission Tomography/Computed Tomography in Rats and Humans

Abstract

Positron emission tomography (PET)/computed tomography (CT) using sodium [18F]fluoride (Na[18F]F) has been proven to be a promising hot-spot imaging modality for myocardial infarction (MI). We investigated Na[18F]F uptake in ischemia–reperfusion injury (IRI) of rats and humans. Sodium [18F]fluoride PET/CT was performed in Sprague-Dawley rats that had IRI surgery, and it readily demonstrated prominent Na[18F]F uptake in the infarct area post-IRI. Sodium [18F]fluoride uptake was matched with negative 2,3,5-triphenyl-2H-tetrazolium chloride staining results, accompanied by myocardial apoptosis and associated with positive calcium staining results. Furthermore, area at risk was negative for Na[18F]F uptake. Cyclosporine A (CysA) treatment reduced standardized uptake value of 18F over the infarct area, and a significant decrease in infarct size was also observed by the CysA treatment. In humans, Na[18F]F PET/CT readily demonstrated increased Na[18F]F uptake in the 2 patients with MI post-percutaneous coronary intervention. In conclusion, this study sheds light on the potential utility of Na[18F]F PET/CT as a hot-spot imaging modality for myocardial IRI.

The Effect of Chinese Medicine on Lipid and Glucose Metabolism in Acute Myocardial Infarction Through PPARγ Pathway

“…Results showed that glucose was accumulated abnormally in peri-infarct area in HF model group, whereas that in the sham group was intact. The abnormal increase of glucose in peri-infarct area in model group may be due to compensatory increasing glucose uptake from blood into the heart and local inflammatory response in the myocardium with low energy metabolism, which is consistent with the previous study ( Tarkia et al, 2015 ; Choi et al, 2017 ), The accumulating glucose was significantly metabolized after treatment with DQP and RSG (Figure 3B ). SUV in the model group increased by 395.15% compared with those in the sham group ( P < 0.001).…”

Longitudinal evaluation of myocardial glucose metabolism and contractile function in obese type 2 diabetic db/db mice using small-animal dynamic 18F-FDG PET and echocardiography

“…After the PET scan, a CT study was acquired and the list-mode PET data were reconstructed using a filtered back projection algorithm with CT-based attenuation correction. Images were analyzed in a blinded manner using PMOD 3.6 software (PMOD Technologies Ltd., Zürich, Switzerland), which provides the quantification of dynamic PET of the heart with a broad range of tracers in small animals including rodents [ 46 , 58 , 59 ]. In brief, volumes of interest (VOI) were drawn semi-automatically by use of a center-line within the myocardium in the projection of three dimensions (short-axis, vertical long, horizontal-long).…”

Inhibition of miR‐148b ameliorates myocardial ischemia/reperfusion injury via regulation of Wnt/β‐catenin signaling pathway

“…Sodium [ 18 F] fluoride uptake was quantitated with the standardized uptake value (SUV) over the infarct area. SUV was calculated as described previously (Choi et al, 2017).…”