Imaging mass spectrometry reveals complex lipid distributions across Staphylococcus aureus biofilm layers

Rivera, Emilio; Weiss, Amy L.; Migas, Lukasz; Freiberg, Jeffrey A.; Neumann, Elizabeth K.; Plas, Raf Van de; Spraggins, Jeffrey M.; Skaar, Eric P.; Caprioli, Richard M.

doi:10.1016/j.jmsacl.2022.09.003

Cited by 14 publications

(16 citation statements)

References 78 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…For instance, a vertical gradient of viscoelasticity is established during the preliminary stages of S. aureus biofilm development, which also facilitates biofilm dispersal, removing loosely bound bacteria while retaining an entrenched layer in the biofilm structure [ 31 ]. Rivera et al revealed stark lipidomic heterogeneity in S. aureus biofilm, demonstrating that each horizontal layer was molecularly distinct [ 32 ]. Future applications of these findings to study spatially localized molecular responses to antibiofilm agents could provide new therapeutic strategies.…”

Section: Formation and Properties Of S Aureus Biofilmmentioning

confidence: 99%

Staphylococcus aureus biofilm: Formulation, regulatory, and emerging natural products-derived therapeutics

Wu,

Wang,

Xiong

et al. 2024

Biofilm

View full text Add to dashboard Cite

Section: Formation and Properties Of S Aureus Biofilmmentioning

confidence: 99%

Staphylococcus aureus biofilm: Formulation, regulatory, and emerging natural products-derived therapeutics

Wu,

Wang,

Xiong

et al. 2024

Biofilm

View full text Add to dashboard Cite

“…Proteomics has also been coupled with imaging analysis to investigate the proteome spatially within the biofilm. One of the most utilised methods of achieving this is with MALDI-ToF imaging mass spectrometry (MALDI-ToF IMS) ( Floyd et al., 2015 ; Rivera et al., 2022 ), whereby biofilms are cryo-sectioned and imaged with a fluorescent microscope prior to MALDI-ToF ( Rivera et al., 2022 ) ( Figure 3B ) or biofilms are grown on slides and subjected to electron microscopy and MALDI-ToF ( Floyd et al., 2015 ). Another method is laser ablation sample transfer (LAST), where biofilms can be grown on transwell membranes which are then laid on a microscope slide on the anoxic or oxic side and subjected to LC-MS/MS ( Pulukkody et al., 2021 ).…”

Section: Genetic and Molecular Analysis Techniquesmentioning

confidence: 99%

How to study biofilms: technological advancements in clinical biofilm research

Cleaver,

Garnett

2023

Front. Cell. Infect. Microbiol.

View full text Add to dashboard Cite

Biofilm formation is an important survival strategy commonly used by bacteria and fungi, which are embedded in a protective extracellular matrix of organic polymers. They are ubiquitous in nature, including humans and other animals, and they can be surface- and non-surface-associated, making them capable of growing in and on many different parts of the body. Biofilms are also complex, forming polymicrobial communities that are difficult to eradicate due to their unique growth dynamics, and clinical infections associated with biofilms are a huge burden in the healthcare setting, as they are often difficult to diagnose and to treat. Our understanding of biofilm formation and development is a fast-paced and important research focus. This review aims to describe the advancements in clinical biofilm research, including both in vitro and in vivo biofilm models, imaging techniques and techniques to analyse the biological functions of the biofilm.

show abstract

“…Besides the possibility of ion suppression from the paraffin, lipids can form a cross-link with formalin, causing class-specific depletion of lipid ion signals in formalin-fixed tissues. − Therefore, fresh-frozen material is preferred for MALDI-MSI. Other traditional embedding compounds such as optimal cutting temperature (OCT) matrix are known to interfere with MALDI-MSI experiments . Recently, a study demonstrated the use of Epredia M-1 embedding matrix (M-1), a cellulose-based embedding medium, to be a suitable substitute for embedding specimens with high water content, such as eyeballs .…”

Section: Introductionmentioning

confidence: 99%

“…Other traditional embedding compounds such as optimal cutting temperature (OCT) matrix are known to interfere with MALDI-MSI experiments. 25 Recently, a study demonstrated the use of Epredia M-1 embedding matrix (M-1), a cellulose-based embedding medium, to be a suitable substitute for embedding specimens with high water content, such as eyeballs. 26 Compounds such as M-1 offer exciting prospects for embedding and imaging organoids because of their user-friendly characteristics.…”

Section: Introductionmentioning

confidence: 99%

Lipidomic Phenotyping Of Human Small Intestinal Organoids Using Matrix-Assisted Laser Desorption/Ionization Mass Spectrometry Imaging

Duivenvoorden,

Claes,

van der Vloet

et al. 2023

Anal. Chem.

View full text Add to dashboard Cite

In the past decade, interest in organoids for biomedical research has surged, resulting in a higher demand for advanced imaging techniques. Traditional specimen embedding methods pose challenges, such as analyte delocalization and histological assessment. Here, we present an optimized sample preparation approach utilizing an Epredia M-1 cellulose-based embedding matrix, which preserves the structural integrity of fragile small intestinal organoids (SIOs). Additionally, background interference (delocalization of analytes, nonspecific (histological) staining, matrix ion clusters) was minimized, and we demonstrate the compatibility with matrix-assisted laser desorption/ionization mass spectrometry imaging (MALDI-MSI). With our approach, we can conduct label-free lipid imaging at the single-cell level, thereby yielding insights into the spatial distribution of lipids in both positive and negative ion modes. Moreover, M-1 embedding allows for an improved coregistration with histological and immunohistochemical (IHC) stainings, including MALDI-IHC, facilitating combined untargeted and targeted spatial information. Applying this approach, we successfully phenotyped crypt-like (CL) and villus-like (VL) SIOs, revealing that PE 36:2 [M – H] − ( m / z 742.5) and PI 38:4 [M – H] − ( m / z 885.5) display higher abundance in CL organoids, whereas PI 36:1 [M – H] − ( m / z 863.6) was more prevalent in VL organoids. Our findings demonstrate the utility of M-1 embedding for advancing organoid research and unraveling intricate biological processes within these in vitro models.

show abstract

Imaging mass spectrometry reveals complex lipid distributions across Staphylococcus aureus biofilm layers

Cited by 14 publications

References 78 publications

Staphylococcus aureus biofilm: Formulation, regulatory, and emerging natural products-derived therapeutics

Staphylococcus aureus biofilm: Formulation, regulatory, and emerging natural products-derived therapeutics

How to study biofilms: technological advancements in clinical biofilm research

Lipidomic Phenotyping Of Human Small Intestinal Organoids Using Matrix-Assisted Laser Desorption/Ionization Mass Spectrometry Imaging

Contact Info

Product

Resources

About