Imaging of intracellular spherical lamellar structures and tissue gross morphology by a focused ion beam/scanning electron microscope (FIB/SEM)

Imaging of Cell‐to‐Material Interfaces by SEM after in situ Focused Ion Beam Milling on Flat Surfaces and Complex 3D‐Fibrous Structures

“…[20,32] Both groups used slightly different sample preparation techniques: critical point-dried and freeze-dried, respectively, but obtained very similar image quality and detail structures. In particular, when cellto-substrate interfaces of cell monolayers cultured on glass or metal surface were analyzed, surface damage due to uncontrollable Gallium-ion implementation, amorphization, material deposition, melting of material ( [29] and references therein) could be neglected whereas the so called ''curtain effect'' was observable (here: especially in Fig. 3b and 4f).…”

“…[24,25] In order to analyze the 3D-orientation of digestive gland epithelium of a terrestrial crustacean chemically fixed and critical point dried samples were used. [26][27][28][29][30][31] All previous studies aimed at investigating purely biological materials however sample preparation for direct visualization of the cell-to-material interface needs to be optimized. Very few studies address this topic using critical point-dried or freeze-dried cells on flat surfaces.…”

Focused ion beam (FIB) combined with high resolution scanning electron microscopy: A promising tool for 3D analysis of chromosome architecture

“…Focused ion beam (FIB) milling, sequential removal of material slices, is beginning to be established in biosciences (Drobne et al, 2007;Knott et al, 2008), allowing serial sectioning of biological specimens in dimensions virtually unachievable until now. This investigation applied combined FIB and FESEM in a high resolution two-beam FIB/FESEM system with fixed and critical point dried whole mount chromosome preparations to address the following questions: How compact is the chromosome interior?…”

Chromosome Centromeres: Structural and Analytical Investigations with High Resolution Scanning Electron Microscopy in Combination with Focused Ion Beam Milling

“…Recently, distributions of 3 histone H3 variants have been compared with FESEM in barley chromosomes, revealing centromere-specific patterns for unmodified histone H3, H3S10ph, and CENH3 located to chromosome substructures [Schroeder-Reiter et al, 2003;Houben et al, 2007]. FESEM in combination with focused ion beam (FIB) applications are relatively new for biological studies [Drobne et al, 2007;Knott et al, 2008] but present a promising possibility for getting insight into biological substructures. The present study aims to structurally characterize centromere variants to determine whether there are conserved centromere structures (i.e., parallel fibrils) for a variety of organisms.…”