Image panorama without loss of focusing for microscopic systems

A Method for Medical Microscopic Images’ Sharpness Evaluation Based on NSST and Variance by Combining Time and Frequency Domains

“…To obtain sharp images by controlling a motor to adjust the lens or the stage in a computer-controlled microscope imaging process, autofocusing adopts two main methods [ 2 ]: active focusing, based on the principle of distance measurement, and passive focusing, based on image processing. Because microscopic images are often taken with a high-magnification and small-aperture objective lens, the depth of field stays at the micron level, and thus, the mechanical system is very demanding.…”