IL28B polymorphisms predict the response to chronic hepatitis C virus infection treatment in a Mexican population

Martínez-Gómez, Laura E.; Chávez-Tapia, Norberto C.; Burguete-García, Ana I.; Aguilar-Olivos, Nancy E.; Madrid‐Marina, Vicente; Román-Bahena, Margarita; Orbe-Orihuela, Citlalli; Uribe, Misael; Méndez-Sánchez, Nahúm

doi:10.1016/s1665-2681(19)31413-9

Cited by 15 publications

(8 citation statements)

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“…On the other hand, no association was found with the rs8099917TT genotype and SC. This result is consistent with a report in Chinese patients [ 35 ] and with one previous study in Central Mexico that found no association with the response to pegIFN-α/RBV therapy [ 32 ]. In concordance with these observations, in this study the C/T/TT haplotype was associated with SC and less liver damage than those with the risk haplotype.…”

Section: Discussionsupporting

confidence: 93%

Association with Spontaneous Hepatitis C Viral Clearance and Genetic Differentiation of IL28B/IFNL4 Haplotypes in Populations from Mexico

et al. 2016

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AimTo analyze the genetic heterogeneity of the Amerindian and admixed population (Mestizos) based on the IL28B (rs12979860, rs8099917) and IFNL4 (rs368234815) haplotypes, and their association with spontaneous clearance (SC) and liver damage in patients with hepatitis C infection from West Mexico.MethodsA total of 711 subjects from West Mexico (181 Amerindians and 530 Mestizos) were studied for the prevalence of IL28B (rs12979860C/T, rs8099917G/T) and IFNL4 (rs368234815∆G/TT) genotypes. A case-control study was performed in 234 treatment-naïve HCV Mestizos (149 chronic hepatitis C and 85 with SC) for the association of haplotypes with SC and liver damage. A real-time PCR assay was used for genotyping, and transitional elastography staged liver damage.ResultsSignificant Fst-values indicated differentiation between the studied populations. The frequencies of the protective C, T, TT alleles were significantly lower in the Amerindians than in Mestizos (p<0.05). The r2 measure of linkage disequilibrium was significant for all variants and the T/G/ΔG risk haplotype predominated in Amerindians and secondly in Mestizos. The protective C/T/TT haplotype was associated with SC (OR = 0.46, 95% IC 0.22–0.95, p = 0.03) and less liver damage (OR = 0.32, 95% IC 0.10–0.97, p = 0.04) in chronic patients. The Structure software analysis demonstrated no significant differences in ancestry among SC and chronic patients.ConclusionsWest Mexico´s population is genetically heterogeneous at the IL28B/IFNL4 polymorphisms. The T/G/ΔG high-risk haplotype predominated in Amerindians and the beneficial alternative haplotype in Mestizos. The C/T/TT haplotype was associated with SC and less liver damage in chronically infected Mestizo patients.

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Section: Discussionsupporting

confidence: 93%

Association with Spontaneous Hepatitis C Viral Clearance and Genetic Differentiation of IL28B/IFNL4 Haplotypes in Populations from Mexico

et al. 2016

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“…In countries such as Iran, the analysis of strategies based on IL28B rs12979860 and SVR could be useful in terms of cost-effectiveness. This analysis could also provide benefits related to adverse effects, treatment tolerance, and induction of viral drug resistance [26] .…”

Section: Discussionmentioning

confidence: 99%

Comparison of Three Different Methods for Detection of IL28 rs12979860 Polymorphisms as a Predictor of Treatment Outcome in Patients with Hepatitis C Virus

Fateh

Aghasadeghi

Siadat

et al. 2016

Osong Public Health and Research Perspectives

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ObjectivesThis study aimed to evaluate the specificity, sensitivity, cost, and turn-around time of three methods of gene polymorphism analysis and to study the relationship between IL28B rs12979860 and SVR rate to pegIFN-α/RVB therapy among patients with chronic hepatitis C.MethodsA total of 100 samples from chronic hepatitis C patients were analyzed in parallel using the three methods: direct sequencing, real-time polymerase chain reaction (PCR), amplification refractory mutation system (ARMS)-PCR.ResultsThe different profiles for IL28B rs12979860 alleles (CC, CT, and TT) obtained with PCR-RFLP, ARMS-PCR, and direct sequencing were consistent among the three methods. Prevalence of rs12979860 genotypes CC, CT and TT in HCV genotype 1a was 10(19.6%), 35(68.6%), and six (11.8%), respectively, and in HCV genotype 31, it was 13(26.5%), 31(63.3%), and five (10.2%), respectively. No significant difference was seen between rs12979860 genotype and HCV genotype (p = 0.710).ConclusionScreening by ARMS – PCR SNOP detection represents the most efficient and reliable method to determine HCV polymorphisms in routine clinical practice.

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“…Several reports establish a strong link between SNPs rs12979860 and rs8099917 with the response to peg-IFN-α/RBV treatment for HCV genotype 1 infected patients [ 7 , 9 , 13 , 25 , 28 , 29 , 31 , 37 – 39 ]. To date no study exists that shows the distribution of these polymorphisms within the Uruguayan population despite the fact that HCV genotype 1 is confirmed as the most prevalent viral genotype in the country (Table 1 ) [ 40 ].…”

Section: Resultsmentioning

confidence: 99%

“…In this context, Asians and Europeans are better responders to peg-IFN-α/RBV treatment than African-descendants [ 22 ]. The case for Latin America is less clear as the prevalence of these IL28B SNPs is known only in a few countries [ 25 – 31 ]. Furthermore, given the admixed genetic background of the Latin American populations, it is unreasonable to assume an equivalent distribution of IL28B SNPs in all South America.…”

Section: Introductionmentioning

confidence: 99%

IL28B gene polymorphism rs12979860, but not rs8099917, contributes to the occurrence of chronic HCV infection in Uruguayan patients

Echeverría

Chiodi

López

et al. 2018

Virol J

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BackgroundHost single-nucleotide polymorphisms (SNPs) near the interleukin 28B (IL28B) locus are associated with sustained virological response to antiviral therapy and with spontaneous Hepatitis C Virus (HCV) clearance. Prevalence of these SNPs varies depending on ethnicity. The impact of IL28B SNPs in HCV-infected patients is currently unknown in Uruguay. Therefore, the aim of this study was to evaluate and compare the distribution of polymorphisms in the IL28B gene (rs12979860 and rs8099917) among HCV-infected patients and healthy individuals in Uruguay and thus assess their possible association with the establishment of HCV infection.MethodsDNA was recovered from 92 non-infected individuals and 78 HCV-infected patients and SNPs were determined by RFLP and allelic discrimination by real-time PCR.ResultsThe distribution of rs12979860 genotypes for the infected population was 29.5%-CC, 47.4%-CT and 23.1%-TT and for the control group 45.7%, 42.4% and 11.9%, respectively. Prevalence in both infected and uninfected individuals is similar to that reported in other countries with admixed populations. The distribution of rs8099917 genotypes for the infected population was 57.7%-TT, 27.2%-TG and 14.1%-GG and for the control group 60.9%, 33.7% and 5.4%, respectively. The comparison of rs12979860 genotype distribution between the two populations evidenced a higher prevalence of the favourable genotype (CC) in the uninfected control group (p < 0.05). Additionally, results generated using logistic regression analysis show that individuals carrying rs12979860-TT or CT genotypes have a higher likelihood of developing chronic hepatitis upon infection with HCV, when compared to CC carriers, considering rs8099917 genotype as constant.ConclusionPatients with HCV infection have a statistically significant lower prevalence of the favourable rs12979860 genotype when compared to uninfected individuals; therefore we can establish that only IL28B rs12979860-CT and TT genotypes seem to contribute to the occurrence of chronic HCV infection in the cohort of Uruguayan population studied. Considering that a trend towards a higher frequency of “good” response genotypes was observed in responder patients, we believe that IL28B rs12979860 genotyping could be a useful tool for predicting different therapies outcome, including in the DAA era.Electronic supplementary materialThe online version of this article (10.1186/s12985-018-0946-2) contains supplementary material, which is available to authorized users.

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IL28B polymorphisms predict the response to chronic hepatitis C virus infection treatment in a Mexican population

Cited by 15 publications

References 22 publications

Association with Spontaneous Hepatitis C Viral Clearance and Genetic Differentiation of IL28B/IFNL4 Haplotypes in Populations from Mexico

Association with Spontaneous Hepatitis C Viral Clearance and Genetic Differentiation of IL28B/IFNL4 Haplotypes in Populations from Mexico

Comparison of Three Different Methods for Detection of IL28 rs12979860 Polymorphisms as a Predictor of Treatment Outcome in Patients with Hepatitis C Virus

IL28B gene polymorphism rs12979860, but not rs8099917, contributes to the occurrence of chronic HCV infection in Uruguayan patients

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