IL17A and IL17F genes polymorphisms are associated with histopathological changes in transplanted kidney

Abstract

Background Interleukin 17 is a proinflammatory cytokine involved in immune response after allograft transplantation. IL-17 family of proinflammatory cytokines includes IL-17A and IL-17F . Previous studies have demonstrated that the rs2275913 IL17A and the rs11465553 IL17F gene polymorphism are associated with kidney allograft function. Because of the association between these polymorphisms and post-transplant immune response, we assume that these single nucleotide polymorphisms may affect morphological structure of transplanted kidney. The aim of this study was to examine the association of rs2275913 IL17A and rs2397084, rs11465553 and rs763780 IL17F gene polymorphisms with histopathological changes in transplanted kidney biopsies such as: glomerulitis, tubulitis, arteritis, cell infilitration and fibrosis. Methods The study enrolled 82 patients after renal graft transplantation in whom a kidney biopsy was performed because of impaired graft function. The rs2397084 T > C (Glu126Gly), rs11465553 G > A (Val155Ile) and rs763780 T > C (His167Arg) polymorphisms within the IL17F gene and the rs2275913 A > G (− 197 A > G) polymorphism within the IL17A gene promoter were genotyped using TaqMan genotyping assays on a 7500 FAST Real-Time PCR System (Applied Biosystems, USA). Results There was a significant association between the rs2275913 IL17A gene polymorphism and the grade of tubulitis, which was more severe among patients with the A allele, compared to recipients with the GG genotype (GG vs. AG + AA, P = 0.02), and with the grade of arteriolar hyaline thickening and mesangial matrix increase, which were more severe among patients with the G allele compared to recipients with the AA genotype (AA vs. AG + GG, P = 0.01 and P = 0.04, respectively). Tubular atrophy and interstitial fibrosis were more severe among individuals with the C allele at the rs763780 IL17F gene polymorphism (TT vs. TC, P = 0.09 and P = 0.017, respectively). However, it should be taken into account that the statistical significance was achieved without correction for multiple testing, and no significant association would remain significant after such correction. Conclusions The results of this study may suggest a possible association between the rs2275913 IL17A and rs2275913 IL17A gene polymorphisms and some histopathological changes in transplanted kidney biopsies. Electronic supplementary material The online version of this article (10.1186/s12882-019-13...

IL-17 gene polymorphism (rs763780) in kidney recipients with post-transplant diabetes

“…(22), since in the present study, no significant association between NODAT and CC and TC genotypes was seen. In previous studies, the C17 allele of the IL-17F rs76378 gene polymorphism was associated with high concentrations of long-term creatinine and high severity of tubular atrophy and interstitial fibrosis were reported (21). However, in the present study, no significant difference was observed between the distribution of genotypes of the IL-17F T/C (rs763780) and the amount of creatinine and FBS three months and six months after transplantation.…”

“…IL-17F gene SNP 7489A/G is linked with graft failure (20). Moreover, the association of IL-17A rs2275913 gene polymorphism with histopathological changes of kidney allograft (21) and its association (rs2275913 GG) with an increased risk of delayed graft function and higher serum creatinine have been reported (22). The GA genotype of the IL-17F gene polymorphism (rs11465553) might be associated with a high risk of transplanted renal function and return to dialysis after kidney transplantation (22).…”

IL-17A polymorphism (rs2275913) and levels are associated with preeclampsia pathogenesis in Chinese patients

“…Common polymorphisms in the IL-17A gene have been associated with hypertension [ 31 ] and various organ dysfunctions [ 32 ]. As the primary clinical characteristics of PE are high blood pressure and dysfunction of kidney and liver, we hypothesized that variants in the IL-17A gene would be associated with predisposition to the development of PE.…”

IL-17A Polymorphism (rs2275913) and Levels Are Associated With Preeclampsia Pathogenesis in Chinese Patients  

“…Common polymorphisms in the IL-17A gene have been associated with hypertension (Huang et al 2017)and various organ dysfunctions (Domanski et al 2019). As the primary clinical characteristics of PE are high blood pressure and dysfunction of kidney and liver, we hypothesized that variants in the IL-17A gene would be associated with predisposition to the development of PE.…”