Imaging of iris vasculature: current limitations and future perspective

Iovino, Claudio; Peiretti, Enrico; Braghiroli, Mirco; Tatti, Filippo; Aloney, Abhilasha; Lanza, Michele; Chhablani, Jay

doi:10.1038/s41433-021-01809-2

Cited by 13 publications

(6 citation statements)

References 73 publications

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“…Au-CS@FNDs CLs were found to possess more water content than the pristine ones; this could be attributed to chitosan's strong water absorption moisturizing ability 64 . Gold nanoparticles have been widely used in photodynamic therapy due to their excellent photothermal conversation effect 65 . The photothermal conversation effect of FNDs, Au-CS, and Au-CS@FNDs was evaluated (Figure S6.)…”

Section: Antibacterial Activities Of Fnds Au-cs and Au-cs@fnds Nanopa...mentioning

confidence: 99%

“…The growth of new blood vessels from the capillaries and venules of the peripheral vascular plexus can block lights entering the eyes and affect vision 65,65 . The corneal neovascular (CNW) can be secondary to excessive reactive oxygen species with eye drops, inflammation, chemistry burns, trauma, etc.…”

Section: Inhibition Of Hvecs By Photothermal Effect Of Au-cs@fnds Clsmentioning

confidence: 99%

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“Shin in the eye”: Au-CS@FNDs as contact lens additives for blocking UV irradiation, bacterial keratitis, and corneal neovascularization therapies

Nie¹,

Hu²,

Zhao

et al. 2023

Preprint

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Ophthalmic disease treatment remains a significant problem globally, resulting in poor vision and blindness. UV irradiation and bacterial infection may cause severe damage to the corneal, leading to vision loss within a few days. Corne-al neovascular abnormally growth that bock lights reach to eyes, causes low vision. Thus it requires urgent and efficient clinical treatment. Contact lenses play an essential role in treating ophthalmic issues. In this work, we synthesized the chitosan-stabilized Au nanoparticles with a simple method. The Au nanoparticles were further physically adsorbed with negatively charged fluorescent nanodiamonds, yielding Au-CS@FNDs. These Au-CS@FNDs particles were proven with excellent UV adsorption, antibacterial properties, and photothermal conversation ability. Furthermore, we embedded Au-CS@FNDs particles into contact lenses to prevent corneal damage from UV light and bacterial infection. Moreover, the Au-CS@FNDs embedded contact lenses were used to inhibit the neovascularization in the Human Vascular Endothelial Cells via the photodynamic effect of Au nanoparticles. To the best of our knowledge, it is the first time that gold and diamond nanoparticles were used as additives in contact lenses, aiming at clinically corneal neovascular. Our results suggest that the controllable photothermal effect of Au-CS@FNDs embedded contact lenses may provide a unique way to intervene the neovascular-induced vision loss.

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Section: Antibacterial Activities Of Fnds Au-cs and Au-cs@fnds Nanopa...mentioning

confidence: 99%

Section: Inhibition Of Hvecs By Photothermal Effect Of Au-cs@fnds Clsmentioning

confidence: 99%

“Shin in the eye”: Au-CS@FNDs as contact lens additives for blocking UV irradiation, bacterial keratitis, and corneal neovascularization therapies

Nie¹,

Hu²,

Zhao

et al. 2023

Preprint

View full text Add to dashboard Cite

show abstract

“…However, it has been shown that iris angiography using indocyanine green dye can show vessels in normal pigmented iris. Recently [85] optical coherence tomography angiography has been advocated for angiography but there are several artifacts in it. On fluorescein iris angiography, the iris vessels are outlined only in blue or green eyes.…”

Section: Normal Human Iris Vascular Pattern On Fluorescein Angiographymentioning

confidence: 99%

“…My study [83] supports the observations of Chignell and Easty [86] that the iris circulation is much slower than the retinal circulation. Normally, the choroidal circulation is much faster than the retinal because of the much larger size of the lumen of the choriocapillaris (up to 50 µ) than of the retinal capillaries (3.5 to 6 µ [85,87]). Thus, of the three visible ocular circulatory systems -choroidal, retinal, and iris-the iris is the slowest.…”

Section: Normal Human Iris Vascular Pattern On Fluorescein Angiographymentioning

confidence: 99%

Uveal vascular bed in health and disease: uveal vascular bed anatomy. Paper 1 of 2

Hayreh

Hayreh²

2023

Eye

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The uveal vascular bed is the largest vascular system in the eye and has a role in supplying almost every tissue in the eyeball. This makes it the most important ocular vascular system. This is an up-to-date review of the literature of the entire uveal vascular bed in health based on detailed anatomy of the posterior ciliary arteries (PCAs), anterior ciliary arteries, cilioretinal arteries, and vortex veins. Although postmortem injection cast preparations gave us useful information on the morphology of the choroidal vascular bed; in vivo studies showed that they misled us for centuries about the in vivo situation. According to the postmortem cast studies, the uveal vascular bed has no segmental distribution, the uveal vessels anastomose freely with one another, there are inter-arterial and arteriovenous anastomoses in the choroid, and the choriocapillaris form a freely communicating and an uninterrupted vascular bed in the entire choroid.

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“…However, most of these traditional RNA-tag probes are stubbornly based on fluorescence antibody immunoadsorption, fluorescein tagging, or isotopic labeling, which need complex operation steps to realize imaging; therefore, these may remain difficult to be analyzed by lagged laboratories . In daily use, the fluorescein-labeled RNA dyes are prone to fluorescence quenching and spectral interference after dyeing due to their limitations; to some extent, the clinical application value of these dyes is limited. , Although the combination of dot-based biological imaging with amplification techniques makes it accessible to identify the 22–23 nt RNA at a single-nanoparticle level with the detection limit of an attomolar scale, the problem of detection sensitivity still cannot be avoided. For example, the single/bis-direct label detection method shows only a difference in the intensity of the transmitted signal at the laboratory level, which makes it difficult to achieve real practical clinical applications .…”

Section: Introductionmentioning

confidence: 99%

Monitoring Clinical–Pathological Grading of Hepatocellular Carcinoma Using MicroRNA-Guided Semiconducting Polymer Dots

Zhang

Lin

et al. 2022

ACS Appl. Mater. Interfaces

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MicroRNAs (miRNAs) are a class of small, noncoding RNAs involved in nearly all genetic central dogma processes and human biological behavior, which also play a significant role in the pathological activity of tumors, such as gene transcription, protein translation, and exosome secretion. Therefore, through the navigation of certain specific miRNAs, we can trace the specific physiological processes or image some specific tissues. Designing and accurately positioning microRNA (miRNA)-sensitive fluorescent nanoprobes with benign specificity and recognition in cells or tissues are a challenging research field. To solve the difficulties, we introduce four semiconducting polymer dots (Pdots) as nanoprobes linked by specific miRNA antisense sequences for monitoring the pathological grading by the variation in miRNA expression. Based on the base pairing principle, these miRNAsensitive Pdots could bind to specific miRNAs within the cancerous cells. As impacted by the background of different pathology gradings, the proportions of the four hepatocellular carcinoma (HCC)-specific miRNAs within the cancerous cell are different, and the pathological grading of the patient tissues can be determined by comparing the palette combinations. The short single-stranded RNA-functionalized Pdots, which have excellent microRNA sensitivity, are observed in an experimental cell model and a series of tissue specimens from HCC patients for the first time. Using the Forster (or fluorescence) resonance energy transfer (FRET) model of Pdots and Cy3dt tag to simulate in vivo miRNA detection, the superior sensitivity and specificity of these nanoprobes are verified. The interference of subjective factors in traditional single/bis-dye emission intensity detection is abandoned, and multiple label staining is used to enhance sensitivity further and reduce the false-positive rate. The feasibility exhibited by this novel staining method is verified in normal hepatocellular HCC cell lines and 16 frozen ultrathin tissue sections, which are employed to quantify pathological grading-related color presentation systems for clinical doctors and pathologists' use. The intelligently designed miRNAguided Pdots will emerge as an ideal platform with promising biological imaging.

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Imaging of iris vasculature: current limitations and future perspective

Cited by 13 publications

References 73 publications

“Shin in the eye”: Au-CS@FNDs as contact lens additives for blocking UV irradiation, bacterial keratitis, and corneal neovascularization therapies

“Shin in the eye”: Au-CS@FNDs as contact lens additives for blocking UV irradiation, bacterial keratitis, and corneal neovascularization therapies

Uveal vascular bed in health and disease: uveal vascular bed anatomy. Paper 1 of 2

Monitoring Clinical–Pathological Grading of Hepatocellular Carcinoma Using MicroRNA-Guided Semiconducting Polymer Dots

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