IL2‐inducible T‐cell kinase inhibitor ibrutinib reduces symptoms and Th2 differentiation in mouse allergic‐rhinitis model

Xu, Bing; Liu, Xiaozhe; Gao, Shi-Hao

doi:10.1002/ddr.21884

Cited by 4 publications

(5 citation statements)

References 30 publications

(32 reference statements)

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“…Studies had shown that the level of IL7R in sepsis was significantly reduced, which was consistent with our results [ 54 ]. ITK is a member of the Tec family tyrosine kinases and mediates T cell receptor (TCR) signaling pathway [ 55 ]. Nadeem A proposed that ITK signaling plays a significant role in sepsis-induced acute lung injury [ 56 ].…”

Section: Discussionmentioning

confidence: 99%

Screening of Sepsis Biomarkers Based on Bioinformatics Data Analysis

Liang

et al. 2022

Journal of Healthcare Engineering

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Background and objectives. Sepsis is a life-threatening organ dysfunction caused by the imbalance of the body’s response to infection. Delay in sepsis diagnosis has become a primary cause of patient death. This study aims to identify potential biomarkers of sepsis based on bioinformatics data analysis, so as to provide new gene biomarkers for the diagnosis and treatment of sepsis. Methods. Gene expression profiles of GSE13904, GSE26378, GSE26440, GSE65682, and GSE69528 were obtained from the National Center for Biotechnology Information (NCBI). The differentially expressed genes (DEGs) were searched using limma software package. Gene Ontology (GO) functional analysis, Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment analysis, and protein-protein interaction (PPI) network analysis were performed to elucidate molecular mechanisms of DEGs and screen hub genes. Results. A total of 108 DEGs were identified in the study, of which 67 were upregulated and 41 were downregulated. 15 superlative diagnostic biomarkers (CCL5, CCR7, CD2, CD27, CD274, CD3D, GNLY, GZMA, GZMH, GZMK, IL2RB, IL7R, ITK, KLRB1, and PRF1) for sepsis were identified by bioinformatics analysis. Conclusion. 15 hub genes (CCL5, CCR7, CD2, CD27, CD274, CD3D, GNLY, GZMA, GZMH, GZMK, IL2RB, IL7R, ITK, KLRB1, and PRF1) have been elucidated in this study, and these biomarkers may be helpful in the diagnosis and therapy of patients with sepsis.

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Section: Discussionmentioning

confidence: 99%

Screening of Sepsis Biomarkers Based on Bioinformatics Data Analysis

Liang

et al. 2022

Journal of Healthcare Engineering

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“…Eight-week-old female BALB/c mice were provided via Charles River Laboratories (Beijing, China), and housed in a climate-controlled environment. The murine AR model was established as previously reported [24, 25]. In brief, 24 mice were randomly divided into 4 groups (control, AR, AR + pc-NC, or AR + pc-circARF3 group; n = 6 per group).…”

Section: Methodsmentioning

confidence: 99%

“…House dust mite (HDM) is a common allergen that has been utilized extensively to induce allergic inflammation in AR mice [24, 25]. Our study established an AR model using HDM-treated BALB/c mice and IL-4/IL-13-stimulated mice NEpCs.…”

Section: Introductionmentioning

confidence: 99%

CircARF3 Mitigates Allergic Rhinitis through Targeting microRNA-205-5p/Sirtuin 5 Axis

Zheng

Chen

Zhan

et al. 2023

Int Arch Allergy Immunol

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Introduction: Circular RNAs (circRNAs) are essential in the progression of allergic rhinitis (AR). The purpose of this research was to examine the role of circRNA ADP-ribosylation factor 3 (circARF3) in the pathogenesis of AR. Methods: To generate an animal model of AR, mice were treated with house dust mite (HDM), and mice nasal epithelial cells (NEpCs) were treated with IL-4/IL-13 to imitate the inflammatory damage of AR in vitro. Sanger sequencing, qRT-PCR, and RNAse R digestion assays all validated the circularization structure of circARF3. The levels of circARF3, miR-205-5p, and sirtuin 5 (SIRT5) were determined by qRT-PCR or Western blotting. Luciferase reporter, RNA immunoprecipitation, and pull-down experiments were used to investigate the regulatory network. Flow cytometry was used to investigate the rate of cell apoptosis, and Western blotting was used to determine the levels of apoptotic-related proteins (cleaved caspase 3, cleaved polyadenosine-diphosphate-ribose polymerase) and HMGB1, TLR4, and MyD88. Enzyme-linked immunosorbent assay was used to assess the inflammatory response. Hematoxylin-eosin staining and TUNEL were used to detect the histology of injury and apoptosis of nasal mucosa tissues. Results: CircARF3 and SIRT5 levels were reduced in HDM-treated animals and IL-4/IL-13-treated NEpCs, while miR-205-5p expression was increased. CircARF3 was generated by back-splicing exons 3–5 with a stable circular shape. CircARF3 overexpression mitigated IL-4/IL-13-induced apoptosis in NEpCs by inhibiting miR-205-5p. SIRT5 upregulation attenuated IL-4/IL-13-induced inflammatory injury in NEpCs, and SIRT5 knockdown induced opposite effects. miR-205-5p silencing reversed the effects of SIRT5 knockdown on IL-4/IL-13-induced inflammatory injury. Furthermore, circARF3 overexpression alleviated histological abnormalities, apoptosis, inflammatory response, and HMGB1/TLR4 signaling activation in HDM-treated animals. Conclusion: CircARF3 inhibited cell apoptosis and inflammation via the miR-205-5p/SIRT5 axis in IL-4/IL-13-treated NEpCs and HDM-treated mice.

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“…qRT-PCR was performed as described previously. 18 Briefly, total RNA from cells was extracted using PureLink RNA Mini Kit (Thermo Fisher Scientific Corp.) and then reverse-transcribed to cDNA using a high-capacity cDNA reverse transcription kit (Thermo Fisher Scientific Corp.). Real-time PCR was performed with PowerUp SYBR Green Master Mix and performed using QuantStudio 5 Real-Time PCR System (Thermo Fisher Scientific Corp.).…”

Section: Methodsmentioning

confidence: 99%

“…Western blotting was performed following a standard protocol described previously. 18 Briefly, total proteins were extracted from cells by RIPA lysis buffer (Abcam, Cambridge, UK) and protein concentration was measured using a bicinchoninic acid (BCA) protein assay kit (Abcam). Equal amounts of protein (25 µg) resolved by SDS-PAGE and transferred to polyvinylidene difluoride (PVDF) membranes.…”

Section: Methodsmentioning

confidence: 99%

IL2-inducible T-cell Kinase is Required for HBV-induced Type T Interferon Expression and Antiviral Response

Lin¹,

Guo²,

Zeng³

et al. 2023

J Clin Transl Hepatol

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Background and Aims: Hepatitis B is a vaccine-preventable liver infection caused by the hepatitis B virus (HBV), and is seen as a serious global health problem. HBV infection induces the expression of type I interferon (IFN), including IFN-α and IFN-β, which have anti-HBV activities, and have been used for HBV treatment. IL2-inducible T-cell kinase (ITK) is a tyrosine kinase, which regulates T-cell differentiation and activation, while its precise effects on type I IFN production during HBV infection remain unknown. Methods: We moni-

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IL2‐inducible T‐cell kinase inhibitor ibrutinib reduces symptoms and Th2 differentiation in mouse allergic‐rhinitis model

Cited by 4 publications

References 30 publications

Screening of Sepsis Biomarkers Based on Bioinformatics Data Analysis

Screening of Sepsis Biomarkers Based on Bioinformatics Data Analysis

CircARF3 Mitigates Allergic Rhinitis through Targeting microRNA-205-5p/Sirtuin 5 Axis

IL2-inducible T-cell Kinase is Required for HBV-induced Type T Interferon Expression and Antiviral Response

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