Imaging of human glioblastoma cells and their interactions with mesenchymal stem cells in the zebrafish <i>(Danio rerio)</i> embryonic brain

Vittori, Miloš; Breznik, Barbara; Gredar, Tajda; Hrovat, Katja; Mali, Lilijana Bizjak; Lah, Tamara T.

doi:10.1515/raon-2016-0017

Cited by 19 publications

(26 citation statements)

References 47 publications

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“…The most common, currently used systems are based on immunodeficient rodent lines, which provide a combination of an organism-level model, fluorescent microscopy, FACS analysis of cell subpopulations, and transcription analysis [ 48 ]. However, the D. rerio model is gaining in popularity, especially where high-resolution microscopy is warranted [ 49 , 50 ]. It is very likely that implementation of these approaches, in combination with a syngeneic transplantation model based on optically transparent D. rerio lines, will provide fundamentally new insight into understanding tumor growth and its interaction with its microenvironment.…”

Section: Prospects Of Model Developmentmentioning

confidence: 99%

Organism-Level Tumor Models in Zebrafish Danio rerio

et al. 2018

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Development and implementation of adequate organism-level models is one of the key elements in biomedical research that focuses on experimental oncology. Over the last decade, studies using Zebrafish (Danio rerio) have gained in popularity in this area of research. This review describes the various approaches that have been used in developing highly effective models for oncological (clinical term, better cancer or tumor) studies based on D. rerio. Priority is given to transplantation models of cancer and their application to optically transparent D. rerio lines, including clonal ones, and utilization tumors of various origins bearing fluorescent labels. The combination of tumor transplantation at organism-level models in transparent clonal D. rerio lines with fluorescent microscopy, FACS-fractionation of tumor cell subsets, and transcription analysis can result in one of the most promising research approaches in providing new information on tumor formation and growth.

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Section: Prospects Of Model Developmentmentioning

confidence: 99%

Organism-Level Tumor Models in Zebrafish Danio rerio

et al. 2018

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“…Implanted cells were able to initiate tumors showing stemness properties. Moreover, experiments of co-transplantation of mesenchymal stem cells (MSCs) and U87/U373 showed a contribution of MSCs to GBM tumoral heterogeneity and tumor invasion [ 130 , 131 ].…”

Section: Human Glioma and Relevant Target Pathwaysmentioning

confidence: 99%

Zebrafish in Translational Cancer Research: Insight into Leukemia, Melanoma, Glioma and Endocrine Tumor Biology

Idilli

Precazzini

Mione

et al. 2017

Genes

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Over the past 15 years, zebrafish have emerged as a powerful tool for studying human cancers. Transgenic techniques have been employed to model different types of tumors, including leukemia, melanoma, glioblastoma and endocrine tumors. These models present histopathological and molecular conservation with their human cancer counterparts and have been fundamental for understanding mechanisms of tumor initiation and progression. Moreover, xenotransplantation of human cancer cells in embryos or adult zebrafish offers the advantage of studying the behavior of human cancer cells in a live organism. Chemical-genetic screens using zebrafish embryos have uncovered novel druggable pathways and new therapeutic strategies, some of which are now tested in clinical trials. In this review, we will report on recent advances in using zebrafish as a model in cancer studies—with specific focus on four cancer types—where zebrafish has contributed to novel discoveries or approaches to novel therapies.

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“…Their transparency and internal physiological similarities to humans 1,2 make them ideal models for a variety of research areas, including immunological and cancer development studies. 2,3 There are numerous wild-type, mutant, and transgenic zebrafish lines in existence, and the NIH-supported Zebrafish International Resource Center (ZIRC, Eugene, OR) is a principal supplier of such lines.…”

Section: Introductionmentioning

confidence: 99%

Survival of Bacterial and Parasitic Pathogens from Zebrafish (Danio rerio) After Cryopreservation and Thawing

2018

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Cryopreservation is a common method used to preserve the sperm of various animal species, and it is widely used with zebrafish (Danio rerio). As with other animals, there is a possibility of paternal pathogen transmission through sperm. We evaluated the ability of five common and important pathogens of zebrafish to survive cryopreservation as used with zebrafish sperm and freezing without cryopreservant. We evaluated Mycobacterium chelonae, Mycobacterium marinum, and Edwardsiella ictaluri, each originally isolated from zebrafish, eggs of Pseuodocapillaria tomentosa, and spores of Pseudoloma neurophilia. Each mycobacterial isolate showed relatively minimal reduction in survival after freezing and thawing, particularly when subjected to cryopreservation. E. ictaluri also showed survival after cryopreservation, but exhibited a several log reduction after freezing at -80°C without cryopreservant. With P. neurophilia, two separate experiments conducted 3 years apart yielded very similar results, showing some, but reduced, survival of spores by using three different viability assays: SYTOX stain, Fungi-Fluor stain, and presence of a spore vacuole. Eggs of P. tomentosa showed no survival based on larvation of eggs when subjected to either freezing method. Given that four of the five pathogens exhibited survival after cryopreservation, we recommend that sperm samples or donor male zebrafish fish be tested for pathogens when sperm are to be stored by using cryopreservation.

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Imaging of human glioblastoma cells and their interactions with mesenchymal stem cells in the zebrafish (Danio rerio) embryonic brain

Cited by 19 publications

References 47 publications

Organism-Level Tumor Models in Zebrafish Danio rerio

Organism-Level Tumor Models in Zebrafish Danio rerio

Zebrafish in Translational Cancer Research: Insight into Leukemia, Melanoma, Glioma and Endocrine Tumor Biology

Survival of Bacterial and Parasitic Pathogens from Zebrafish (Danio rerio) After Cryopreservation and Thawing

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