Imaging mass spectrometry in biomarker discovery and validation

Schwamborn, Kristina

doi:10.1016/j.jprot.2012.06.015

Cited by 64 publications

(49 citation statements)

References 75 publications

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“…Until now, use of MALDI imaging mass spectrometry (IMS) for identifi cation of candidate biomarkers has primarily focused on the examination of protein patterns (19)(20)(21). However, the identifi cation of proteins corresponding to disease-classifying m/z values, although occasionally accomplished ( 22 ), remains a challenging analytical task, as MALDI-IMS protein signals are typically weak and obtained in low-resolution linear detection mode ( 20 ).…”

Section: Animalsmentioning

confidence: 99%

“…However, the identifi cation of proteins corresponding to disease-classifying m/z values, although occasionally accomplished ( 22 ), remains a challenging analytical task, as MALDI-IMS protein signals are typically weak and obtained in low-resolution linear detection mode ( 20 ). Protein IMS biomarker discovery workfl ows therefore require follow-up with complementary proteomics methods ( 22,23 ) and immunohistochemical validation ( 19,21 ). On the other hand, mounting experimental evidence suggests that GSLs could be useful molecular markers of disease ( 24 ), while at the same time being present in higher concentrations than most proteins and being more readily extracted and analyzed in organic solvents typically used for MALDI-IMS ( 25 ).…”

Section: Animalsmentioning

confidence: 99%

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MALDI imaging MS reveals candidate lipid markers of polycystic kidney disease

Ruh

Salonikios

Fuchser

et al. 2013

Journal of Lipid Research

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Section: Animalsmentioning

confidence: 99%

Section: Animalsmentioning

confidence: 99%

MALDI imaging MS reveals candidate lipid markers of polycystic kidney disease

Ruh

Salonikios

Fuchser

et al. 2013

Journal of Lipid Research

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“…MSI involves the gathering of MS spectra in a spatially defined manner for a particular sample of interest such that the distribution of a large number of biochemicals such as metabolites and proteins can be obtained within a single experiment. The potential of MSI to improve analysis of biological samples has already been demonstrated in many previous studies, such as the ability to image metabolite distributions in mouse brain sections [2], determine tissue type in tumors [3], to image protein localizations in formalin fixed tissues [4], biomarker discovery [5], and many others.…”

Section: Introductionmentioning

confidence: 97%

High Resolution Tissue Imaging Using the Single-probe Mass Spectrometry under Ambient Conditions

Rao

Pan

Yang

2015

J. Am. Soc. Mass Spectrom.

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Abstract. Ambient mass spectrometry imaging (MSI) is an emerging field with great potential for the detailed spatial analysis of biological samples with minimal pretreatment. We have developed a miniaturized sampling and ionization device, the Singleprobe, which uses in-situ surface micro-extraction to achieve high detection sensitivity and spatial resolution during MSI experiments. The Single-probe was coupled to a Thermo LTQ Orbitrap XL mass spectrometer and was able to create high spatial and high mass resolution MS images at 8±2 and 8.5 μm on flat polycarbonate microscope slides and mouse kidney sections, respectively, which are among the highest resolutions available for ambient MSI techniques. Our proof-of-principle experiments indicate that the Single-probe MSI technique has the potential to obtain ambient MS images with very high spatial resolutions with minimal sample preparation, which opens the possibility for subcellular ambient tissue MSI to be performed in the future.

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“…During the MSI analysis, mass spectrometry (MS) measurements are recorded across the surface on an individual pixel basis to create a 2D image of the species of interest 1 . MSI techniques have the ability to provide a spatially resolved feature distribution for a large range of metabolites, allowing a much greater amount of information to be obtained from a sample than from using traditional molecular imaging techniques, and they have the potential to greatly improve the analysis of biological samples for biological and pharmacology studies 2 . MSI can be broadly separated into non-ambient and ambient approaches.…”

Section: Introductionmentioning

confidence: 99%

Applications of the Single-probe: Mass Spectrometry Imaging and Single Cell Analysis under Ambient Conditions

Rao

Pan

Yang

2016

JoVE

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Mass spectrometry imaging (MSI) and in-situ single cell mass spectrometry (SCMS) analysis under ambient conditions are two emerging fields with great potential for the detailed mass spectrometry (MS) analysis of biomolecules from biological samples. The single-probe, a miniaturized device with integrated sampling and ionization capabilities, is capable of performing both ambient MSI and in-situ SCMS analysis. For ambient MSI, the single-probe uses surface micro-extraction to continually conduct MS analysis of the sample, and this technique allows the creation of MS images with high spatial resolution (8.5 μm) from biological samples such as mouse brain and kidney sections. Ambient MSI has the advantage that little to no sample preparation is needed before the analysis, which reduces the amount of potential artifacts present in data acquisition and allows a more representative analysis of the sample to be acquired. For in-situ SCMS, the single-probe tip can be directly inserted into live eukaryotic cells such as HeLa cells, due to the small sampling tip size (< 10 μm), and this technique is capable of detecting a wide range of metabolites inside individual cells at near real-time. SCMS enables a greater sensitivity and accuracy of chemical information to be acquired at the single cell level, which could improve our understanding of biological processes at a more fundamental level than previously possible. The single-probe device can be potentially coupled with a variety of mass spectrometers for broad ranges of MSI and SCMS studies.

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Imaging mass spectrometry in biomarker discovery and validation

Cited by 64 publications

References 75 publications

MALDI imaging MS reveals candidate lipid markers of polycystic kidney disease

MALDI imaging MS reveals candidate lipid markers of polycystic kidney disease

High Resolution Tissue Imaging Using the Single-probe Mass Spectrometry under Ambient Conditions

Applications of the Single-probe: Mass Spectrometry Imaging and Single Cell Analysis under Ambient Conditions

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