Imaging of cerebrospinal fluid space and movement of hydrocephalus mice using near infrared fluorescence

Shibata, Yasushi; Kruskal, Jonathan B.; Palmer, Matthew R.

doi:10.1007/s10072-007-0792-2

Cited by 5 publications

(3 citation statements)

References 21 publications

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“…For example, computer tomography cannot operate at single cell level due to low spatial and temporal resolution [16, 19]. The intravital fluorescent microscopy has been applied for imaging fluid itself (so-called, cisternography), but not single biomarkers in CSF [20]. Photoacoustic flow cytometry (PAFC) based on detection of laser-induced acoustic waves, which is not sensitive to light scattering or autofluorescence, offers advanced alternatives for diagnosis diseases at single cell level in vivo including detection of CTCs and leukocytes in blood and lymph flows as well as bacteria in circulating blood [21–22].…”

Section: Introductionmentioning

confidence: 99%

Photoacoustic and photothermal detection of circulating tumor cells, bacteria and nanoparticles in cerebrospinal fluid in vivo and ex vivo

Nedosekin

Juratli

Sarimollaoglu

et al. 2013

Journal of Biophotonics

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Circulating cells, proteins, microparticles, and DNA in cerebrospinal fluid (CSF) are excellent biomarkers of many diseases, including cancer and infections. However, the sensitivity of existing methods is limited in their ability to detect rare CSF biomarkers at the treatable, early-stage of diseases. Here, we introduce novel CSF tests based on in vivo multicolor photoacoustic flow cytometry (PAFC) and ex vivo photothermal scanning cytometry. In the CSF of tumor-bearing mice, we molecularly detected in vivo circulating tumor cells (CTCs) before the development of breast cancer brain metastasis with 20-times higher sensitivity than with current assays. For the first time, we demonstrated assessing three pathways (i.e., blood, lymphatic, and CSF) of CTC dissemination, tracking nanoparticles in CSF in vivo and their imaging ex vivo. In label-free CSF samples, we counted leukocytes, erythrocytes, melanoma cells, and bacteria and imaged intracellular cytochromes, hemoglobin, melanin, and carotenoids, respectively. Taking into account the safety of PAFC, its translation for use in humans is expected to improve disease diagnosis beyond conventional detection limits.

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Section: Introductionmentioning

confidence: 99%

Photoacoustic and photothermal detection of circulating tumor cells, bacteria and nanoparticles in cerebrospinal fluid in vivo and ex vivo

Nedosekin

Juratli

Sarimollaoglu

et al. 2013

Journal of Biophotonics

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“…The use of CT is required; however, ongoing investigations of low-cost, noninvasive lateral ventricular imaging may serve as a valuable addition to our proposed navigation system to provide reference imaging for EVD placement. 40,41…”

Section: Future Directionsmentioning

confidence: 99%

Robotic external ventricular drain placement for acute neurosurgical care in low-resource settings: feasibility considerations and a prototype design

Smith

Teague

Naik

et al. 2022

Neurosurgical Focus

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OBJECTIVE Emergency neurosurgical care in lower-middle-income countries faces pronounced shortages in neurosurgical personnel and infrastructure. In instances of traumatic brain injury (TBI), hydrocephalus, and subarachnoid hemorrhage, the timely placement of external ventricular drains (EVDs) strongly dictates prognosis and can provide necessary stabilization before transfer to a higher-level center of care that has access to neurosurgery. Accordingly, the authors have developed an inexpensive and portable robotic navigation tool to allow surgeons who do not have explicit neurosurgical training to place EVDs. In this article, the authors aimed to highlight income disparities in neurosurgical care, evaluate access to CT imaging around the world, and introduce a novel, inexpensive robotic navigation tool for EVD placement. METHODS By combining the worldwide distribution of neurosurgeons, CT scanners, and gross domestic product with the incidence of TBI, meningitis, and hydrocephalus, the authors identified regions and countries where development of an inexpensive, passive robotic navigation system would be most beneficial and feasible. A prototype of the robotic navigation system was constructed using encoders, 3D-printed components, machined parts, and a printed circuit board. RESULTS Global analysis showed Montenegro, Antigua and Barbuda, and Seychelles to be primary candidates for implementation and feasibility testing of the novel robotic navigation system. To validate the feasibility of the system for further development, its performance was analyzed through an accuracy study resulting in accuracy and repeatability within 1.53 ± 2.50 mm (mean ± 2 × SD, 95% CI). CONCLUSIONS By considering regions of the world that have a shortage of neurosurgeons and a high incidence of EVD placement, the authors were able to provide an analysis of where to prioritize the development of a robotic navigation system. Subsequently, a proof-of-principle prototype has been provided, with sufficient accuracy to target the ventricles for EVD placement.

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“…New generations of MRI, such as phase-contrast MRI, enable quantitative measurements of CSF flow but not suitable for detection of relatively fast moving single CTCs and particles due to slow time response [75]. The same limitation applies to intravital fluorescence microscopy which has been used for imaging CSF plasma (so-called, cisternography) but not single cells in CSF [76]. Furthermore, the translation of fluorescent neuroimaging to humans in vivo is problematic due to (1) cytotoxicity of fluorophores, (2) undesirable immune responses to tags and (3) assessing only superficial fluid flows due to strong influence of autofluorescent and scattering background.…”

Section: In Vivo Diagnosis Of Csfmentioning

confidence: 99%

New Frontiers in Diagnosis and Therapy of Circulating Tumor Markers in Cerebrospinal Fluid In Vitro and In Vivo

Sindeeva

Verkhovskii

Sarimollaoglu

et al. 2019

Cells

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One of the greatest challenges in neuro-oncology is diagnosis and therapy (theranostics) of leptomeningeal metastasis (LM), brain metastasis (BM) and brain tumors (BT), which are associated with poor prognosis in patients. Retrospective analyses suggest that cerebrospinal fluid (CSF) is one of the promising diagnostic targets because CSF passes through central nervous system, harvests tumor-related markers from brain tissue and, then, delivers them into peripheral parts of the human body where CSF can be sampled using minimally invasive and routine clinical procedure. However, limited sensitivity of the established clinical diagnostic cytology in vitro and MRI in vivo together with minimal therapeutic options do not provide patient care at early, potentially treatable, stages of LM, BM and BT. Novel technologies are in demand. This review outlines the advantages, limitations and clinical utility of emerging liquid biopsy in vitro and photoacoustic flow cytometry (PAFC) in vivo for assessment of CSF markers including circulating tumor cells (CTCs), circulating tumor DNA (ctDNA), microRNA (miRNA), proteins, exosomes and emboli. The integration of in vitro and in vivo methods, PAFC-guided theranostics of single CTCs and targeted drug delivery are discussed as future perspectives.

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Imaging of cerebrospinal fluid space and movement of hydrocephalus mice using near infrared fluorescence

Cited by 5 publications

References 21 publications

Photoacoustic and photothermal detection of circulating tumor cells, bacteria and nanoparticles in cerebrospinal fluid in vivo and ex vivo

Photoacoustic and photothermal detection of circulating tumor cells, bacteria and nanoparticles in cerebrospinal fluid in vivo and ex vivo

Robotic external ventricular drain placement for acute neurosurgical care in low-resource settings: feasibility considerations and a prototype design

New Frontiers in Diagnosis and Therapy of Circulating Tumor Markers in Cerebrospinal Fluid In Vitro and In Vivo

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