Image Analysis in Microbiology: A Review

Pushchino,

doi:10.4236/jcc.2016.415002

Cited by 31 publications

(11 citation statements)

References 84 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Image analysis developments in other disciplines continue unabated, such as microbiology and sub-disciplines in engineering (e.g. Fredborg et al, 2015;Puchkov, 2016;Schindelin et al, 2012), and inter-disciplinary collaborations could offer novel opportunities. Detection of different species (e.g.…”

Section: Future Directionsmentioning

confidence: 99%

How do you find the green sheep? A critical review of the use of remotely sensed imagery to detect and count animals

et al. 2018

View full text Add to dashboard Cite

Animal abundance data are essential for endangered species conservation, tracking invasive species spread, biosecurity, agricultural applications and wildlife monitoring; however, obtaining abundance data are a perennial challenge. Recent improvements in the resolution of remotely sensed imagery, and image‐processing tools and software have facilitated improvement of methods for the detection of individual, generally large‐bodied animals. The potential to monitor and survey populations from remotely sensed imagery is an exciting new development in animal ecology. We review the methods used to analyse remotely sensed imagery for their potential to estimate the abundance of wild and domestic animal populations by directly detecting, identifying and counting individuals. Despite many illustrative studies using a variety of methods for detecting animals from remotely sensed imagery, it remains problematic in many situations. Studies that demonstrated reasonably high accuracy using automated and semi‐automated techniques have been undertaken on small spatial scales relative to the geographical range of the species of interest and/or in homogenous environments such as sea ice. The major limitations are the relatively low accuracy of automated detection techniques across large spatial extents, false detections and the cost of high‐resolution data. Future developments in the analysis of remotely sensed data for population surveys will improve detection capabilities, including the advancement of algorithms, the crossover of software and technology from other disciplines, and improved availability, accessibility, cost and resolution of data.

show abstract

Section: Future Directionsmentioning

confidence: 99%

How do you find the green sheep? A critical review of the use of remotely sensed imagery to detect and count animals

et al. 2018

View full text Add to dashboard Cite

show abstract

“…The overnight growth were then plated out on both blood and MacConkey agars, incubated aerobically at 37.5°C for 24 hours. The spread agar plate technique was used for counting of colonies while pure samples of the microbial cultures of all phenotypically identified colonies 23 , 24 were prepared and used for the identification of the Gram-negative bacteria isolates using the Vitek 2 Compact automated system (BioMerieux, Marcy-l’Etoile, France) according to manufacturer’s guidelines.…”

Section: Methodsmentioning

confidence: 99%

Assessment of Microbiological Content of Private and Public Recreational Water Facilities and Their Antimicrobial Susceptibility Pattern in Al-Ahsa

AlTuraifi

AlMomatin

Badger-Emeka

et al. 2019

Environ�Health�Insights

View full text Add to dashboard Cite

Background: Water recreational facilities like swimming pools attract people of all ages. However, these facilities are very suitable for the transmission of various microbial diseases and have been shown to pose public health concerns. Aims: This study assesses the presence of different Gram-negative bacteria pathogens and their antimicrobial susceptibility pattern in both private and public pools in Al-Ahsa. Methods: 11 private and 3 public recreational water facilities were sampled for the study. Collected water samples were inoculated into nutrient broth and incubated aerobically for 24 hours. The overnight growth was plated out on blood and MacConkey agars. Pure cultures of the bacteria samples were used for identification and antimicrobial susceptibility test using the Vitek 2 compactautomated system (BioMerieux, Marcy L’Etoile, France). Minimum inhibitory concentration was also provided by the Vitek 2 compact automated system. Results: 13 different Gram-negative bacteria species isolates were encountered in both pool types sampled. More of potential pathogens were isolated from the private than the public pools, of which Klebsiella pneumoniae and Pseudomonas aeruginosa constituted 50% and 43%, respectively, of all the isolates. Findings also revealed a varied minimum inhibitory concentrations (MICs) indicating that the isolates were of different strains. Antibiotic susceptibility pattern also showed variability among the isolates. Conclusions: This study has revealed a potential health risk associated with the use of water recreational facilities. The presence of K pneumoniae and P aeruginosa suggests a public health concern and should be looked into.

show abstract

“…One set of plates were incubated aerobically, whereas the other set anaerobically for 24 hours. Colony counts were performed using the spread agar plate method 24,25 with microbial colonies identified phenotypically. Basic bacteriological techniques were used for the preparation of pure colonies with the isolated bacteria identified with the Vitek 2 Compact automated system (BioMerieux) according to manufacturer's guidelines using the ID cards.…”

Section: Microbial Analysis Of Faecal Samplesmentioning

confidence: 99%

Vitamin D Supplementation in Laboratory-Bred Mice: An In Vivo Assay on Gut Microbiome and Body Weight

et al. 2020

View full text Add to dashboard Cite

Saudi Arabia is in a tropical geographical region with a population that has access to adequate diet. There is, however, a high level of vitamin D deficiency in the Kingdom, comorbid with other disease. There is the postulation of a correlation between a healthy gut microbiota and balanced levels of serum vitamin D. This investigation looks into the effect of vitamin D supplementation on the gut flora of laboratory-bred mice as well as any possible association on body weight. BALB/C mice weighing between 34 and 35.8 g were divided into 4 groups and placed on daily doses of vitamin D of 3.75 µg (low dose), 7.5 µg (normal dose), and 15 µg (high dose). The fourth group was the control group that did not receive any supplementation with vitamin D. Body weights were monitored on weekly basis, while faecal samples from the rectum were obtained for microbial culturing and the monitoring of bacterial colony count using the Vitek 2 Compact automated system (BioMerieux, Marcy-l’Etoile, France) according to manufacturer’s guidelines. The data presented as mean ± SD, while significant differences were determined with 2-way analysis of variance in comparing differences within and between treatment groups. The different doses of vitamin D showed varying effects on the body weight and gut microbial colonies of the mice. There was a highly significant difference between the control, 15 µg (high), and 7.5 µg (normal) dose groups. This is suggestive that supplementation with vitamin D could a role in the gut microbial flora in the gut which could reflect in changes in body weight.

show abstract

Image Analysis in Microbiology: A Review

Cited by 31 publications

References 84 publications

How do you find the green sheep? A critical review of the use of remotely sensed imagery to detect and count animals

How do you find the green sheep? A critical review of the use of remotely sensed imagery to detect and count animals

Assessment of Microbiological Content of Private and Public Recreational Water Facilities and Their Antimicrobial Susceptibility Pattern in Al-Ahsa

Vitamin D Supplementation in Laboratory-Bred Mice: An In Vivo Assay on Gut Microbiome and Body Weight

Contact Info

Product

Resources

About