Imaging Characteristics of a Small Germanium Camera

Kaufman, Léon; Hosier, K.; Lorenz, V.; Shosa, D; Hoenninger, J; Cheng, A.; Okerlund, Michael D.; Hattner, Robert S.; Price, David C.; Williams, Stephen; Ewins, J.H.; Armantrout, G.A.; Camp, D.C.; Lee, K

doi:10.1097/00004424-197805000-00008

Cited by 19 publications

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“…Animals were scanned for 30-60 min on the High-Purity germanium gamma camera. The essential features of the camera's structure, parameters, and performance were already described [12,13]. The 131I and 99mTc images were separately obtained with a 353-366 and a 136-145 KeV window, respectively.…”

Section: Tumor Imagingmentioning

confidence: 99%

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Localization of human breast tumors grafted in nude mice with a monoclonal antibody directed against a defined cell surface antigen of human mammary epithelial cells

Ceriani

Sasaki

Orthendahl³

et al. 1988

Breast Cancer Res Tr

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A mouse monoclonal antibody (BLMRL-HMFG-Mc5) prepared against a defined cell surface antigen of human mammary epithelial cells, non-penetrating glycoprotein (NPGP), was used in imaging and distribution studies in athymic nude mice grafted with human breast tumors. For in vivo tissue distribution studies, 125I-labeled monoclonal antibody was injected into nude mice carrying simulated metastases of human tumors (breast and colon carcinomas). After 22-24 hr the amount of radioactivity per gram of tissue was 3-4 times higher in the breast tumor than in liver, brain, lung, muscle, or spleen. In contrast, colon carcinoma tissue, grafted and treated likewise, did not show higher accumulation of radioactivity relative to other tissues. At 4 days, the incorporation in breast tumors remained almost as high, while the circulating radioactive tracer and the incorporation in tissues other than breast had fallen significantly. In tumor imaging studies, breast tumor masses as small as 4 mm in diameter were clearly localized on a whole body scan using 131I-labeled BLMRL-HMFG-Mc5 antibodies with a High-Purity germanium gamma camera. Normalization of 131I-distribution to that of 99mTc-pertechnetate increased the specificity of this imaging methodology. The quantitative density of 131I-label was 2-3 fold higher over the breast tumor than over comparable areas of the mouse. No positive localization images were obtained for similar implants of colon and lung carcinomas or melanomas after injections of 131I-labeled BLMRL-HMFG-Mc5. Localization of human breast tumors in this model can be achieved with 131I-labeled anti-breast epithelial monoclonal antibodies.

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Section: Tumor Imagingmentioning

confidence: 99%

“…Similarly, using a High-Purity germanium camera [12] in the mouse system using Fab fragments of a polyclonal antibody against cell surface .components of mouse mammary epithelial cells, mouse mammary tumors were distinctively and specifically imaged [13,14].…”

Section: Introductionmentioning

confidence: 99%

Localization of human breast tumors grafted in nude mice with a monoclonal antibody directed against a defined cell surface antigen of human mammary epithelial cells

Ceriani

Sasaki

Orthendahl³

et al. 1988

Breast Cancer Res Tr

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Localization of mammary tumors in vivo with131i-labeled fab fragments of antibodies against mouse mammary epithelial (mme) antigens

Wilbanks

Peterson

Miller

et al. 1981

Cancer

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The Fab fragments of antibodies against cell-type-specific surface antigens of mouse mammary epithelial cells (MME-antigens) were used to localize mammary tumors successfully. The radioiodine-labeled anti-MME (Fab) was injected into mice carrying simulated mammary metastases, and after 24 hours the amount of label per gram of excised tissue was several times greater in the tumor than in liver, brain, lung, or muscle. Kidney showed considerable accumulation of label but this appeared to be nonspecific. Kinetic studies revealed a rapid elimination of labeled Pab in the urine with only 1% of the injected dose remaining in the entire blood pool after 24 hours. With a high-purity germanium camera, mammary tumors were clearly located by the '"'I-labeled anti-MME (Fab), and normaliza-tion to S!""Tc-pertechnetate distribution in the animal increased the specificity. The density of ':1'1-label was fourfold greater over the mammary tumor than over comparable areas of the mouse. No accumulation of ""1-anti-MME (Fab) was observed in nonmammary tumors nor in mammary tumors when labeled nonspecific Fab was used. An analogous system using an antihuman mammary epithelial antiserum is being developed for localization of breast metastases in humans. Cnncer 48:1768-1775, 1981. P P R o w i A I I T H E R A P Y for breast cancer depends A heavily upon reliable detection and tumor staging. The ability to detect hitherto unknown metastases and more accurately determine the extent of lymph node involvement can radically change the therapeutic approach ."' Also. the detection of distant metastases (e. g. , bone. liver) will modify treatment and prognosis even further. Noninvasive procedures currently used for detection of metastatic spread in breast cancer are similar to those used for other cancers. These include palpation. conventional radiography, CAT scanning, and also the use of radionuclides in sensitive but nonspecific techniques. such as bone scans. liver scans. and brain scans.:'!' With these techniques. cer

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Single Photon Imaging

Buvat

2013

Photon‐Based Medical Imagery

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Imaging Characteristics of a Small Germanium Camera

Cited by 19 publications

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Localization of human breast tumors grafted in nude mice with a monoclonal antibody directed against a defined cell surface antigen of human mammary epithelial cells

Localization of human breast tumors grafted in nude mice with a monoclonal antibody directed against a defined cell surface antigen of human mammary epithelial cells

Localization of mammary tumors in vivo with131i-labeled fab fragments of antibodies against mouse mammary epithelial (mme) antigens

Single Photon Imaging

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