Imaging domains in model membranes with atomic force microscopy

Rinia, Hilde A.; Kruijff, Ben de

doi:10.1016/s0014-5793(01)02704-1

Cited by 119 publications

(81 citation statements)

References 42 publications

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“…8A). These defects allowed an estimate of 5-6 nm for the thickness of the bilayer plus the water layer between the support and the contact leaflet, in agreement with previously reported data (27,38). In contrast, when the lipidpeptide assemblies were fused onto the mica, the formed bilayers displayed numerous holes of different sizes, shapes, and depths.…”

Section: Fig 3 Atr-ftir Spectra Of Ejh Peptides In Both Aqueous Solsupporting

confidence: 90%

Reconstitution of Holin Activity with a Synthetic Peptide Containing the 1–32 Sequence Region of EJh, the EJ-1 Phage Holin

Haro

Vélez

Goormaghtigh

et al. 2003

Journal of Biological Chemistry

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Pneumococcal EJ-1 phage holin (EJh) is a hydrophobic polypeptide of 85 amino acid residues displaying lethal inner membrane disruption activity. To get an insight into holin structure and function, several peptides representing the different topological regions predicted by sequence analysis have been synthesized. Peptides were structurally characterized in both aqueous buffer and membrane environments, and their potential to induce membrane perturbation was determined. Among them, only the N-terminal predicted transmembrane helix increased the membrane permeability. This segment, only when flanked by the positive charged residues on its N-terminal side, which are present in the sequence of the full-length protein, folds into a major ␣-helix structure with a transmembrane preferential orientation. Fluorescein quenching experiments of Nterminal-labeled peptide evidenced the formation of oligomers of variable size depending on the peptideto-lipid molar ratio. The self-assembling tendency correlated with the formation of transmembrane pores that permit the release of encapsulated dextrans of various sizes. When analyzed by atomic force microscopy, peptide-induced membrane lesions are visualized as transbilayer holes. These findings are the first evidence for a lytic domain in holins and for the nature of membrane lesions caused by them.

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Section: Fig 3 Atr-ftir Spectra Of Ejh Peptides In Both Aqueous Solsupporting

confidence: 90%

Reconstitution of Holin Activity with a Synthetic Peptide Containing the 1–32 Sequence Region of EJh, the EJ-1 Phage Holin

Haro

Vélez

Goormaghtigh

et al. 2003

Journal of Biological Chemistry

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“…The results presented here are in agreement with a nanoscale heterogeneity of the plant PM and the presence on the membrane of very small areas (less than 300 nm) of various levels of order. This fits with the lipid organization defined at the nanometer scale by atomic force microscopy using ternary lipid mixture vesicles (Rinia and de Kruijff, 2001;El Kirat et al, 2010;Giocondi et al, 2010). Furthermore, the sterol dependency of this or ganization, demonstrated in this work by the cyclodextrin-induced modification of RGR distribution (Fig.…”

Section: Spatial Distribution and Dynamic Of Pm Heterogeneitysupporting

confidence: 86%

Modification of Plasma Membrane Organization in Tobacco Cells Elicited by Cryptogein

et al. 2013

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Lipid mixtures within artificial membranes undergo a separation into liquid-disordered and liquid-ordered phases. However, the existence of this segregation into microscopic liquid-ordered phases has been difficult to prove in living cells, and the precise organization of the plasma membrane into such phases has not been elucidated in plant cells. We developed a multispectral confocal microscopy approach to generate ratiometric images of the plasma membrane surface of Bright Yellow 2 tobacco (Nicotiana tabacum) suspension cells labeled with an environment sensitive fluorescent probe. This allowed the in vivo characterization of the global level of order of this membrane, by which we could demonstrate that an increase in its proportion of ordered phases transiently occurred in the early steps of the signaling triggered by cryptogein and flagellin, two elicitors of plant defense reactions. The use of fluorescence recovery after photobleaching revealed an increase in plasma membrane fluidity induced by cryptogein, but not by flagellin. Moreover, we characterized the spatial distribution of liquid-ordered phases on the membrane of living plant cells and monitored their variations induced by cryptogein elicitation. We analyze these results in the context of plant defense signaling, discuss their meaning within the framework of the "membrane raft" hypothesis, and propose a new mechanism of signaling platform formation in response to elicitor treatment.

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“…Similar lipid domain patterns have been found in other lipid bilayer systems using various experimental techniques, including fluorescence spectroscopy [37], fluorescence microscopy [38], single-particle tracking techniques [39], scattering techniques [40], and atomic-force microscopy [41,42]. In particular, direct imaging techniques like atomic force microscopy applied to solid-supported bilayers and fluorescence microscopy applied to solid-supported lipid bilayers or giant unilamellar vesicles have revealed lipid domains in the range of tens of nanometers to microns, depending on the lipid membrane in question.…”

Section: Lateral Microstructure Of Lipid Bilayers and Its Influence Osupporting

confidence: 63%

Rational Design of a Liposomal Drug Delivery System Based on Biophysical Studies of Phospholipase A ₂ Activity on Model Lipid Membranes

Jørgensen

Davidsen

Andresen

et al. 2004

Lipases and Phospholipases in Drug Development

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Secretory phospholipase A 2 (sPLA 2 ) belongs to a family of small interfacially active enzymes that catalyze the hydrolysis of phospholipids, yielding lysolipids and fatty acids. sPLA 2 is found in snake and bee venom and also in inflammatory and cancerous tissue. It is mainly active on organized lipid substrates such as micelles and lipid membranes, and its hydrolytic activity is moreover strongly influenced by the physical properties of the supramolecular lipid substrate. Systematic model studies of the activity of sPLA 2 on lipid-membrane substrates of different compositions have provided insight into the biophysical mechanisms of sPLA 2 activation. In particular, combined theoretical and experimental model studies have revealed an intimate relationship between the activity of sPLA 2 and the formation of small-scale lipid domains in the lipid membrane substrate. This has been used to design and develop a principle for liposomal drug targeting, release, and absorption that is based on the presence of high levels of sPLA 2 activity in certain diseased target tissue.

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Imaging domains in model membranes with atomic force microscopy

Cited by 119 publications

References 42 publications

Reconstitution of Holin Activity with a Synthetic Peptide Containing the 1–32 Sequence Region of EJh, the EJ-1 Phage Holin

Reconstitution of Holin Activity with a Synthetic Peptide Containing the 1–32 Sequence Region of EJh, the EJ-1 Phage Holin

Modification of Plasma Membrane Organization in Tobacco Cells Elicited by Cryptogein

Rational Design of a Liposomal Drug Delivery System Based on Biophysical Studies of Phospholipase A ₂ Activity on Model Lipid Membranes

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Imaging domains in model membranes with atomic force microscopy

Cited by 119 publications

References 42 publications

Reconstitution of Holin Activity with a Synthetic Peptide Containing the 1–32 Sequence Region of EJh, the EJ-1 Phage Holin

Reconstitution of Holin Activity with a Synthetic Peptide Containing the 1–32 Sequence Region of EJh, the EJ-1 Phage Holin

Modification of Plasma Membrane Organization in Tobacco Cells Elicited by Cryptogein

Rational Design of a Liposomal Drug Delivery System Based on Biophysical Studies of Phospholipase A 2 Activity on Model Lipid Membranes

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Rational Design of a Liposomal Drug Delivery System Based on Biophysical Studies of Phospholipase A ₂ Activity on Model Lipid Membranes