Ileal Digestibility of Nitrogen and Amino Acids in Human Milk and an Infant Formula as Determined in Neonatal Minipiglets

Charton, Elise; Henry, Gwénaële; Cahu, Armelle; Le-Gouar, Yann; Dahirel, Patrice; Moughan, Paul J.; Montoya, Carlos A.; Bellanger, Amandine; Dupont, Didier; Huërou-Luron, Isabelle Le; Deglaire, Amélie

doi:10.1016/j.tjnut.2023.02.025

Cited by 4 publications

(4 citation statements)

References 53 publications

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“…The digestibility of lysine was numerically lower for Tþþþ than for T− but this did not reach statistical significance. A similar result was observed when comparing highly heat-treated infant formula and native human milk (34) . The determination of reactive lysine could have provided more information regarding lysine digestibility values.…”

Section: Discussionsupporting

confidence: 83%

“…These values are consistent with those reported in the literature for milk proteins in rat studies (14,24,32) . Slightly higher nitrogen and amino acid true ileal digestibility values (98 %) of the heat-treated infant formula Tþþþ have been reported in piglets (34) , which might be due to the animal model. Despite the fact that no significant difference was reported, orocaecal nitrogen and amino acid true digestibility of the Tþþþ diet tended to be lower than for the T− diet.…”

Section: Discussionmentioning

confidence: 95%

“…Moreover, the values obtained for the DIAAS were in line with previously reported ones. Tþþþ infant formula displayed a DIAAS of 0•83 (for aromatic amino acids) when assessed in piglets (34) and an in vitro dynamic model simulating infant digestion reported DIAAS values of 0•75 (for aromatic amino acids) for an infant formula (39) . A small decrease in lysine content was observed in infant formulas when considering total or reactive lysine (0-0•3 g/100 g of protein) (38) .…”

Section: Discussionmentioning

confidence: 99%

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Minimal processed infant formula vs. conventional shows comparable protein quality and increased postprandial plasma amino acid kinetics in rats

Calvez,

Blais,

Deglaire

et al. 2023

Br J Nutr

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During industrial processing, heat treatments applied to infant formulas may affect protein digestion. Recently, innovative processing routes have been developed to produce minimally heat-processed infant formula. Our objective was to compare the in vivo protein digestion kinetics and protein quality of a minimally processed (T−) and a heat-treated (T+++) infant formula. Sixty-eight male Wistar rats (21 d) were fed with either a diet containing 40 % T− (n 30) or T+++ (n 30), or a milk protein control diet (n 8) during 2 weeks. T− and T+++ rats were then sequentially euthanised 0, 1, 2, 3 or 6 h (n 6/time point) after ingestion of a meal containing their experimental diet. Control rats were euthanised 6 h after ingestion of a protein-free meal to determine nitrogen and amino acid endogenous losses. Nitrogen and amino acid true caecal digestibility was high for both T− and T+++ diets (> 90 %), but a tendency towards higher nitrogen digestibility was observed for the T− diet (96·6 ± 3·1 %) compared with the T+++ diet (91·9 ± 5·4 %, P = 0·0891). This slightly increased digestibility led to a greater increase in total amino acid concentration in plasma after ingestion of the T− diet (P = 0·0010). Comparable protein quality between the two infant formulas was found with a digestible indispensable amino acid score of 0·8. In conclusion, this study showed that minimal processing routes to produce native infant formula do not modify protein quality but tend to enhance its true nitrogen digestibility and increase postprandial plasma amino acid kinetics in rats.

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Section: Discussionsupporting

confidence: 83%

Section: Discussionmentioning

confidence: 95%

Section: Discussionmentioning

confidence: 99%

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Minimal processed infant formula vs. conventional shows comparable protein quality and increased postprandial plasma amino acid kinetics in rats

Calvez,

Blais,

Deglaire

et al. 2023

Br J Nutr

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“…A study using mini-piglet as a model demonstrated that the digestibility of individual AAs in HM and IF was similar at the ileal terminal, except for threonine. 44 Accepted manuscript digestive tract. 45,46 In the future, such techniques will likely elucidate a more detailed understanding of the in vivo digestive dynamics of HM and IF in infants.…”

Section: Discussionmentioning

confidence: 99%

Comparison of protein digestibility of human milk and infant formula using the INFOGEST method under infant digestion conditions

Komatsu,

Wada,

Shibasaki

et al. 2024

Br J Nutr

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Many improvements have been made to bring infant formula (IF) closer to human milk (HM) regarding its nutritional and biological properties. Nevertheless, the protein components of HM and IF are still different, which may affect their digestibility. This study aimed to evaluate and compare the protein digestibility of HM and IF using the infant INFOGEST digestion method. Pooled HM and a commercial IF were subjected to the infant INFOGEST method, which simulates the physiological digestion conditions of infants, with multiple directions, i.e., the curd state, SDS-PAGE, molecular weight distribution, free amino acid concentration, and in vitro protein digestion rate. HM underwent proteolysis before digestion, and tended to have a higher protein digestion rate with finer curds during gastric digestion, than the IF. However, multifaceted analyses showed that the protein digestibility of HM and IF was not significantly different after gastrointestinal digestion. In conclusion, the infant INFOGEST method showed that the digestibility of HM and IF proteins differed to some extent before digestion and after gastric digestion, but not at the end of gastrointestinal digestion. The findings of this study will contribute to the refinement of IFs with better protein digestibility in infant stomach.

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Towards more biomimetic and sustainable infant formula: challenges and future opportunities

Deglaire

Lee

Lanotte

et al. 2023

Trends in Food Science & Technology

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Ileal Digestibility of Nitrogen and Amino Acids in Human Milk and an Infant Formula as Determined in Neonatal Minipiglets

Cited by 4 publications

References 53 publications

Minimal processed infant formula vs. conventional shows comparable protein quality and increased postprandial plasma amino acid kinetics in rats

Minimal processed infant formula vs. conventional shows comparable protein quality and increased postprandial plasma amino acid kinetics in rats

Comparison of protein digestibility of human milk and infant formula using the INFOGEST method under infant digestion conditions

Towards more biomimetic and sustainable infant formula: challenges and future opportunities

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