Imaging gold nanoparticles in living cell environments using heterodyne digital holographic microscopy

Abstract

This paper describes an imaging microscopic technique based on heterodyne digital holography where subwavelengthsized gold colloids can be imaged in cell environment. Surface cellular receptors of 3T3 mouse fibroblasts are labeled with 40 nm gold nanoparticles, and the biological specimen is imaged in a total internal reflection configuration with holographic microscopy. Due to a higher scattering efficiency of the gold nanoparticles versus that of cellular structures, accurate localization of a gold marker is obtained within a 3D mapping of the entire sample's scattered field, with a lateral precision of 5 nm and 100 nm in the x,y and in the z directions respectively, demonstrating the ability of holographic microscopy to locate nanoparticles in living cells environments.

Fish embryo multimodal imaging by laser Doppler digital holography

“…All the driving signals are synchronized by a common 10 MHz clock. Figure 2 illustrates our reconstruction procedure [4,5] with non-Doppler shifted images. We have considered here four phase holograms: H = (I 0 − I 2 ) + j(I 1 − I 3 ) (see Fig.…”

Laser Doppler holographic microscopy in transmission: application to fish embryo imaging

“…The reconstruction procedure is similar to the one used in previous works [50,51]. To illustrate reconstruction, let us consider 4 phase detection of the ballistic peak signal (no Doppler shift).…”

Comparative review of interferometric detection of plasmonic nanoparticles

“…This latter study identified that this approach would trail single particle photothermal interferometry by an order of magnitude or greater in SNR and that the need for a widely distributed reference field might cause sample damage when imaging cells. This limitation was explored further by this group [48], where they imaged nanoparticles in the presence of cells using a similar scheme. The interface of the cell with the evanescent field showed the cell structure in contact with the prism.…”