IL‐6 signaling pathway contributes to exercise pressor reflex in rats with femoral artery occlusion in association with Kv4 activity in muscle afferent nerves

Qin, Li; Qin, Lu; Li, Jianhua

doi:10.14814/phy2.14935

Cited by 2 publications

(1 citation statement)

References 75 publications

(90 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Both ipsilateral and contralateral sides of the L4‐L6 DRGs from the same rat were removed 24 h after the end of femoral artery ligation and 18 h after the end of I/R procedures (I/R group). β‐NGF levels (Bax et al., 1997 ) in the rat L4‐L6 DRGs were assayed with a modified ELISA (Li et al., 2021b ). This assay was performed at room temperature as instructed by the manufacturer (RayBiotech Co. Cat# ELR‐bNGF‐CL).…”

Section: Methodsmentioning

confidence: 99%

NGF contributes to activities of acid‐sensing ion channels in dorsal root ganglion neurons of male rats with experimental peripheral artery disease

Li,

2024

Physiological Reports

Self Cite

View full text Add to dashboard Cite

A feature of peripheral artery diseases (PAD) includes limb ischemia/reperfusion (I/R) and ischemia. Both I/R and ischemia amplify muscle afferent nerve‐activated reflex sympathetic nervous and blood pressure responses (termed as exercise pressor reflex). Nevertheless, the underlying mechanisms responsible for the exaggerated autonomic responses in PAD are undetermined. Previous studies suggest that acid‐sensing ion channels (ASICs) in muscle dorsal root ganglion (DRG) play a leading role in regulating the exercise pressor reflex in PAD. Thus, we determined if signaling pathways of nerve growth factor (NGF) contribute to the activities of ASICs in muscle DRG neurons of PAD. In particular, we examined ASIC1a and ASIC3 currents in isolectin B4‐negative muscle DRG neurons, a distinct subpopulation depending on NGF for survival. Hindlimb I/R and ischemia were obtained in male rats. In results, femoral artery occlusion increased the levels of NGF and NGF‐stimulated TrkA receptor in DRGs, whereas they led to upregulation of ASIC3 but not ASIC1a. In addition, application of NGF onto DRG neurons increased the density of ASIC3 currents and the effect of NGF was significantly attenuated by TrkA antagonist GW441756. Moreover, the enhancing effect of NGF on the density of ASIC3‐like currents was decreased by the respective inhibition of intracellular signaling pathways, namely JNK and NF‐κB, by antagonists SP600125 and PDTC. Our results suggest contribution of NGF to the activities of ASIC3 currents via JNK and NF‐κB signaling pathways in association with the exercise pressor reflex in experimental PAD.

show abstract

Section: Methodsmentioning

confidence: 99%