Imaging of bone tumors using a monoclonal antibody raised against human osteosarcoma

Armitage, N. C.; Perkins, Alan C.; Pimm, M. V.; Wastie, M. L.; Hopkins, J. Stephen; Dowling, Francis; Baldwin, Robert; Hardcastle, J. D.

doi:10.1002/1097-0142(19860701)58:1<37::aid-cncr2820580108>3.0.co;2-4

Cited by 13 publications

(1 citation statement)

References 14 publications

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“…Many studies over the last 10 years have demonstrated that radiolabelled tumour associated antibodies may be successfully used for the imaging of a variety of human cancers including those of the colon (Chatal et al, 1984), ovary (Shepherd et al, 1987), breast (Rainsbury et al, 1983), lung (Chan et al, 1986), skin (Carrasquillo et al, 1987) and bone (Armitage et al, 1986). These studies have reported the results of imaging but have not attempted to correlate the results with the biodistribution of the radiolabelled antibody.…”

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The effect of circulating antigen and radiolabel stability on the biodistribution of an indium labelled antibody

Davidson

Babich²,

Young³

et al. 1991

Br J Cancer

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Summary This study has investigated two of the main problems with radiolabelled antibody imaging, the formation of circulating immune complexes (I.C.) and the non specific binding of radiolabel to the antibody molecule.Patients undergoing immunoscintigraphy with "'In labelled monoclonal antibody ICR2 were divided into three groups who received either the radiolabelled antibody alone (control, n = 12), the radiolabelled antibody which was incubated with the chelating agent diethylene triamine pentacetic acid (DTPA) prior to size exclusion chromatography (n = 6) or whose injectate was treated with DTPA and cold MAb administered intravenously prior to radiolabelled MAb administration (n = 6). Radiolabelled antibody uptake in abdominal organs was measured by region of interest analysis using a gamma camera with online computer and that in tumour and normal tissues by gamma well counting of biopsies. Circulating antigen and immune complex was measured by high pressure liquid chromatography (HPLC).The sensitivity of tumour imaging and the tumour uptake of radiolabelled antibody was not significantly different between the groups. Patients with high circulating antigen levels developed high levels of circulating immune complex but also had high tumour uptakes of radiolabelled antibody. Administration of cold MAb increased the splenic, but did not effect the tumour uptake of radiolabelled antibody and only minimally reduced levels of circulating immune complex. Chelate administration reduced the urinary excretion of radioactivity but increased the liver uptake of radioactivity.These results have shown that successful antibody imaging can be carried out despite high levels of circulating antigen, that large doses of unlabelled antibody are required to prevent immune complex formation and that removal of non specifically bound "'In does not reduce the liver uptake of radioactivity.

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confidence: 99%

The effect of circulating antigen and radiolabel stability on the biodistribution of an indium labelled antibody

Davidson

Babich²,

Young³

et al. 1991

Br J Cancer

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Imaging of a parapharyngeal hemangiopericytoma. Radioimmunoscintigraphy (SPECT) with indium-111-labeled anti-cea antibody, and comparison to digital subtraction angiography, computed tomography, and immunohistochemistry

Kairemo

Hopsu

Melartin

et al. 1991

Cancer

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A 27-year-old male patient with a parapharyngeal hemangiopericytoma was investigated radiologically with orthopantomography, computed tomography, and digital subtraction angiography before the operation. Because a malignancy was suspected, the patient was imaged with gamma camera using radiolabeled monoclonal anticarcinoembryonal antigen antibody including single photon emission computed tomography. The radioantibody accumulated strongly into the neoplasm. Tumor to background ratio was 2.2. Samples of the excised tumor were stained immunohistochemically for desmin, vimentin, muscle actin, cytokeratin, CEA (carcinoembryonic antigen), and factor VIII. They showed that the antibody uptake was of unspecific nature and not due to CEA expression in the tumor.

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Anti-epithelial membrane antigen monoclonal antibodies and radioimmunolocalization of colorectal cancer

Yiu

Baker

Boulos

1991

Journal of British Surgery

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The monoclonal antibodies LICR-LON M8 and 77-1, which react with epithelial membrane antigen, showed a strong reaction with colorectal cancer on immunohistochemistry. In a radioimmunolocalization study in patients with colorectal cancer, 111In-labelled M8 detected 13 of 16 tumour sites present in 16 patients. 111In-labelled 77-1 detected 10 of 15 tumour sites present in 14 patients. The high radioactive background in the liver prevented the detection of hepatic metastases in eight patients (five in the M8 and three in the 77-1 group). The mean (s.d.) tumour to normal colon ratio was 2.5(1.2) for M8 and 1.6(0.5) for 77-1 at day 6 after antibody administration. The mean (s.d.) tumour to blood ratio was 4.9(3.7) for M8 and 3.6(1.5) for 77-1. There was no statistical difference between these results. All scan-positive cancers reacted with M8 and 77-1 on immunohistochemistry. The results suggest that these monoclonal antibodies may have a role in the immunolocalization of colorectal cancer.

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Imaging of bone tumors using a monoclonal antibody raised against human osteosarcoma

Cited by 13 publications

References 14 publications

The effect of circulating antigen and radiolabel stability on the biodistribution of an indium labelled antibody

The effect of circulating antigen and radiolabel stability on the biodistribution of an indium labelled antibody

Imaging of a parapharyngeal hemangiopericytoma. Radioimmunoscintigraphy (SPECT) with indium-111-labeled anti-cea antibody, and comparison to digital subtraction angiography, computed tomography, and immunohistochemistry

Anti-epithelial membrane antigen monoclonal antibodies and radioimmunolocalization of colorectal cancer

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