Imaging Laser-Induced Fluorescence Detection at the Taylor Cone of Electrospray Ionization Mass Spectrometry

Szarka, Máté; Szigeti, Márton

doi:10.1021/acs.analchem.9b01028

Cited by 20 publications

(19 citation statements)

References 38 publications

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“…12 Recently, imaging LIF was also used to study the Taylor cone at the electrospray interface of a mass spectrometer with a high limit of detection (40 attomoles), ensuring simultaneous optical and MS detection of fluorophore-labeled sugars. 13 A previous study from our group also reported fluorescence from gaseous ions of a fluorescent dye under ambient conditions electrosprayed from a methanol solution. 3 As the ions get desolvated in the electrospray plume, solvent relaxation in their ground and excited electronic states disappears.…”

Section: Introductionmentioning

confidence: 94%

Fluorescence-Based Detection of the Desolvation Process of Protein Ions Generated in an Aqueous Electrospray Plume

2021

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A new experimental setup to study laser-induced fluorescence (LIF) from analytes at different locations in an electrospray plume has been developed. The high fluorescence collection efficiency (~2%) of the setup, along with a sensitive CCD detector, enabled the study of low ion concentrations (down to ~fM) in the plume. The use of small electrospray tip inner diameters (<1 μm) facilitated the fast desolvation of gaseous protein ions in an aqueous electrospray plume. Fluorescence spectra were acquired from specific locations along the plume axis in different aqueous electrospray plumes with three different analytes: a rhodamine dye and two proteins (ubiquitin and apomyoglobin) labeled with rhodamine dyes. To confirm the presence of gaseous ions, pure gas-phase fluorescence spectra were acquired in the vacuum of a modified ion trap mass spectrometer. These spectra were used to fit and confirm the presence of gaseous species in the corresponding spectra obtained from the electrospray plume. This study shows that with small inner diameter spray capillaries, gaseous protein ions generated at atmospheric pressure in an electrospray plume can be detected with fluorescence-based techniques. Fluorescence measurements can be used to study their structure in the electrospray plume as they transition from solution to gas-phase and in the early stages after desolvating from charged droplets. Other techniques can also be applied/coupled to further study gaseous biomolecular structure at ambient conditions immediately after desolvation.

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Section: Introductionmentioning

confidence: 94%

Fluorescence-Based Detection of the Desolvation Process of Protein Ions Generated in an Aqueous Electrospray Plume

2021

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“…Both systems were capable to detect 13 glycans. A universal approach, independent of the ESI interface design, for simultaneous fluorescence‐based quantitative and MS‐based qualitative assessment of IgG released glycans was recently proposed [77]. With fluorophore labeled sugars, the authors used an imaging laser‐induced fluorescence (iLIF) system at the Taylor cone of the electrospray interface to collect fluorescent signal.…”

Section: Ce‐based Characterization Of Critical Quality Attributes (Cqas) Of Mab Productsmentioning

confidence: 99%

Applications of capillary electrophoresis for biopharmaceutical product characterization

2021

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Capillary electrophoresis (CE), after being introduced several decades ago, has carved out a niche for itself in the field of analytical characterization of biopharmaceutical products. It does not only offer fast separation, high resolution in miniaturized format, but equally importantly represents an orthogonal separation mechanism to high‐performance liquid chromatography. Therefore, it is not surprising that CE‐based methods can be found in all major pharmacopoeias and are recommended for the analysis of biopharmaceutical products during process development, characterization, quality control, and release testing. Different separation formats of CE, such as capillary gel electrophoresis, capillary isoelectric focusing, and capillary zone electrophoresis are widely used for size and charge heterogeneity characterization as well as purity and stability testing of therapeutic proteins. Hyphenation of CE with MS is emerging as a promising bioanalytical tool to assess the primary structure of therapeutic proteins along with any impurities. In this review, we confer the latest developments in capillary electrophoresis, used for the characterization of critical quality attributes of biopharmaceutical products covering the past 6 years (2015–2021). Monoclonal antibodies, due to their significant share in the market, have been given prioritized coverage.

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“…However, sample preparation can also be beneficial for MS detection to improve ionization efficiencies, reduce in-and post-source decay (e.g., sialic acids) and improve the glycan identification in MS/MS approaches, by creating more informative fragments [99,100]. Szarka et al demonstrated the possibility to perform simultaneous quantitative (optical) and qualitative (mass) analysis of APTS-labeled glycans using a novel imaging-LIF (iLIF) detector coupled to a CE-ESI-MS system [101]. By placing the iLIF detector at the Taylor cone of the electrospray interface it provides a quantitative fluorescence signal, prior to the MS-detection that is used for the structural analysis of the labeled glycans.…”

Section: Released Glycan Levelmentioning

confidence: 99%

Glycosylation of biosimilars: Recent advances in analytical characterization and clinical implications

Duivelshof

Jiskoot

Beck

et al. 2019

Analytica Chimica Acta

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License: Article 25fa pilot End User Agreement This publication is distributed under the terms of Article 25fa of the Dutch Copyright Act (Auteurswet) with explicit consent by the author. Dutch law entitles the maker of a short scientific work funded either wholly or partially by Dutch public funds to make that work publicly available for no consideration following a reasonable period of time after the work was first published, provided that clear reference is made to the source of the first publication of the work. This publication is distributed under The Association of Universities in the Netherlands (VSNU) 'Article 25fa implementation' pilot project. In this pilot research outputs of researchers employed by Dutch Universities that comply with the legal requirements of Article 25fa of the Dutch Copyright Act are distributed online and free of cost or other barriers in institutional repositories. Research outputs are distributed six months after their first online publication in the original published version and with proper attribution to the source of the original publication. You are permitted to download and use the publication for personal purposes. All rights remain with the author(s) and/or copyrights owner(s) of this work. Any use of the publication other than authorised under this licence or copyright law is prohibited. If you believe that digital publication of certain material infringes any of your rights or (privacy) interests, please let the Library know, stating your reasons.

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Imaging Laser-Induced Fluorescence Detection at the Taylor Cone of Electrospray Ionization Mass Spectrometry

Cited by 20 publications

References 38 publications

Fluorescence-Based Detection of the Desolvation Process of Protein Ions Generated in an Aqueous Electrospray Plume

Fluorescence-Based Detection of the Desolvation Process of Protein Ions Generated in an Aqueous Electrospray Plume

Applications of capillary electrophoresis for biopharmaceutical product characterization

Glycosylation of biosimilars: Recent advances in analytical characterization and clinical implications

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