Image Analysis in Surgical Pathology

Lloyd, Mark C.; Monaco, James

doi:10.1016/j.path.2016.02.001

Cited by 9 publications

(8 citation statements)

References 33 publications

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“…However, the applied digital techniques in this study enable objective and reproducible analyses without the need for extensive pathological expertise. With the integration of digital pathology into clinical practice, comparison of future studies with our results might be easier 43,44 . Although widely used to quantify MVD, CD34 is known to also identify lymph vessels and stem cell populations, which theoretically could have led to an overestimation of our results.…”

Section: Discussionmentioning

confidence: 79%

Tamoxifen and pregnancy: an absolute contraindication?

Schuurman

Witteveen

Wall

et al. 2019

Breast Cancer Res Treat

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BACKGROUND: Identification of endometrial carcinoma (EC) patients at high risk of recurrence is lacking. In this study, the prognostic role of hypoxia and angiogenesis was investigated in EC patients. METHODS:Tumor slides from EC patients were stained by immunofluorescence for CAIX as hypoxic marker and CD34 for assessment of microvessel density (MVD). CAIXexpression was determined in epithelial tumor cells, with a cut-off of 1%. MVD was assessed according to the Weidner method. Correlations with disease-specific survival (DSS), diseasefree survival (DFS) and distant disease-free survival (DDFS) were calculated using Kaplan-Meier curves and Cox regression analysis.RESULTS: Sixty-three (16.4%) of 385 ECs showed positive CAIX-expression with high vascular density. These ECs had a reduced DSS compared to tumors with either hypoxia or high vascular density (log-rank p=0.002). Multivariable analysis showed that hypoxic tumors with high vascular density had a reduced DSS (hazard ratio [HR] 3.71, p=0.002), DDFS (HR 2.68, p=0.009) and a trend for reduced DFS (HR 1.87, p=0.054). CONCLUSIONS:This study has shown that adverse outcome in hypoxic ECs is seen in the presence of high vascular density, suggesting an important role of angiogenesis in the metastatic process of hypoxic EC. Differential adjuvant treatment might be indicated for these patients.

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Section: Discussionmentioning

confidence: 79%

Tamoxifen and pregnancy: an absolute contraindication?

Schuurman

Witteveen

Wall

et al. 2019

Breast Cancer Res Treat

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show abstract

“…29,30 However, the applied digital With the integration of digital pathology into clinical practice, comparison of future studies with our results might be easier. 43,44 Although widely used to quantify MVD, CD34 is known to also identify lymph vessels and stem cell populations, which theoretically could have led to an overestimation of our results. On the other hand, other antibodies, for example, CD31, also carry the risk of aspecific staining.…”

Section: Discussionmentioning

confidence: 93%

Poor outcome in hypoxic endometrial carcinoma is related to vascular density

Reijnen¹,

Weelden²,

Arts

et al. 2019

Br J Cancer

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BACKGROUND: Identification of endometrial carcinoma (EC) patients at high risk of recurrence is lacking. In this study, the prognostic role of hypoxia and angiogenesis was investigated in EC patients. METHODS: Tumour slides from EC patients were stained by immunofluorescence for carbonic anhydrase IX (CAIX) as hypoxic marker and CD34 for assessment of microvessel density (MVD). CAIX expression was determined in epithelial tumour cells, with a cutoff of 1%. MVD was assessed according to the Weidner method. Correlations with disease-specific survival (DSS), disease-free survival (DFS) and distant disease-free survival (DDFS) were calculated using Kaplan-Meier curves and Cox regression analysis. RESULTS: Sixty-three (16.4%) of 385 ECs showed positive CAIX expression with high vascular density. These ECs had a reduced DSS compared to tumours with either hypoxia or high vascular density (log-rank p = 0.002). Multivariable analysis showed that hypoxic tumours with high vascular density had a reduced DSS (hazard ratio [HR] 3.71, p = 0.002), DDFS (HR 2.68, p = 0.009) and a trend for reduced DFS (HR 1.87, p = 0.054). CONCLUSIONS: This study has shown that adverse outcome in hypoxic ECs is seen in the presence of high vascular density, suggesting an important role of angiogenesis in the metastatic process of hypoxic EC. Differential adjuvant treatment might be indicated for these patients.

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“…Digital Pathology is a growing field and has been used in a few specific areas, such as in cancer diagnosis and biomarker analysis for decision making in cancer treatment (8, 9). The process starts with digitization of patient pathology samples, followed by pathological interpretation on a computer screen, or image analysis using algorithms to extract information of interest (10, 11).…”

Section: Introductionmentioning

confidence: 99%

Quantitative Characterization of CD8+ T Cell Clustering and Spatial Heterogeneity in Solid Tumors

et al. 2019

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Quantitative characterization of the tumor microenvironment, including its immuno-architecture, is important for developing quantitative diagnostic and predictive biomarkers, matching patients to the most appropriate treatments for precision medicine, and for providing quantitative data for building systems biology computational models able to predict tumor dynamics in the context of immune checkpoint blockade therapies. The intra- and inter-tumoral spatial heterogeneities are potentially key to the understanding of the dose-response relationships, but they also bring challenges to properly parameterizing and validating such models. In this study, we developed a workflow to detect CD8+ T cells from whole slide imaging data, and quantify the spatial heterogeneity using multiple metrics by applying spatial point pattern analysis and morphometric analysis. The results indicate a higher intra-tumoral heterogeneity compared with the heterogeneity across patients. By comparing the baseline metrics with PD-1 blockade treatment outcome, our results indicate that the number of high-density T cell clusters of both circular and elongated shapes are higher in patients who responded to the treatment. This methodology can be applied to quantitatively characterize the tumor microenvironment, including immuno-architecture, and its heterogeneity for different cancer types.

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Image Analysis in Surgical Pathology

Cited by 9 publications

References 33 publications

Tamoxifen and pregnancy: an absolute contraindication?

Tamoxifen and pregnancy: an absolute contraindication?

Poor outcome in hypoxic endometrial carcinoma is related to vascular density

Quantitative Characterization of CD8+ T Cell Clustering and Spatial Heterogeneity in Solid Tumors

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