Image Analysis of 3D Conjunctival Melanoma Cell Cultures Following Electrochemotherapy

Fiorentzis, Miltiadis; Katopodis, Periklis; Kalirai, Helen; Seitz, Berthold; Viestenz, Arne; Coupland, Sarah E.

doi:10.3390/biomedicines8060158

Cited by 5 publications

(4 citation statements)

References 43 publications

(72 reference statements)

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“…Peripherally located cells detach from the main spheroid body. ECT treatment changes the spheroid shape, alters the inner area core with necrotic cells, and the outer area consists of proliferative cells [ 116 , 117 ]. Bleomycin combined with electroporation reduced the viability of conjunctival cell lines (CRMM1, CRMM2).…”

Section: Electrically Enhanced Drug Deliverymentioning

confidence: 99%

New Perspectives for Eye-Sparing Treatment Strategies in Primary Uveal Melanoma

Bilmin

Synoradzki

Czarnecka

et al. 2021

Cancers

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Uveal melanoma is the most common intraocular malignancy and arises from melanocytes in the choroid, ciliary body, or iris. The current eye-sparing treatment options include surgical treatment, plaque brachytherapy, proton beam radiotherapy, stereotactic photon radiotherapy, or photodynamic therapy. However, the efficacy of these methods is still unsatisfactory. This article reviews several possible new treatment options and their potential advantages in treating localized uveal melanoma. These methods may be based on the physical destruction of the cancerous cells by applying ultrasounds. Two examples of such an approach are High-Intensity Focused Ultrasound (HIFU)—a promising technology of thermal destruction of solid tumors located deep under the skin and sonodynamic therapy (SDT) that induces reactive oxygen species. Another approach may be based on improving the penetration of anti-cancer agents into UM cells. The most promising technologies from this group are based on enhancing drug delivery by applying electric current. One such approach is called transcorneal iontophoresis and has already been shown to increase the local concentration of several different therapeutics. Another technique, electrically enhanced chemotherapy, may promote drug delivery from the intercellular space to cells. Finally, new advanced nanoparticles are developed to combine diagnostic imaging and therapy (i.e., theranostics). However, these methods are mostly at an early stage of development. More advanced and targeted preclinical studies and clinical trials would be needed to introduce some of these techniques to routine clinical practice.

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Section: Electrically Enhanced Drug Deliverymentioning

confidence: 99%

New Perspectives for Eye-Sparing Treatment Strategies in Primary Uveal Melanoma

Bilmin

Synoradzki

Czarnecka

et al. 2021

Cancers

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show abstract

“…Peripherally located cells detach from the main spheroid body. ECT treatment changes the spheroid shape, alters the inner area core with necrotic cells, and the outer area consists of proliferative cells [115,116]. Bleomycin combined with electroporation reduced the viability of conjunctival cell lines (CRMM1, CRMM2).…”

Section: Iontophoresismentioning

confidence: 99%

New Perspectives for Eye-Sparing Treatment Strategies in Primary Uveal Melanoma

Bilmin¹,

Synoradzki²,

Czarnecka³

et al. 2021

Preprint

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Uveal melanoma is the most common intraocular malignancy and arises from melanocytes in the choroid, ciliary body, or iris. Radiotherapy is the mainstay of therapy for most patients with localized uveal melanoma. Another RT technique used in the treatment of uveal melanomas is charged-particle radiotherapy. Photodynamic therapy is based on the selective destruction of cancer cells or pathological vessels. High-Intensity Focused Ultrasound (HIFU) is a promising technology of thermal destruction of solid tumors located deep under the skin. The principle of operation is based on the heating of a tumor. Sonodynamic therapy (SDT) induces the reactive oxygen species and kills cancer cells. Electroporation applied in vivo delivers drugs or genetic material from the intercellular space to cells. Iontophoresis is a technique in which using electric current increases the biodistribution of drugs in the eyeball. Transcorneal iontophoresis has been shown to increase the local concentration of antibacterial and antifungal drugs, steroids, DNA, and RNA molecules. Theranosticsincorporates diagnostic imaging and therapy. Although no theranostic markers have been developed specifically for uveal melanoma, some NPs have already found use in ophthalmology. UM presents an unmet clinical need. Novel eye-preserving therapeutic approaches for the localized disease are needed.

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“…The authors used the human conjunctival melanoma cell lines CRMM1 and CRMM2 to form spheroids, in which they analyzed the cytotoxic effect of electrochemotherapy and other chemical treatments, such as 5-fluorouracil, mitomycin C, or bleomycin. Afterward, they published an automated image analysis method that can be used for the assessment of tumor growth and changes in the size of 3D tumor organoids following anticancer treatments [ 166 ].…”

Section: Three-dimensional In Vitro Models Of Anterior Eye Tissuesmentioning

confidence: 99%

Three-Dimensional Human Cell Culture Models to Study the Pathophysiology of the Anterior Eye

García-Posadas

Diebold

2020

Pharmaceutics

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In recent decades, the establishment of complex three-dimensional (3D) models of tissues has allowed researchers to perform high-quality studies and to not only advance knowledge of the physiology of these tissues but also mimic pathological conditions to test novel therapeutic strategies. The main advantage of 3D models is that they recapitulate the spatial architecture of tissues and thereby provide more physiologically relevant information. The eye is an extremely complex organ that comprises a large variety of highly heterogeneous tissues that are divided into two asymmetrical portions: the anterior and posterior segments. The anterior segment consists of the cornea, conjunctiva, iris, ciliary body, sclera, aqueous humor, and the lens. Different diseases in these tissues can have devastating effects. To study these pathologies and develop new treatments, the use of cell culture models is instrumental, and the better the model, the more relevant the results. Thus, the development of sophisticated 3D models of ocular tissues is a significant challenge with enormous potential. In this review, we present a comprehensive overview of the latest advances in the development of 3D in vitro models of the anterior segment of the eye, with a special focus on those that use human primary cells.

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Image Analysis of 3D Conjunctival Melanoma Cell Cultures Following Electrochemotherapy

Cited by 5 publications

References 43 publications

New Perspectives for Eye-Sparing Treatment Strategies in Primary Uveal Melanoma

New Perspectives for Eye-Sparing Treatment Strategies in Primary Uveal Melanoma

New Perspectives for Eye-Sparing Treatment Strategies in Primary Uveal Melanoma

Three-Dimensional Human Cell Culture Models to Study the Pathophysiology of the Anterior Eye

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