Imaging and measuring the rituximab-induced changes of mechanical properties in B-lymphoma cells using atomic force microscopy

Li, Mi; Liu, Lianqing; Xi, Ning; Wang, Yuechao; Dong, Zaili; Tabata, Osamu; Xiao, Xiubin; Zhang, Weijing

doi:10.1016/j.bbrc.2010.12.043

Cited by 49 publications

(45 citation statements)

References 42 publications

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“…Atomic force microscopy (AFM) [11] provides nanoscale topographic resolution and can dynamically observe changes in mechanical properties of single cells, such as those following stimulation by drugs [12,13]. These aspects have made AFM become an important tool for the measurement of mechanical properties of biomaterials [14,15], and those of cells [16,17].…”

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confidence: 99%

Atomic force microscopy imaging and mechanical properties measurement of red blood cells and aggressive cancer cells

Liu

et al. 2012

Sci. China Life Sci.

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Mechanical properties play an important role in regulating cellular activities and are critical for unlocking the mysteries of life. Atomic force microscopy (AFM) enables researchers to measure mechanical properties of single living cells under physiological conditions. Here, AFM was used to investigate the topography and mechanical properties of red blood cells (RBCs) and three types of aggressive cancer cells (Burkitt's lymphoma Raji, cutaneous lymphoma Hut, and chronic myeloid leukemia K562). The surface topography of the RBCs and the three cancer cells was mapped with a conventional AFM probe, while mechanical properties were investigated with a micro-sphere glued onto a tip-less cantilever. The diameters of RBCs are significantly smaller than those of the cancer cells, and mechanical measurements indicated that Young's modulus of RBCs is smaller than those of the cancer cells. Aggressive cancer cells have a lower Young's modulus than that of indolent cancer cells, which may improve our understanding of metastasis. atomic force microscopy, red blood cell, cancer cell, mechanical properties, Young's modulus Citation:Li M, Liu L Q, Xi N, et al. Atomic force microscopy imaging and mechanical properties measurement of red blood cells and aggressive cancer cells. Sci China Life Sci, 2012, 55: 968 -973,

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Atomic force microscopy imaging and mechanical properties measurement of red blood cells and aggressive cancer cells

Liu

et al. 2012

Sci. China Life Sci.

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“…For each cell measured, 20 force curves were obtained using the same loading rate for each one. For computing the Young's modulus, the Hertz model was used (Touhami et al 2003;Li et al 2011):…”

Section: Mechanical Properties Measurementmentioning

confidence: 99%

Selective pattern of cancer cell accumulation and growth using UV modulating printing of hydrogels

Wei

et al. 2015

Biomed Microdevices

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Fabrication of extracellular microenvironment for cancer cell growth in vitro is an indispensable technique to precisely control the cell spatial arrangement and proliferation for cell-behavior research. Current micropatterning methods usually require relatively complicated operations, which makes it difficult to investigate the effects of different cell growth patterns. This manuscript proposes a DMD-based projection technique to quickly pattern a poly(ethylene) glycol diacrylate (PEGDA)-based hydrogel on a common glass substrate. Using this method, we can effectively control the growth patterns of cells. Compared with these traditional methods which employ digital dynamic mask, polymerization of PEGDA solution can be used to create arbitrary shaped microstructures with high efficiency, flexibility and repeatability. The duration of UV exposure is less than 10 s through controlling the projected illumination pattern. The ability of patterned PEGDA-coated film to hinder cell adhesion makes it possible to control area over which cells attach. In our experiments, we take advantage of the blank area to pattern cells, which allows cells to grow in various pre-designed shapes and sizes. And the patterning cells have a high viability after culturing for several days. Interestingly, we found that the restricted space could stiffen and strengthen the cells. These results indicate that cells and extracellular microenvironment can influence each other.

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“…Mammalian suspended cells are soft and have large size, causing the porous polymer membrane method or pure electrostatic adsorption unsuitable. In order to observe the changes of nanostructures on living lymphoma cells (a type of human suspended cell) during the rituximab's PCD mechanism, we have developed an immobilization method combining micro-fabricated pillar's mechanical trapping and poly-L-lysine electrostatic adsorption [22,23], as shown in Fig. 2.…”

Section: Pcd Mechanismmentioning

confidence: 99%

“…However, due to the limit of 200-nm resolution, traditional optical microscopy cannot reveal the dynamic developments of MACs on the cell Fig. 2 Changes of cellular nanostructures and mechanical properties during the PCD mechanism of rituximab revealed by AFM [22,23]. a SEM image of the micro-fabricated pillars.…”

Section: Cdc Mechanismmentioning

confidence: 99%

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Applications of Atomic Force Microscopy in Exploring Drug Actions in Lymphoma-Targeted Therapy at the Nanoscale

Liu

et al. 2015

BioNanoSci.

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Rituximab, a monoclonal antibody against the CD20 molecule expressed on B cells, has revolutionized the treatment of B cell lymphomas. The use of rituximab significantly improves the long-term survival rates of lymphoma patients. However, there are still many patients who develop resistance to rituximab. Hence, the urgent need is enhancing the potency of anti-CD20 antibodies beyond that achieved with rituximab to provide effective therapies for more patients. Traditional studies about the killing mechanisms of rituximab are commonly based on optical microscopy, which cannot reveal the detailed situations due to the limit of 200-nm resolution. Quantitatively investigating the actions of rituximab at the nanoscale is still scarce. The advent of atomic force microscopy (AFM) provides a powerful and versatile platform for in situ investigating the nanoscale behaviors of individual live cells. The applications of AFM in life sciences in the past decade have yielded a lot of novel insights into cell biology and related diseases. In this article, the typical applications (e.g., ultra-microstructures, mechanical properties, and molecular recognition) of AFM in investigating the three killing mechanisms of rituximab at the nanoscale are summarized; the challenges facing AFM single-cell assay and its future directions are also discussed.

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Imaging and measuring the rituximab-induced changes of mechanical properties in B-lymphoma cells using atomic force microscopy

Cited by 49 publications

References 42 publications

Atomic force microscopy imaging and mechanical properties measurement of red blood cells and aggressive cancer cells

Atomic force microscopy imaging and mechanical properties measurement of red blood cells and aggressive cancer cells

Selective pattern of cancer cell accumulation and growth using UV modulating printing of hydrogels

Applications of Atomic Force Microscopy in Exploring Drug Actions in Lymphoma-Targeted Therapy at the Nanoscale

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