Image-based vessel-by-vessel analysis for red blood cell and plasma dynamics with automatic segmentation

Kawaguchi, Hiroshi; Masamoto, Kazuto; Ito, Hiroshi; Kanno, Iwao

doi:10.1016/j.mvr.2012.05.001

Cited by 10 publications

(15 citation statements)

References 31 publications

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“…In this method, the plasma flow results in lectin binding to endothelial cells, even without moving red blood cells. However, Kawaguchi et al ( 2012 ) observed similar spatiotemporal behaviors for the plasma and red blood cells, being labeled by water-soluble Qdot and fluorescein isothiocyanate (FITC), respectively. Poole et al ( 2013 ) found that the majority of capillaries support blood flow in resting muscle, although plasma and red blood cells travel at different speeds through capillaries.…”

Section: Introductionmentioning

confidence: 99%

Regulation of the microvascular circulation in the leg muscles, pancreas and small intestine in rats

2015

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To study the microvascular circulation, we examined the proportion of open and functioning capillaries in the leg muscles, pancreas and small intestine of anesthetized rats. Fluorescein isothiocyanate (FITC)-labeled Lycopersicon esculentum lectin was injected into the heart and allowed to circulate for 3 min to label open and functioning capillaries. Specimens were removed, frozen, sectioned and double-immunostained. Using one section, open and functioning capillaries were detected by immunostaining for this lectin bound to endothelial cells, while all capillaries were visualized by immunostaining for platelet endothelial cell adhesion molecule-1 (PECAM-1 or CD31). These capillaries were semi-automatically detected and counted by fluorescence microscopy. The percentages of open and functioning capillaries were as follows: the soleus muscle, 93.0 ± 5.5%; superficial zone of the gastrocnemius muscle, 90.8 ± 6.2%; deep zone of the gastrocnemius muscle, 95.6 ± 4.0%; the plantaris muscle, 94.1 ± 2.7%; the pancreas, 86.3 ± 11.7%; and the small intestine, 91.1 ± 4.9% (n = 8, each). There was no significant difference among these data by the Kruskal–Wallis test. This study clearly demonstrated that the proportions of open and functioning capillaries are high and similar among the leg muscles, pancreas and small intestine in spite of their structural and functional differences. This finding agrees with previous studies and supports the notion that the microvascular circulation is mainly controlled by changing of the blood flow in each capillary rather than changing the proportion of open and functioning capillaries.

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Section: Introductionmentioning

confidence: 99%

Regulation of the microvascular circulation in the leg muscles, pancreas and small intestine in rats

2015

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“…To estimate the measurement errors in the present method, the velocities measured were compared with velocity measurements of the same vessels using a previously described cross-correlation method [13]. The results showed good linear correlation for ranges of the measured velocities in both rats and mice (R = 0.95 and 0.99, p < 0.05, respectively; Figure S3).…”

Section: Measurement Errormentioning

confidence: 95%

Dynamic Flow Velocity Mapping from Fluorescent Dye Transit Times in the Brain Surface Microcirculation of Anesthetized Rats and Mice

Hoshikawa

Kawaguchi

Takuwa³

et al. 2016

Microcirculation

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“…Theoretically, blood cells flow faster than the plasma in the small sizes of the vessels, which cause a decrease in peripheral hematocrit in these vessel regions. However, no consistent experimental data have been produced (Kawaguchi et al, 2012;Rovainen et al, 1993). This indicates spatial heterogeneity of plasma and blood cell flow in the parenchymal microcirculation.…”

Section: Plasma Vs Rbc Speedmentioning

confidence: 99%

Bridging macroscopic and microscopic methods for the measurements of cerebral blood flow

Kanno¹,

Masamoto

2016

Progress in Brain Research

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Image-based vessel-by-vessel analysis for red blood cell and plasma dynamics with automatic segmentation

Cited by 10 publications

References 31 publications

Regulation of the microvascular circulation in the leg muscles, pancreas and small intestine in rats

Regulation of the microvascular circulation in the leg muscles, pancreas and small intestine in rats

Dynamic Flow Velocity Mapping from Fluorescent Dye Transit Times in the Brain Surface Microcirculation of Anesthetized Rats and Mice

Bridging macroscopic and microscopic methods for the measurements of cerebral blood flow

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