Image acquisition and planimetry systems to develop wounding techniques in 3D wound model

Abstract

Wound healing represents a complex biological repair process. Established 2D monolayers and wounding techniques investigate cell migration, but do not represent coordinated multi-cellular systems. We aim to use wound surface area measurements obtained from image acquisition and planimetry systems to establish our wounding technique and in vitro organotypic tissue. These systems will be used in our future wound healing treatment studies to assess the rate of wound closure in response to wound healing treatment with light therapy (photobiomodulation). The image acquisition and planimetry systems were developed, calibrated, and verified to measure wound surface area in vitro. The system consists of a recording system (Sony DSC HX60, 20.4 M Pixel, 1/2.3'' CMOS sensor) and calibrated with 1mm scale paper. Macro photography with an optical zoom magnification of 2:1 achieves sufficient resolution to evaluate the 3mm wound size and healing growth. The camera system was leveled with an aluminum construction to ensure constant distance and orientation of the images. The JPG-format images were processed with a planimetry system in MATLAB. Edge detection enables definition of the wounded area. Wound area can be calculated with surface integrals. To separate the wounded area from the background, the image was filtered in several steps. Agar models, injured through several test persons with different levels of experience, were used as pilot data to test the planimetry software. These image acquisition and planimetry systems support the development of our wound healing research. The reproducibility of our wounding technique can be assessed by the variability in initial wound surface area. Also, wound healing treatment effects can be assessed by the change in rate of wound closure. These techniques represent the foundations of our wound model, wounding technique, and analysis systems in our ongoing studies in wound healing and therapy.

Automatic image analysis system to measure wound area in vitro

“…The previous work on wound area measurement was majorly focused on the image acquisition and planimetry system for developing wounding techniques, which was done in an agar model [2]. This work was followed by a method comparison study performed between the existing tolerance method and the proposed contour method for an in-vitro tissue model [3].…”

“…Our first work on the wound area measurement was made on digital images of the wounded agar model, through tolerance (contrast) edge detection followed by filtering the images [2]. The tolerance edge detection method was later compared with a contour edge detection method, which was performed on the digital images of a biological experimental setup.…”

Sistemas Computacionais Para Auxílio Na Cicatrização De Feridas: Revisão De Escopo

“…MowA®: A simple and economic way of monitoring chronic wounds outcome with your mobile devices Metodológico Plataforma mobile de uso profissional para análise de feridas e sugestões de tratamento Kiefer et al18 Image acquisition and planimetry systems to develop wounding techniques in 3D wound model Metodológico Plataforma web de uso profissional para mensuração de feridas e cálculo da área por imagens Seat et al19 A prospective trial of interrater and intrarater reliability of wound measurement using a smartphone app versus the traditional ruler…”