Imaging Acute Renal Failure with Polyamine Dendrimer-Based MRI Contrast Agents

Dear, James W.; Kobayashi, Hisataka; Brechbiel, Martin W.; Star, Robert A.

doi:10.1159/000090608

Cited by 29 publications

(29 citation statements)

References 39 publications

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“…Although renal biopsy is rarely performed in human AKI, the presence of ATN is one of the most crucial characteristics for distinguishing AKI from oliguria caused by dehydration or prerenal azotemia. Recently developing imaging techniques such as magnetic resonance (MR) 22 might be applied in future study to evaluate the correlation of urinary L-FABP with morphological changes in the human kidney. We also demonstrate that urinary L-FABP can detect and predict functional decline in CP and IR AKI models.…”

Section: Discussionmentioning

confidence: 99%

Monitoring of Urinary L-Type Fatty Acid-Binding Protein Predicts Histological Severity of Acute Kidney Injury

Negishi

Noiri

Doi

et al. 2009

The American Journal of Pathology

106

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The present study aimed to evaluate whether levels of urinary L-type fatty acid-binding protein (L-FABP) could be used to monitor histological injury in acute kidney injury (AKI) induced by cis-platinum (CP) injection and ischemia reperfusion (IR). Different degrees of AKI severity were induced by several renal insults (CP dose and ischemia time) in human L-FABP transgenic mice. Renal histological injury scores increased with both CP dose and ischemic time. In CPinduced AKI, urinary L-FABP levels increased exponentially even in the lowest dose group as early as 2 hours, whereas blood urea nitrogen (BUN) levels increased at 48 hours. In IR-induced AKI, BUN levels increased only in the 30-minute ischemia group 24 hours after reperfusion; however, urinary L-FABP levels increased more than 100-fold, even in the 5-minute ischemia group after 1 hour. In both AKI models, urinary L-FABP levels showed a better correlation with final histological injury scores and glomerular filtration rates measured by fluorescein isothiocyanate-labeled inulin injection than with levels of Acute kidney injury (AKI) is still a critical problem because the mortality rate of severely ill patients complicated with AKI is much higher than non-AKI patients. 1There is no effective treatment for human AKI except for supportive renal replacement therapy such as dialysis. Novel renal biomarkers are indispensable to develop a new therapeutic strategy for AKI because it will allow us to detect AKI and start treatment early.2 In addition to early detection, renal biomarkers should be able to estimate renal injury accurately. Because acute tubular necrosis (ATN) is the common feature of most AKI, predicting histological injuries in renal tubular cells would be one of the required characteristics of renal biomarkers in AKI.We have recently demonstrated that L-type fatty acidbinding protein (L-FABP) is localized to proximal tubular cells in human kidney and projected to urinary space after ischemia reperfusion (IR) injury in living-related kidney transplantation.3 In human AKI after cardiac surgery, urinary L-FABP levels were increased at 4 hours after surgery in AKI patients at least, whereas serum creatinine started to increase 48 hours later.4 Mouse L-FABP in proximal tubules of C57BL/6 wild-type mice was virtually defective because of the silencing sequence localizing in the upstream of the promoter region. Therefore, we used human L-FABP transgenic mice, which are humanized by expressing higher protein levels of human L-FABP in

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Section: Discussionmentioning

confidence: 99%

Monitoring of Urinary L-Type Fatty Acid-Binding Protein Predicts Histological Severity of Acute Kidney Injury

Negishi

Noiri

Doi

et al. 2009

The American Journal of Pathology

106

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“…Dendrimers allow the precise control of size, shape and placement of functional groups that is advantageous for many life science applications. Their systematically variable structural architecture and large internal free volume make these dendrimers an unique class of molecule for various biomedical applications including drug (Na et al, 2006, Yiyun et al, 2005, DNA (GuillotNieckowski et al, 2007, Kukowska Latallo et al, 1996, and siRNA (Zhou et al, 2006) delivery, and as MRI probes (Dear et al, 2006, Swanson et al 2008 etc.…”

Section: Introductionmentioning

confidence: 99%

Mechanistic studies of in vitro cytotoxicity of poly(amidoamine) dendrimers in mammalian cells

Mukherjee¹,

Lyng²,

Garcia³

et al. 2010

Toxicology and Applied Pharmacology

154

141

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“…The use of a PAMAM dendrimer based MRI contrast agent which can detect renal injury prior to the elevation of creatinine levels has also been reported (Dear et al, 2006). PAMAM dendrimers of different generations have also been employed for enhancing the solubility of poorly watersoluble drugs, e.g., ketoprofen, whereby the drug solubility increases with the dendrimer concentration, pH and generation (Yiyun et al, 2005).…”

Section: Introductionmentioning

confidence: 99%

In vitro mammalian cytotoxicological study of PAMAM dendrimers – Towards quantitative structure activity relationships

Mukherjee¹,

Davoren²,

Byrne³

2010

Toxicology in Vitro

137

114

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Imaging Acute Renal Failure with Polyamine Dendrimer-Based MRI Contrast Agents

Cited by 29 publications

References 39 publications

Monitoring of Urinary L-Type Fatty Acid-Binding Protein Predicts Histological Severity of Acute Kidney Injury

Monitoring of Urinary L-Type Fatty Acid-Binding Protein Predicts Histological Severity of Acute Kidney Injury

Mechanistic studies of in vitro cytotoxicity of poly(amidoamine) dendrimers in mammalian cells

In vitro mammalian cytotoxicological study of PAMAM dendrimers – Towards quantitative structure activity relationships

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