Iloprost binding and inhibition of aggregation in platelet rich plasma

Oliva, D; Maderna, P.; Accomazzo, Maria Rosa; Nicosia, S.; Tremoli, Elena

doi:10.1016/0006-2952(89)90146-9

Cited by 10 publications

(1 citation statement)

References 29 publications

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“…Iloprost is a stable prostacyclin analogue, and it promotes inhibition ofplatelet aggregation by raising endogenous cAMP (21)(22)(23). It was thus used to examine the effect of cAMP on thrombin-evoked alteration in platelet Na/H antiport parameters.…”

Section: Resultsmentioning

confidence: 99%

Cyclic nucleotides attenuate thrombin-evoked alterations in parameters of platelet Na/H antiport. The role of cytosolic Ca.

Kimura¹,

Lasker²,

Aviv³

1992

J. Clin. Invest.

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In this work, we explored the role of cyclic nucleotides in modulating parameters of the Na/H antiport in human platelets. Sodium nitroprusside and iloprost, as well as cyclic nucleotide analogues, were used to raise cellular levels of cAMP and cGMP. Cyclic nucleotides reversed the thrombin-evoked alkaline shift in cytosolic pH set point and the activity of the Na/H antiport, concurrently with attenuation of thrombin-induced rise in cytosolic free Ca. No effect of cyclic nucleotides was observed in platelets not treated with thrombin, or platelets subjected to phorbol 12-myristate 13-acetate. cAMP did not reverse ionomycin-induced changes in the parameters of the Na/H antiport. Collectively, these observations indicate that cyclic nucleotides modulate the Na/H antiporter in human platelets through their effect on thrombin-evoked changes in cytosolic free Ca. Presumably, this effect holds for other agonists which stimulate phospholipase C, raise cytosolic-free Ca, and activate the Na/H antiport through protein kinase C dependent and protein kinase C-independent mechanisms. (J.

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Section: Resultsmentioning

confidence: 99%