Abstract-Several flow measurement methods have been developed to get more in depth information of two-phase flow. Digital image processing technique is one of the methods which provide many advantages. It is classified as non-intrusive methods and able to process a large number of images. Furthermore, this technique is also supported by the flexible algorithm and advanced technology in high speed camera. In slug flow, the presence of small bubble become one of the biggest challenges since it can distract slug interface. This work explains image processing procedures to get clear interface between the gas and liquid phase on horizontal slug flow. Then the image processing technique was applied to a set of slug flow data. The experiment was conducted in a horizontal pipeline with 50 mm inner diameter using the combination of superficial liquid velocity 0.2-0.77 m/s and superficial gas velocity 0.7 -1.88 m/s. The flow was recorded using high speed video camera and extracted into images sequence. Next, applied algorithm is well explained and the example of image processing result is presented. When the interface is clear, some slug flow parameter can be calculated like its velocity, length, and frequency. This method is able to reveal the effect of liquid superficial velocity and gas superficial velocity on slug flow parameter. Moreover, present data is also compared with previous research and shows good agreement.