Imaging of liver function with dedicated animal dynamic pinhole scintigraphy in rats

Bennink, Roelof J.; Veteläinen, Reeta; Bruin, Kora de; Vliet, Arlène K. van; Gulik, Thomas M. van

doi:10.1097/01.mnm.0000183794.59097.0b

Cited by 11 publications

(15 citation statements)

References 21 publications

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“…HBS scans were performed after 24 h reperfusion with an overnight fast (food ingestion stimulates hepatic blood and bile flow) as described before [13]. Briefly, a bolus of 40 MBq 99m Tc-labeled (2,4,6-trimethyl-3-bromo) iminodiacetic acid ( 99m Tc-mebrofenin, Bridatec, GE-Amersham Health, London, UK) in 0.3 mL saline was administered through the tail vein.…”

Section: Hepatobiliary Scintigraphymentioning

confidence: 99%

Quantitative Assessment of Liver Function after Ischemia-Reperfusion Injury and Partial Hepatectomy in Rats

Graaf

Heger

Spruijt

et al. 2012

Journal of Surgical Research

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Section: Hepatobiliary Scintigraphymentioning

confidence: 99%

Quantitative Assessment of Liver Function after Ischemia-Reperfusion Injury and Partial Hepatectomy in Rats

Graaf

Heger

Spruijt

et al. 2012

Journal of Surgical Research

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“…The use of the hepatic 99m Tc-mebrofenin uptake rate measured by dedicated pinhole HBS has recently been validated in different rat models of liver regeneration (77). 99m Tc-mebrofenin HBS proved to be an accurate, noninvasive tool for the measurement of liver function in the rat and enabled serial measurements within the same animal (77).…”

Section: Hbs In Experimental Surgical Researchmentioning

confidence: 99%

“…Alternative methods for determining liver function include hepatic extraction fraction, the time at which maximal hepatic radioactivity occurs (T peak ), and the time required for peak activity to decrease by 50% (T 1/2 peak ) (77)(78)(79). The hepatic extraction fraction is calculated from the time-activity curves of the heart and liver by a deconvulsion analysis using a (modified) Fourier transform method (80).…”

Section: The Kinetics and Quantitative Measurement Of Liver Functionmentioning

confidence: 99%

Nuclear Imaging Techniques for the Assessment of Hepatic Function in Liver Surgery and Transplantation

Graaf¹,

Bennink²,

Veteläinen³

et al. 2010

J Nucl Med

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139

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This review describes the application of 2 nuclear imaging techniques for assessment of hepatic function in the setting of liver surgery and transplantation. The biochemical and technical background, as well as the clinical applications, of 99m Tc-labeled diethylenetriaminepentaacetic acid galactosyl human serum albumin (GSA) scintigraphy and hepatobiliary scintigraphy (HBS) with 99m Tc-labeled iminodiacetic acid derivates is discussed. 99m Tc-mebrofenin is considered the most suitable iminodiacetic acid agent for 99m Tc-HBS. 99m Tc-GSA scintigraphy and 99m Tcmebrofenin HBS are based on 2 different principles. 99m Tc-GSA scintigraphy is a receptor-mediated technique whereas HBS represents hepatic uptake and excretion function. Both techniques are noninvasive and provide visual and quantitative information on both total and regional liver function. They can be used for preoperative assessment of future remnant liver function, follow-up after preoperative portal vein embolization, and evaluation of postoperative liver regeneration. In liver transplantation, these methods are used to assess graft function and biliary complications.

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“…The hepatic 99m Tc-mebrofenin uptake rate (%/min) was calculated from 3 different time-activity curves obtained from regions of interest around the liver, mediastinum (the blood pool), and total field of view using radioactivity values acquired between 30 and 120 s after injection (22,23). A liver region of interest, that excluded large bile ducts and superimposed bowel loops, was established to create a hepatic time-activity curve for the calculation of the excretory liver function.…”

Section: Icg Clearance Testmentioning

confidence: 99%

Quantitative Assessment of Hepatic Function During Liver Regeneration in a Standardized Rat Model

et al. 2011

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Reliable assessment of hepatic function during liver regeneration is crucial after liver surgery or liver transplantation. 99m Tcgalactosyl human serum albumin ( 99m Tc-GSA) scintigraphy, 99m Tc-mebrofenin hepatobiliary scintigraphy (HBS), the indocyanine green (ICG) clearance test, and the galactose elimination capacity (GEC) are common quantitative liver function tests. However, to our knowledge, comparative analysis between these tests has never been performed during liver regeneration. The aim of this study was therefore to compare 99m Tc-GSA scintigraphy, 99m Tc-mebrofenin HBS, the ICG clearance test, and GEC in the assessment of hepatic function during liver regeneration in rats. Methods: Rats were subjected to 70% partial hepatectomy (PHx). Liver function and functional volume were determined at predefined times after PHx and expressed as a percentage of baseline (pre-PHx) values. Results: During liver regeneration, functional liver volume measured by 99m Tc-GSA SPECT correlated strongly with conventional liver volume based on wet weight. One day after 70% PHx, conventional liver volume overestimated liver function as measured by 99m Tc-mebrofenin uptake and excretion rates, 99m Tc-GSA uptake rate, and ICG clearance. On days 5 and 7, 99m Tc-mebrofenin uptake and ICG clearance had recovered to levels similar to conventional liver volume, whereas 99m Tc-GSA uptake reflected a reduced liver function in relation to conventional liver volume and 99m Tc-mebrofenin uptake. The GEC measurements consistently overestimated liver function during regeneration. Conclusion: Volumetric assessment of the liver should be complemented by liver function-specific assays. Functional regeneration is impaired, compared with volumetric regeneration, in the early phase of liver regeneration. In later stages of liver regeneration, 99m Tc-GSA uptake underestimates hepatic regeneration in comparison to liver volume and 99m Tc-mebrofenin uptake. GEC is of less value because it is only minimally affected by 70% PHx and is influenced by factors not related to liver function. 99m Tc-mebrofenin HBS has the advantage of providing visual and quantitative information regarding both uptake and excretory liver function.

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Imaging of liver function with dedicated animal dynamic pinhole scintigraphy in rats

Abstract: HBS offers a unique combination of functional liver uptake and excretion assessment with the ability to determine the liver function reserve before and after an intervention in rats.

Cited by 11 publications

References 21 publications

Quantitative Assessment of Liver Function after Ischemia-Reperfusion Injury and Partial Hepatectomy in Rats

Quantitative Assessment of Liver Function after Ischemia-Reperfusion Injury and Partial Hepatectomy in Rats

Nuclear Imaging Techniques for the Assessment of Hepatic Function in Liver Surgery and Transplantation

Quantitative Assessment of Hepatic Function During Liver Regeneration in a Standardized Rat Model

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