Image Analysis using AI of Squamous Cell Carcinoma and Leukoplakia by Optical Instrument

Morikawa, Takamichi; Kosugi, Ayaka; Kozakai, Ayako; Marumo, Tomoko; Bessho, Hiroki; Shibahara, Takahiko

doi:10.5984/jjpnsoclaserdent.29.131

Cited by 5 publications

(22 citation statements)

References 27 publications

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“…On the other hand, concerning objective evaluation, LV was significantly lower in HRL than in Others according to multivariate analysis. In this study, although the protocol was kept constant, some degree of influence from environmental factors cannot be ruled out (14). However, using LRatio, the influence of environmental factors was reduced; thus, HRL was significantly lower than Others by multivariate analysis on LRatio.…”

Section: Discussionmentioning

confidence: 86%

“…Screening should be minimally invasive, repeatable and inexpensive. Cytology (9), vital staining (10,11), and FV (12)(13)(14)(15) are simple and effective as methods of screening for HRL. Oral brush cytology (OBC) is a well-tolerated, mildly invasive, safe approach for harvesting cells from the oral mucosa (9).…”

Section: Discussionmentioning

confidence: 99%

“…FV images were taken in a darkened room as much as possible, with a distance between the lesion and OI of about 10 cm. The irradiating light was set perpendicular to the lesion and the irradiation range was set to about 10 cm (14).…”

Section: Methodsmentioning

confidence: 99%

“…FV images were analyzed using Image J software version 1.5 (National Institutes of Health, Bethesda, Maryland, USA). A region of interest (ROI) for the lesion and control area was established in FV images (14,15).…”

Section: Methodsmentioning

confidence: 99%

“…In addition, color mapping was performed for the lesion ROI as a surface plot ( Figure 1D). The surface plot was used as a reference for subject evaluation (14,15).…”

Section: Methodsmentioning

confidence: 99%

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The Utility of Optical Instrument “ORALOOK®” in the Early Detection of High-risk Oral Mucosal Lesions

2019

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Background/Aim: Oral cancer screening is important for early detection and early treatment, which help improve survival rates. Biopsy is invasive and painful, while fluorescence visualization is non-invasive, convenient, and realtime, and examinations can be repeated using optical instruments. The purpose of this study was to clarify the usefulness of an optical instrument in oral screening. Patients and Methods: A total of 201 patients, who were examined using an optical instrument in our Department between 2017 and 2018, were enrolled in this study. Fluorescence visualization images were analyzed using subjective and objective evaluations. Results: Subjective evaluations for detecting oral cancer and oral epithelial dysplasia offered 83.3% sensitivity and 75.7% specificity. Regarding the objective evaluations for detecting oral cancer and oral epithelial dysplasia, sensitivity and specificity were 47.4% and 72.4% for luminance value, 94.7% and 79.6% for luminance ratio, and 100.0% and 68.0% coefficient of variation. Conclusion: Fluorescence visualization using optical instruments is useful for oral cancer screening.

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Section: Discussionmentioning

confidence: 86%

Section: Discussionmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

“…In addition, color mapping was performed for the lesion ROI as a surface plot ( Figure 1D). The surface plot was used as a reference for subject evaluation (14,15).…”

Section: Methodsmentioning

confidence: 99%

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The Utility of Optical Instrument “ORALOOK®” in the Early Detection of High-risk Oral Mucosal Lesions

2019

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Method for diagnosing neoplastic lesions by quantitative fluorescence value

Kosugi

Kasahara

Yang

et al. 2019

Sci Rep

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Fluorescence visualization devices (FVs) are useful for detecting malignant lesions because of their simple and noninvasive application. However, their quantitative application has been challenging. This study aimed to quantitatively and statistically evaluate the change in fluorescence intensity (FI) during the progression from normal epithelium to squamous cell carcinoma using a reproducible animal tongue carcinogenesis model. To establish this model, rats were treated with 50 ppm 4-Nitroquinoline 1-oxide (4NQO) in their drinking water for 10, 15, and 20 weeks. After 4NQO administration, each rat tongue was evaluated by gross observation, histology, and FI measurements. Fluorescence images were captured by FV, and ImageJ was used to measure FI, which was analyzed quantitatively and statistically. The establishment of a reproducible tumor progression model was confirmed, showing precancerous lesions (low-grade dysplasia [LGD]), early cancers (high-grade dysplasia/carcinoma in situ [HGD/CIS]), and advanced cancers (Cancer). This carcinogenesis model was quantitatively evaluated by FI. The FI of LGD stage was 54.6, which was highest intensity of all groups. Subsequently, the HGD/CIS and Cancer stages showed decreased FI (HGD/CIS: 46.1, Cancer: 49.1) and manifested as dark spots. This result indicates that FI had more variation and a wider range with increasing tumor progression. We demonstrated that FI migration and an uneven distribution are consistent with tumor progression. Since each step of tumor progression occurs reproducibly in this animal model, statistical evaluation was possible. In addition, tumor progression can be monitored by this new FI analysis method in humans.

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Early Detection of Leukoplakic Oral Squamous Cell Carcinoma Using 4NQO-induced Rat Tongue Cancer Model: Study Utilizing Fluorescence Intensity and Histopathological Evaluation

Masuda

Yamamoto

Shibahara

2022

Bull. Tokyo Dent. Coll.

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Early identification of leukoplakic oral squamous cell carcinoma (OSCC) is difficult. The purpose of this study was to determine whether it was possible to detect change from normal epithelium to leukoplakic OSCC using a fluorescence visualization (FV) device in a 4-nitroquinoline 1-oxide (4NQO) -induced rat tongue cancer model. If successful, this would facilitate early detection of OSCC. The rats (3 groups of 5) were administered 50 ppm 4NQO in their drinking water over a period of 10, 15, or 20 weeks. Five nontreated rats were used as a control group. Images of their tongues obtained by FV were analyzed for change in fluorescence intensity (FI) using image analysis software. Immunoreaction for anti-CK13, anti-CK17, and anti-E-cadherin antibodies was also histopathologically evaluated. Receiver operating characteristic (ROC) analysis was used to calculate the cut-off values, sensitivity, specificity, and area under the curve. The most marked change in FI was found between the control and 10-week groups, with an increase observed in its average value and range in the latter. These findings differed from those characteristic of leukoplakia. No significant difference was observed in the positive cell rate for immunoreaction for anti-CK13 or anti-CK17 antibodies between the control and 10-week groups. A significant decrease was observed in the positive pixel ratio of immunoreaction for anti-E-cadherin antibody in the 10-week group in comparison with in the control group (p <0.05). These results showed that disruption of intercellular adhesion could be observed at 10 weeks. In the ROC analysis, the FI cut-off value in the 10-week and control groups was 51.9, sensitivity 95.5%, and specificity 96.9%. This indicated that normal epithelium could be accurately distinguished from low-grade dysplasia with high probability. These results demonstrate that analysis of change in FI as measured by FV could facilitate early detection of leukoplakic OSCC.

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Image Analysis using AI of Squamous Cell Carcinoma and Leukoplakia by Optical Instrument

Cited by 5 publications

References 27 publications

The Utility of Optical Instrument “ORALOOK®” in the Early Detection of High-risk Oral Mucosal Lesions

The Utility of Optical Instrument “ORALOOK®” in the Early Detection of High-risk Oral Mucosal Lesions

Method for diagnosing neoplastic lesions by quantitative fluorescence value

Early Detection of Leukoplakic Oral Squamous Cell Carcinoma Using 4NQO-induced Rat Tongue Cancer Model: Study Utilizing Fluorescence Intensity and Histopathological Evaluation

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