Imaging Mass Spectrometric Analysis of Neurotransmitters

Romero-Pérez, Gustavo A.; Takei, Shiro; Yao, Ikuko

doi:10.5702/massspectrometry.s0049

Cited by 7 publications

(4 citation statements)

References 65 publications

(95 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Here, we demonstrate the existence of sulfatide at the prooligodendroblast stage. We also show that the sulfatide fatty acid chain length changes during oligodendrocyte development, using an imaging mass spectrometry (IMS) technique, which enables the simultaneous visualization of the spatial distribution of numerous biomolecules (Romero-Perez et al 2014). We initially utilize IMS with a matrix-assisted laser ionization (MALDI) system for a developmental analysis.…”

mentioning

confidence: 99%

Sulfatide species with various fatty acid chains in oligodendrocytes at different developmental stages determined by imaging mass spectrometry

Hirahara

Wakabayashi

Mori

et al. 2016

Journal of Neurochemistry

Self Cite

View full text Add to dashboard Cite

HSO3-3-galactosylceramide (Sulfatide) species comprise the major glycosphingolipid components of oligodendrocytes and myelin and play functional roles in the regulation of oligodendrocyte maturation and myelin formation. Although various sulfatide species contain different fatty acids, it is unclear how these sulfatide species affect oligodendrogenesis and myelination. The O4 monoclonal antibody reaction with sulfatide has been widely used as a useful marker for oligodendrocytes and myelin. However, sulfatide synthesis during the pro-oligodendroblast stage, where differentiation into the oligodendrocyte lineage has already occurred, has not been examined. Notably, this stage comprises O4-positive cells. In this study, we identified a sulfatide species from the pro-oligodendroblast-to-myelination stage by imaging mass spectrometry. The results demonstrated that short-chain sulfatides with 16 carbon non-hydroxylated fatty acids (C16) and 18 carbon non-hydroxylated fatty acids (C18) or 18 carbon hydroxylated fatty acids (C18-OH) existed in restricted regions of the early embryonic spinal cord, where pro-oligodendroblasts initially appear, and co-localized with Olig2-positive pro-oligodendroblasts. C18 and C18-OH sulfatides also existed in isolated pro-oligodendroblasts. C22-OH sulfatide became predominant later in oligodendrocyte development and the longer C24 sulfatide was predominant in the adult brain. Additionally, the presence of each sulfatide species in a different area of the adult brain was demonstrated by imaging mass spectrometry at an increased lateral resolution. These findings indicated that O4 recognized sulfatides with short-chain fatty acids in pro-oligodendroblasts. Moreover, the fatty acid chain of the sulfatide became longer as the oligodendrocyte matured. Therefore, individual sulfatide species may have unique roles in oligodendrocyte maturation and myelination. Read the Editorial Highlight for this article on page 356.

show abstract

mentioning

confidence: 99%

Sulfatide species with various fatty acid chains in oligodendrocytes at different developmental stages determined by imaging mass spectrometry

Hirahara

Wakabayashi

Mori

et al. 2016

Journal of Neurochemistry

Self Cite

View full text Add to dashboard Cite

show abstract

“…The IMS technique enables integration of numerous molecular weights and spatial distributions (Romero‐Perez, Takei, & Yao, ). MALDI, is a powerful high‐throughput method that has recently been developed, describes the direct measurement of sphingolipid from tissue extracts using 9AA as the matrix (Cheng, Sun, Yang, Gross, & Han, ; Eckhardt et al, ).…”

Section: Discussionmentioning

confidence: 99%

Change in phospholipid species of retinal layer in traumatic optic neuropathy model

Hirahara

Wakabayashi

Koike

et al. 2019

J of Neuroscience Research

View full text Add to dashboard Cite

Injured optic nerves induce death in almost all retinal ganglion cells (RGC) and cause a loss of axons. To date, we have studied injured RGC axon regeneration by using a traumatic optic nerve injury (TONI) rodent model, and we revealed that axonal regeneration is induced by the graft of an autologous peripheral nerve. The efficient approach to the regeneration of axons thus needs an environmental adjustment of RGC. However, the RGC environment induced by TONI remains unknown. Here, we analyzed female and male C57BL/6 mouse retinal tissue alterations in detail after TONI and focused on the major phospholipid species that are enriched in the whole retina. Reactive astrocyte accumulation, glia scar formation, and demyelination were observed in the injured optic nerve area, while RGC cell death, astrocyte accumulation, and Glial fibrillary acidic protein (GFAP) positive Müller cell increases were detected in the retinal layer. Furthermore, phosphatidylinositol (PI) 18:0/20:4 was localized to three nuclear layer structures: the ganglion cell layer (GCL), the inner nuclear layer (INL), and the outer nuclear layer (ONL) in control retina; however, the localization of 18:0/20:4 PI in TONI was disturbed. Meanwhile, phosphatidylserine (PS) 18:0/22:6showed that the expression was specifically in the inner plexiform layer (IPL) with similar signal intensity in both cases. Other PS species and phosphatidylethanolamine (PE) were differentially localized in the retinal layer; however, the expressions of PE including docosahexaenoic acid (DHA) were affected by TONI. These results suggest that not only GCL but also other retinal layers were influenced by TONI.

show abstract

“…The most common matrices used are 2,5-dihydroxy benzoic acid (DHB) and CHCA for positive mode, 9-aminoacridine (9AA) for negative mode, and 1,5-diaminonaphthalene (DAN) for both positive and negative mode. The effectiveness of these matrices have been evaluated recently for several molecule classes (Romero-Perez, Takei & Yao, 2014;Perry et al, 2020). However, a variety of new matrices have been evaluated for use with MALDI-FTMS to improve ionization efficiency, observed signal intensity, and reproducibility to fully take advantage of the high spatial and mass measurement resolution.…”

Section: Matrix Applicationmentioning

confidence: 99%

Advances in High‐resolution Maldi Mass Spectrometry for Neurobiology

DeLaney

Phetsanthad

2020

Mass Spectrometry Reviews

View full text Add to dashboard Cite

Research in the field of neurobiology and neurochemistry has seen a rapid expansion in the last several years due to advances in technologies and instrumentation, facilitating the detection of biomolecules critical to the complex signaling of neurons. Part of this growth has been due to the development and implementation of high-resolution Fourier transform (FT) mass spectrometry (MS), as is offered by FT ion cyclotron resonance (FTICR) and Orbitrap mass analyzers, which improves the accuracy of measurements and helps resolve the complex biological mixtures often analyzed in the nervous system. The coupling of matrixassisted laser desorption/ionization (MALDI) with high-resolution MS has drastically expanded the information that can be obtained with these complex samples. This review discusses notable technical developments in MALDI-FTICR and MALDI-Orbitrap platforms and their applications toward molecules in the nervous system, including sequence elucidation and profiling with de novo sequencing, analysis of post-translational modifications, in situ analysis, key advances in sample preparation and handling, quantitation, and imaging. Notable novel applications are also discussed to highlight key developments critical to advancing our understanding of neurobiology and providing insight into the exciting future of this field.

show abstract

Imaging Mass Spectrometric Analysis of Neurotransmitters

Cited by 7 publications

References 65 publications

Sulfatide species with various fatty acid chains in oligodendrocytes at different developmental stages determined by imaging mass spectrometry

Sulfatide species with various fatty acid chains in oligodendrocytes at different developmental stages determined by imaging mass spectrometry

Change in phospholipid species of retinal layer in traumatic optic neuropathy model

Advances in High‐resolution Maldi Mass Spectrometry for Neurobiology

Contact Info

Product

Resources

About