Imaging of intracellular protein aggregates through plasmon-assisted clusteroluminescence

Dhillon, Ashish Kumar; Dudhe, Pranay Eknath; Majumdar, Shubhangi; Barman, Sanmitra; Ghosh, Dibyajyoti; Dhanasekaran, Karthigeyan; Siddhanta, Soumik

doi:10.1039/d4nr01803f

Nanoscale

2024

DOI: 10.1039/d4nr01803f

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Imaging of intracellular protein aggregates through plasmon-assisted clusteroluminescence

Ashish Kumar Dhillon,

Pranay Eknath Dudhe,

Shubhangi Majumdar

et al.

Abstract: The formation of clusters in non-aromatic molecules can give rise to unconventional luminescence or clusteroluminescence. Typically containing heteroatoms without extended conjugation or aromatic rings, these molecules have drawn much attention...

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Photoinduced Electron-Transfer-Mediated Differential Recognition of Proteins on Plasmonic Surfaces

Dhillon,

Barman,

Siddhanta

2024

ACS Appl. Mater. Interfaces

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Photoinduced enhanced Raman spectroscopy (PIERS) has emerged as an efficient technique for enhancing the vibrational modes of analyte molecules adsorbed on a plasmonic nanoparticle-semiconductor hybrid material through chemical enhancement governed by electron transfer from the semiconductor to the plasmonic nanoparticles under an additional ultraviolet (UV) preirradiation step. The increase in chemical enhancement is imperative in analyzing and detecting pharmaceutically important moieties, such as amino acids and proteins, with a low Raman scattering cross section, even in complex biological environments. Herein, we demonstrate that UV preirradiation induced the creation of additional oxygen vacancies by introducing a low concentration (≈1%) of Ni as a dopant in the 2D platelike morphology of the BiOCl semiconductor; i.e., defect states in the semiconductor can induce charge transfer from the semiconductor to the plasmonic nanoparticles. This phenomenon facilitates electron transfer to the adsorbed analyte on the plasmonic surface. Additionally, we have shown the usefulness of this method in protein immobilization on the substrate surface, followed by the identification of a specific protein in the mixture of proteins. Proteins containing cysteine residues capture these electrons to form a surface-bound thiol group via a transient disulfide electron adduct radical. This allows differential binding of the protein molecules to the semiconductor plasmonic hybrid depending on the concentration of surface cysteine residues in proteins. Through PIERS and principal component analysis, we demonstrate the possibility of probing and distinguishing biomolecules based on their surface composition and secondary structure components even in their mixtures, thus paving the way for efficient analysis of complex biological systems.

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Photoinduced Electron-Transfer-Mediated Differential Recognition of Proteins on Plasmonic Surfaces

Dhillon,

Barman,

Siddhanta

2024

ACS Appl. Mater. Interfaces

View full text Add to dashboard Cite

show abstract

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Imaging of intracellular protein aggregates through plasmon-assisted clusteroluminescence

Abstract: The formation of clusters in non-aromatic molecules can give rise to unconventional luminescence or clusteroluminescence. Typically containing heteroatoms without extended conjugation or aromatic rings, these molecules have drawn much attention...

Cited by 1 publication

References 61 publications

Photoinduced Electron-Transfer-Mediated Differential Recognition of Proteins on Plasmonic Surfaces

Photoinduced Electron-Transfer-Mediated Differential Recognition of Proteins on Plasmonic Surfaces

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