Illumina MiSeq Sequencing Reveals Correlations among Fruit Ingredients, Environmental Factors, and AMF Communities in Three
            <i>Lycium Barbarum</i>
            Producing Regions of China

Chen, Kaili; Huang, Gang; Li, Yuekun; Zhang, Xinrui; Lei, Yonghui; Li, Yang; Xiong, Jie; Sun, Yanfei

doi:10.1128/spectrum.02293-21

Cited by 14 publications

(12 citation statements)

References 72 publications

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“…In addition, the fungal community also affects the growth and quality of its host. Chen et al (2022) revealed the fungal community of arbuscular mycorrhizal fungi (AMF) in rhizosphere soils of L. barbarum through Illumina Miseq platform and demonstrated that three fungal genera ( Claroideoglomus , Dominikia and Funneliformis ) were related to LF fruit ingredients. Additionally, AMF also affected the tolerance ability of LF to drought stress.…”

Section: Discussionmentioning

confidence: 99%

Investigation of fungal contamination in medicinal and edible Lycii Fructus through DNA metabarcoding

Jiang

Guo

et al. 2022

Journal of Applied Microbiology

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Section: Discussionmentioning

confidence: 99%

Investigation of fungal contamination in medicinal and edible Lycii Fructus through DNA metabarcoding

Jiang

Guo

et al. 2022

Journal of Applied Microbiology

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“…However, studies have shown that nutrient addition caused decreases in AMF diversity and abundance [27,48,49]. Corresponding changes in soil factors have different degrees of impact on AMF community and diversity [49][50][51]. Differences in concentrations of nutritional factors may lead to the loss of some fungal species and promote the dominance of certain AMF species [52].…”

Section: Discussionmentioning

confidence: 99%

Arbuscular Mycorrhizal Fungi Associated with Roots Reveal High Diversity Levels at Different Elevations in Tropical Montane Rainforests

Zhang

Shi

et al. 2022

Diversity

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(1) Background: Understanding the diversity of communities of arbuscular mycorrhizal fungi (AMF) is the basis for understanding the ecological functions of AMF. (2) Methods: The community diversity and distribution of AMF at different elevations in tropical montane rainforests of Mt. Jianfenfling and Mt. Diaoluo were explored using high-throughput sequencing technology. (3) Results: A total of 283 AMF operational taxonomic units (OTUs) were identified from roots and the number of unique OTUs was 173, accounting for 61.13% of the total number discovered in these tropical montane rainforests. At different altitudes, high turnovers of AMF were observed, with the maximum proportion of unique OTUs between two altitudes being 45.16%, recorded between a.s.l. 250 m and 900 m on Mt. Diaoluo. The highest Sobs, Shannon and Pielou diversity indices appeared at 650 m on Mt. Diaoluo. For the two mountains, the soil properties of C, N and C/N have significant impacts on the genera Scutellospora, Paraglomus and unclassified in Archaeosporaceae, while the genera Glomus, Diversispora and Acaulospora are significantly affected by soil P and pH. It can be considered that altitude probably determines the presence of AMF communities by affecting edaphic properties. (4) Conclusions: There are abundant AMF associated with roots in the tropical montane rainforests of China. Furthermore, a high turnover of OTUs was found to exist between the mountains and at different altitudes, revealing diverse AMF community structures in tropical montane rainforests.

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“…PCR reactions were performed as previously described ( 30 ). Purified PCR products were analyzed using the Illumina Miseq PE300 sequencing platform (Illumina, United States) ( 31 ). All sequencing data have been deposited in the NCBI Sequence Read Archive (SRA) under accession number PRJNA781802 .…”

Section: Methodsmentioning

confidence: 99%

Bacillus velezensis EEAM 10B Strengthens Nutrient Metabolic Process in Black Soldier Fly Larvae (Hermetia illucens) via Changing Gut Microbiome and Metabolic Pathways

et al. 2022

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Insects are a potential alternative protein source to solve the food shortage crisis. Previous studies have illustrated that probiotics can improve the substrate conversion efficiency of insects and increase insect protein content. However, the effects of probiotics on insect physiology and nutrient metabolism are still not well understood. Here, the black soldier fly larvae (BSFL), Hermetia illucens (Diptera: Stratiomyidae), was used as a study subject to deeply investigate the specific interaction among a novel probiotic, Bacillus velezensis EEAM 10B (10B), intestinal microbiota, and the host. In this study, the effects of 10B on the survival and physiology of BSFL were first analyzed. It shows that 10B significantly elevated the substrate conversion rate, average dry weight, and protein content of BSFL by 5%, 0.13 g/pc, and 8%, respectively. Then, we assessed the effect of 10B on the microbial community composition in the gut and frass of BSFL using Illumina Miseq sequencing. It shows that 10B significantly altered the microbial composition of the gut, but not that of the frass. Pearson’s correlation analysis further showed that the Bacillus, unclassified_of_Caloramatoraceae, and Gracilibacillus were positively correlated with the survival rate, crude protein content, and substrate conversion rate of BSFL. To further investigate the effect of 10B on host metabolism, metabolic analyses on germ-free BSFL, monobacterial intestinal BSFL, and natural BSFL were also performed. The results proved that 10B (i) played a vital role in the survival of BSFL; and (ii) regulated the amino acid synthetic and metabolic process of BSFL, thus leading to the rise of the protein content of BSFL. In addition, vitamin backfill assays verified that the BSFL survival rate was significantly improved by supplying the germ-free BSFL with riboflavin, which further suggests that 10B determines the survival of BSFL via delivering riboflavin. Overall, this study provides a reference for understanding the comprehensive contribution of a specific probiotic to its host.

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Illumina MiSeq Sequencing Reveals Correlations among Fruit Ingredients, Environmental Factors, and AMF Communities in Three Lycium Barbarum Producing Regions of China

Cited by 14 publications

References 72 publications

Investigation of fungal contamination in medicinal and edible Lycii Fructus through DNA metabarcoding

Investigation of fungal contamination in medicinal and edible Lycii Fructus through DNA metabarcoding

Arbuscular Mycorrhizal Fungi Associated with Roots Reveal High Diversity Levels at Different Elevations in Tropical Montane Rainforests

Bacillus velezensis EEAM 10B Strengthens Nutrient Metabolic Process in Black Soldier Fly Larvae (Hermetia illucens) via Changing Gut Microbiome and Metabolic Pathways

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