Imaging Molecular Adhesion in Cell Rolling by Adhesion Footprint Assay

Altering Cell Junctional Tension in Spheroids through E-Cadherin Engagement Modulation

“…According to our previous work, Protein G was conjugated to the amine-modified DNA strand (backbone-ligand) using a bifunctional sulfo-SMCC linker . Briefly, Protein G, Sulfo-SMCC, and amine-DNA were separately reconstituted in Ultrapure water.…”

“…Protein G-DNA was purified using his-tag isolation with Dynabeads. The Protein G-DNA concentration was quantified using a custom-written script in MATLAB (Mathworks) . Each strand was mixed to assemble unzipping and shearing TGT at the same molar ratio.…”

“…According to our previous work, Protein G was conjugated to the amine-modified DNA strand (backbone-ligand) using a bifunctional sulfo-SMCC linker. 32 Briefly, Protein G, Sulfo-SMCC, and amine-DNA were separately reconstituted in Ultrapure water. Twenty μL of 8 mg/mL Protein G (5 nmol) was reduced by incubation with 4 μL of tris(2-carboxyethyl)phosphine (TCEP) solution (120 mM TCEP, 50 mM and EDTA, 1× PBS) for 30 min at room temperature.…”

“…The Protein G-DNA concentration was quantified using a custom-written script in MATLAB (Mathworks). 32 Each strand was mixed to assemble unzipping and shearing TGT at the same molar ratio. The mixture of TGT was incubated in 1× PBS containing 1 mM MgCl 2 overnight at 4 °C.…”