ILME: A Waterborne Pheromonal Peptide Released by the Eggs of Sepia officinalis

Zatylny, Céline; Gagnon, Jean; Boucaud‐Camou, E.; Henry, Joël

doi:10.1006/bbrc.2000.3286

Cited by 36 publications

(23 citation statements)

References 8 publications

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“…These factors thus suggest that mollusks may have developed defense strategies to protect themselves from exposure to a high density of microorganisms, including potential pathogens. The use of secondary metabolites is well documented in the phylum Mollusca as part of their communication systems (22)(23)(24), predatory behavior (25,26), and defensive secretions (27)(28)(29)(30). The abundance, diversity, and chemical potential of mollusks make this phylum of interest for studies of microbial symbiosis.…”

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confidence: 99%

Characterization of the Bacterial Community of the Chemically Defended Hawaiian Sacoglossan Elysia rufescens

Davis

Fricke

Hamann

et al. 2013

Appl Environ Microbiol

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dSacoglossans are characterized by the ability to sequester functional chloroplasts from their algal diet through a process called kleptoplasty, enabling them to photosynthesize. The bacterial diversity associated with sacoglossans is not well understood. In this study, we coupled traditional cultivation-based methods with 454 pyrosequencing to examine the bacterial communities of the chemically defended Hawaiian sacoglossan Elysia rufescens and its secreted mucus. E. rufescens contains a defense molecule, kahalalide F, that is possibly of bacterial origin and is of interest because of its antifungal and anticancer properties. Our results showed that there is a diverse bacterial assemblage associated with E. rufescens and its mucus, with secreted mucus harboring higher bacterial richness than entire-E. rufescens samples. The most-abundant bacterial groups affiliated with E. rufescens and its mucus are Mycoplasma spp. and Vibrio spp., respectively. Our analyses revealed that the Vibrio spp. that were highly represented in the cultivable assemblage were also abundant in the culture-independent community. Epifluorescence microscopy and matrix-assisted laser desorption-ionization mass spectrometry (MALDI-MS) were utilized to detect the chemical defense molecule kahalalide F on a longitudinal section of the sacoglossan.

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confidence: 99%

Characterization of the Bacterial Community of the Chemically Defended Hawaiian Sacoglossan Elysia rufescens

Davis

Fricke

Hamann

et al. 2013

Appl Environ Microbiol

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“…In this organism, several regulatory factors are involved in the successive steps of egg-laying. The release of a water-borne pheromonal peptide (ILME) by freshly spawned eggs induces the attraction of mature adults in egg-laying coastal areas (Zatylny et al, 2000). The presence of a specific hexapeptide amidated at its C terminus (PIDPGVamide), released in the external medium by the full-grown oocytes, can increase the probability of contact between gametes, thereby mobilizing the spermatozoa (Zatylny et al, 2002).…”

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confidence: 99%

Sperm-attractant peptide influences the spermatozoa swimming behavior in internal fertilization in Octopus vulgaris

Lisa

Salzano

Moccia

et al. 2013

Journal of Experimental Biology

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SUMMARYMarine invertebrates exhibit both chemokinesis and chemotaxis phenomena, induced in most cases by the release of water-borne peptides or pheromones. In mollusks, several peptides released during egg-laying improve both male attraction and mating. Unlike other cephalopods, Octopus vulgaris adopts an indirect internal fertilization strategy. We here report on the identification and characterization of a chemoattractant peptide isolated from mature eggs of octopus females. Using two-chamber and timelapse microscopy assays, we demonstrate that this bioactive peptide is able to increase sperm motility and induce chemotaxis by changing the octopus spermatozoa swimming behavior in a dose-dependent manner. We also provide evidence that chemotaxis in the octopus requires the presence of extracellular calcium and membrane protein phophorylation at tyrosine. This study is the first report on a sperm-activating factor in a non-free-spawning marine animal.

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“…The first ovarian regulatory peptide ever characterized was the tetrapeptide ILME [4], followed by SepOvotropin [5], SepCRPs (Sepia Capsule Releasing Peptides) [6,8], and OJPs (Ovarian Jelly Peptides) [7]. All these peptides modulate the contraction of the distal oviduct, and some of them also regulate the contraction of the main nidamental glands (Figures 7B, C, and 8A-E).…”

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confidence: 99%

“…In SepCRPs, there was a mistake about the Figure 7. Perfusion of distal oviduct with (A) seawater used for incubating mature oocytes (SWO), (B) the synthetic peptide ILME and (C) synthetic SepOvotropin [4,5]. amino acid in position 5: aspartate (D) should be replaced by asparagine (N) (EISLNKDEVK instead of EISLNKD).…”

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Egg-Laying in the Cuttlefish Sepia officinalis

Zatylny-Gaudin¹,

Henry²

2018

Biological Resources of Water

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This chapter reviews studies about egg-laying in the cuttlefish Sepia officinalis. Egg masses are spawned in specific mating and spawning coastal areas where mates aggregate between April and June in the English Channel and all year long in the Mediterranean Sea. Environmental cues are clearly involved in the aggregation process, but chemical communication also plays a determining role in these complex mechanisms. The successive steps of egg-laying are orchestrated by three classes of regulatory peptides: (1) neuropeptides that integrate environmental cues, (2) ovarian regulatory peptides that modulate the activity of the genital tract, and (3) sex pheromones expressed and released by the oviduct gland. After egg-laying, embryo protection is ensured for 8-10 weeks by a multilayer capsule secreted by the accessory sex glands. The oviduct gland secretes the inner layer of the egg case. The main nidamental gland secretes the main polysaccharides and glycoproteins, such as Sepia Egg Case Proteins, involved in capsule formation and in embryo protection. The accessory nidamental gland expresses specific proteins inherent in the structural organization of the gland, and hosts symbiotic bacteria. Similarly to salivary glands, this gland secretes immune factors possibly associated with gamete and/or embryo protection.

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ILME: A Waterborne Pheromonal Peptide Released by the Eggs of Sepia officinalis

Cited by 36 publications

References 8 publications

Characterization of the Bacterial Community of the Chemically Defended Hawaiian Sacoglossan Elysia rufescens

Characterization of the Bacterial Community of the Chemically Defended Hawaiian Sacoglossan Elysia rufescens

Sperm-attractant peptide influences the spermatozoa swimming behavior in internal fertilization in Octopus vulgaris

Egg-Laying in the Cuttlefish Sepia officinalis

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