Imaging Dedicated and Multifunctional Neural Circuits Generating Distinct Behaviors

Briggman, Kevin L.; Kristan, William B.

doi:10.1523/jneurosci.3265-06.2006

Cited by 147 publications

(153 citation statements)

References 33 publications

(50 reference statements)

Supporting

Mentioning

140

Contrasting

Unclassified

Order By: Relevance

“…The current results and other recent findings (Svoboda and Fetcho, 1996 2005) suggest that generation of several types of vertebrate hindlimb motor patterns may be mediated by a combination of shared and specialized circuitry, as has been seen in more numerous and detailed studies in invertebrates (Morton and Chiel, 1994;Marder and Calabrese, 1996;Kristan and Shaw, 1997;Edwards et al, 1999;Kupfermann and Weiss, 2001;Jing et al, 2004;Briggman et al, 2005;Marder et al, 2005;Briggman and Kristan, 2006). Moreover, the current results and a recent study in zebrafish (Ritter et al, 2001) both suggest that these shared and specialized subsets of spinal interneurons may to some extent be morphologically distinguishable.…”

Section: Morphological Comparisons To Dorsal Horn Cells In Other Studiessupporting

confidence: 79%

“…Cellular mechanisms of behavioral choice are best understood for several invertebrate systems, in which many CNS neurons contribute to multiple behaviors, whereas others are dedicated to just one (Morton and Chiel, 1994;Marder and Calabrese, 1996;Kristan and Shaw, 1997;Edwards et al, 1999;Kupfermann and Weiss, 2001;Jing et al, 2004;Briggman et al, 2005;Marder et al, 2005;Briggman and Kristan, 2006). In some cases, CNS neurons dedicated to one behavior inhibit CNS neurons dedicated to another behavior.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Spinal Interneurons That Are Selectively Activated during Fictive Flexion Reflex

Berkowitz¹

2007

J. Neurosci.

View full text Add to dashboard Cite

Behavioral choices in invertebrates are mediated by a combination of shared and specialized circuitry, including neurons that are inhibited during competing behaviors. Less is known, however, about the neural mechanisms of behavioral choice in vertebrates. The spinal cord can appropriately select among several types of limb movements, including limb withdrawal (flexion reflex), scratching, and locomotion, and thus is conducive to examination of vertebrate mechanisms of behavioral choice. Flexion reflex can interrupt and reset the rhythm of scratching and locomotion, suggesting that a combination of shared and specialized circuitry contributes to these behaviors, but little is known about the interneurons involved. Here, I used in vivo intracellular recording and dye injection to identify a group of spinal interneurons that are strongly activated during fictive flexion reflex but inhibited during fictive scratching and fictive swimming. These flexion-selective interneurons are typically rhythmically hyperpolarized during fictive scratching and fictive swimming. This hyperpolarization can be maximal during the ipsilateral hip flexor bursts of rhythmic limb motor patterns, although these cells are strongly activated during the ipsilateral hip flexor bursts of fictive flexion reflex. Thus, these interneurons are relatively specialized for fictive limb withdrawal, rather than contributing to the hip flexor phase of multiple types of limb movements. These flexion-selective cells are physiologically and morphologically distinguishable from a recently described group of spinal interneurons (transverse interneurons) that are strongly activated during both fictive flexion reflex and fictive scratching. Thus, spinal interneurons with distinct behavioral roles may to some extent be morphologically distinguishable.

show abstract

Section: Morphological Comparisons To Dorsal Horn Cells In Other Studiessupporting

confidence: 79%

Section: Introductionmentioning

confidence: 99%

Spinal Interneurons That Are Selectively Activated during Fictive Flexion Reflex

Berkowitz¹

2007

J. Neurosci.

View full text Add to dashboard Cite

show abstract

“…Existing studies generally fail to address the question of just how perfect they may be because they assume that a network supports specific physiological interactions (3)(4)(5)(6) or average the pattern of real connections, or they assume that connections are both exclusive and optimal. However rare, wiring errors violate these assumptions and can only be found by comparing multiple complete examples of the same anatomical wiring diagram.…”

mentioning

confidence: 99%

Synaptic circuits and their variations within different columns in the visual system of Drosophila

Takemura

Lǚ

et al. 2015

Proc. Natl. Acad. Sci. U.S.A.

265

379

View full text Add to dashboard Cite

We reconstructed the synaptic circuits of seven columns in the second neuropil or medulla behind the fly's compound eye. These neurons embody some of the most stereotyped circuits in one of the most miniaturized of animal brains. The reconstructions allow us, for the first time to our knowledge, to study variations between circuits in the medulla's neighboring columns. This variation in the number of synapses and the types of their synaptic partners has previously been little addressed because methods that visualize multiple circuits have not resolved detailed connections, and existing connectomic studies, which can see such connections, have not so far examined multiple reconstructions of the same circuit. Here, we address the omission by comparing the circuits common to all seven columns to assess variation in their connection strengths and the resultant rates of several different and distinct types of connection error. Error rates reveal that, overall, <1% of contacts are not part of a consensus circuit, and we classify those contacts that supplement (E+) or are missing from it (E−). Autapses, in which the same cell is both presynaptic and postsynaptic at the same synapse, are occasionally seen; two cells in particular, Dm9 and Mi1, form ≥20-fold more autapses than do other neurons. These results delimit the accuracy of developmental events that establish and normally maintain synaptic circuits with such precision, and thereby address the operation of such circuits. They also establish a precedent for error rates that will be required in the new science of connectomics.neural circuits | stereotypy | biological error rates | reconstruction error rates

show abstract

“…On the other hand, optical recording typically requires compound microscopes, with illumination systems and objectives better suited to delivering maximum light to the imaging device. Unfortunately, the short working distances, typically inverted images, and particularly the lack of stereopsis of compound microscopes makes working with multiple intracellular electrodes so difficult that few use them routinely in optical recording experiments (see however, (Briggman and Kristan, 2006;Taylor et al, 2003)). …”

Section: Introductionmentioning

confidence: 99%

A stereo-compound hybrid microscope for combined intracellular and optical recording of invertebrate neural network activity

Frost¹,

Wang²,

Brandon³

2007

Journal of Neuroscience Methods

View full text Add to dashboard Cite

Optical recording studies of invertebrate neural networks with voltage-sensitive dyes seldom employ conventional intracellular electrodes. This may in part be due to the traditional reliance on compound microscopes for such work. While such microscopes have high light-gathering power, they do not provide depth of field, making working with sharp electrodes difficult. Here we describe a hybrid microscope design, with switchable compound and stereo objectives, that eases the use of conventional intracellular electrodes in optical recording experiments. We use it, in combination with a voltage-sensitive dye and photodiode array, to identify neurons participating in the swim motor program of the marine mollusk Tritonia. This microscope design should be applicable to optical recording studies in many preparations.

show abstract

Imaging Dedicated and Multifunctional Neural Circuits Generating Distinct Behaviors

Cited by 147 publications

References 33 publications

Spinal Interneurons That Are Selectively Activated during Fictive Flexion Reflex

Spinal Interneurons That Are Selectively Activated during Fictive Flexion Reflex

Synaptic circuits and their variations within different columns in the visual system of Drosophila

A stereo-compound hybrid microscope for combined intracellular and optical recording of invertebrate neural network activity

Contact Info

Product

Resources

About