Images of loop-mediated isothermal amplification (LAMP) for SARS-CoV-2 testing and optimized 'Cap-iLAMP'

Riesenberg, Stephan; Bokelmann, Lukas; Maricic, Tomislav; Pääbo, Svante; Meyer, Matthias; Nickel, Olaf; Borte, Stephan

doi:10.5061/dryad.2rbnzs7mk

2020

DOI: 10.5061/dryad.2rbnzs7mk

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Images of loop-mediated isothermal amplification (LAMP) for SARS-CoV-2 testing and optimized 'Cap-iLAMP'

Stephan Riesenberg,

Lukas Bokelmann,

Tomislav Maricic

et al.

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Rapid, reliable, and cheap point-of-care bulk testing for SARS-CoV-2 by combining hybridization capture with improved colorimetric LAMP (Cap-iLAMP)

Bokelmann

Nickel

Maričić

et al. 2020

Preprint

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Efforts to contain the spread of SARS-CoV-2 have spurred the need for reliable, rapid, and cost-effective diagnostic methods which can easily be applied to large numbers of people. However, current standard protocols for the detection of viral nucleic acids while sensitive, require a high level of automation, sophisticated laboratory equipment and trained personnel to achieve throughputs that allow whole communities to be tested on a regular basis. Here we present Cap-iLAMP (capture and improved loop-mediated isothermal amplification). This method combines a hybridization capture-based RNA extraction of non-invasive gargle lavage samples to concentrate samples and remove inhibitors with an improved colorimetric RT-LAMP assay and smartphone-based color scoring. Cap-iLAMP is compatible with point-of-care testing and enables the detection of SARS-CoV-2 positive samples in less than one hour. In contrast to direct addition of the sample to improved LAMP (iLAMP), Cap-iLAMP does not result in false positives and single infected samples can be detected in a pool among 25 uninfected samples, thus reducing the technical cost per test to ~1 Euro per individual.

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Rapid, reliable, and cheap point-of-care bulk testing for SARS-CoV-2 by combining hybridization capture with improved colorimetric LAMP (Cap-iLAMP)

Bokelmann

Nickel

Maričić

et al. 2020

Preprint

Self Cite

View full text Add to dashboard Cite

show abstract

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Images of loop-mediated isothermal amplification (LAMP) for SARS-CoV-2 testing and optimized 'Cap-iLAMP'

Cited by 1 publication

References 1 publication

Rapid, reliable, and cheap point-of-care bulk testing for SARS-CoV-2 by combining hybridization capture with improved colorimetric LAMP (Cap-iLAMP)

Rapid, reliable, and cheap point-of-care bulk testing for SARS-CoV-2 by combining hybridization capture with improved colorimetric LAMP (Cap-iLAMP)

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