IL-6 inhibits IFN-γ induced autophagy in Mycobacterium tuberculosis H37Rv infected macrophages

Dutta, Rajesh Kumar; Kathania, Mahesh; Raje, Manoj; Majumdar, S.

doi:10.1016/j.biocel.2012.02.021

Cited by 88 publications

(85 citation statements)

References 32 publications

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“…Infection of pulmonary epithelial cells with MtB may indirectly lead to NF-kB activation, resulting in IL-1 release and activation of the classical NF-kB pathway (Bonizzi and Karin, 2004). IFN-γ induces autophagy in mycobacteria-infected cells, which is associated with protection against MtB (Dutta et al, 2012).…”

Section: Discussionmentioning

confidence: 99%

Diagnostic values of microRNA-31 in peripheral blood mononuclear cells for pediatric pulmonary tuberculosis in Chinese patients

Jx¹,

Xu²,

Han³

et al. 2015

Genet. Mol. Res.

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ABSTRACT. We investigated the diagnostic values of microRNA-31 in peripheral blood mononuclear cells (PBMCs) for pediatric pulmonary tuberculosis in Chinese patients. Sixty-five children with TB were selected for this study, which was conducted at the Department of Infectious Diseases People's Hospital of Laiwu City between December 2013 and December 2014. Sixty healthy children, selected in parallel, served as the control group. Real-time PCR was used to detect miR-31 expression in PBMCs. Serum levels of IL-6, TNF-α, NF-κB, and IFN-γ was detected by ELISA. ROC curve was employed to evaluate the diagnostic value of miR-31 in pediatric TB. Results show that expression of miRNA-31 in pediatric TB patients was significantly lower than that in normal children (0.48 ± 0.15 vs 1.23 ± 0.36, P < 0.05). By contrast, serum levels of the innate immune response cytokines, IL-6, TNF-α, NF-κB, and IFN-γ, were significantly higher in pediatric TB patients compared with normal children (P < 0.05). Furthermore, miRNA-31 expression was negatively correlated with serum levels of IL-6 (t = 69.91, P < 0.001), TNF-α (t = 10.96, P < 0.001), NF-κB (t = 39.94, P < 0.001), and IFN -γ (t = 37.94, P < 0.001). The cut-off threshold of miR-31 for pediatric TB diagnosis is 0.835 with a sensitivity of 98.5% and a (2015) specificity of 86.7%. Therefore, miR-31 has the potential to be a diagnostic marker in pediatric TB patients.

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Section: Discussionmentioning

confidence: 99%

Diagnostic values of microRNA-31 in peripheral blood mononuclear cells for pediatric pulmonary tuberculosis in Chinese patients

Jx¹,

Xu²,

Han³

et al. 2015

Genet. Mol. Res.

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“…Human macrophages were derived from blood monocytes as described earlier (Dutta et al, 2012). MDMs were infected with mycobacteria following the same method described for THP-1 cells as above.…”

Section: Infection Of Human Monocyte Derived Macrophages (Mdms)mentioning

confidence: 99%

“…Differentiated THP-1 cells were first infected with GFP-M. tuberculosis H37Rv as described above and then incubated in amino acids and serum deprived starvation medium (Hank's balanced salt solution, HBSS) (Dutta et al, 2012) in the presence or absence of IL-27 (50 ng/ml) for 4 h. Phagosome-lysosome fusion was further studied under confocal microscope after LysoTracker staining.…”

Section: Induction Of Phagosome-lysosome Fusion By Starvation In Mycomentioning

confidence: 99%

“…tuberculosis H37Rv was maintained and used as described previously (Dutta et al, 2012). Briefly, PMA (25 ng/ml) differentiated THP-1 cells were infected for 4 h with M. tuberculosis to give an infection ratio of 5-10 bacilli per macrophage as described previously (Dutta et al, 2012).…”

Section: Bacterial Culture and Infectionmentioning

confidence: 99%

“…Th2 cytokines IL-4 and IL-13 inhibit both IFN-␥ and starvation induced autophgy via activation of STAT-6 and mTOR (Harris et al, 2007). IL-6 and IL-10 also activate mTOR, inhibit phagosome maturation and as a result support the growth of intracellular MTB (Dutta et al, 2012;Park et al, 2011). IL-27, a hetero-dimeric cytokine composed of Epstein-Barr virus (EBV)-induced gene 3 (EBI3) and IL-27p28, exhibits both pro-and anti-inflammatory properties (Pflanz et al, 2002).…”

Section: Introductionmentioning

confidence: 97%

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IL-27 inhibits IFN-γ induced autophagy by concomitant induction of JAK/PI3 K/Akt/mTOR cascade and up-regulation of Mcl-1 in Mycobacterium tuberculosis H37Rv infected macrophages

Sharma

Dutta

Khan

et al. 2014

The International Journal of Biochemistry & Cell Biology

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Circular RNA TRAPPC6B inhibits intracellular Mycobacterium tuberculosis growth while inducing autophagy in macrophages by targeting microRNA‐874‐3p

Luo

Zhang

et al. 2021

Clin & Trans Imm

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Objectives Genetic and epigenetic mechanisms regulate antimicrobial immunity against Mycobacterium tuberculosis (Mtb) infection. Methods The present study assessed circular RNA TRAPPC6B (circTRAPPC6B) for antimicrobial immune functions and defined mechanisms wherein circTRAPPC6B regulates Mtb growth, autophagy and microRNA in macrophages. Results The Mtb infection of monocytes/macrophages resulted in a significantly decreased level of circTRAPPC6B that inhibited intracellular Mtb growth in macrophages. Conversely, circTRAPPC6B expression enhanced autophagy or autophagy‐associated protein LC3‐II production in Mtb‐infected macrophages. circTRAPPC6B‐enhanced autophagy aggregation or sequestration was also observed in fluorescence in situ hybridisation (FISH) analysis and confocal imaging. Mechanistically, circTRAPPC6B targets an inhibiting element miR‐874‐3p, as shown by bioinformatics, dual‐luciferase reporter gene analysis and pull‐down assay, respectively. Notably, miR‐874‐3p prohibited autophagy via suppressing autophagy protein ATG16L1 by binding to its 3′‐untranslated region (UTR) in Mtb‐infected macrophages and thus promoting intracellular Mtb growth. Concurrently, circTRAPPC6B enhanced autophagy in Mtb‐infected macrophages by blocking the ability of miR‐874‐3p to inhibit ATG16L1. Thus, circTRAPPC6B antagonises the ability of miR‐874‐3p to suppress ATG16L1 expression and activate and enhance autophagy sequestration to restrict Mtb growth in macrophages. Conclusion The current findings suggested that both circTRAPPC6B and miR‐874‐3p mechanisms can be explored as potential therapeutics against Mtb infection.

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IL-6 inhibits IFN-γ induced autophagy in Mycobacterium tuberculosis H37Rv infected macrophages

Cited by 88 publications

References 32 publications

Diagnostic values of microRNA-31 in peripheral blood mononuclear cells for pediatric pulmonary tuberculosis in Chinese patients

Diagnostic values of microRNA-31 in peripheral blood mononuclear cells for pediatric pulmonary tuberculosis in Chinese patients

IL-27 inhibits IFN-γ induced autophagy by concomitant induction of JAK/PI3 K/Akt/mTOR cascade and up-regulation of Mcl-1 in Mycobacterium tuberculosis H37Rv infected macrophages

Circular RNA TRAPPC6B inhibits intracellular Mycobacterium tuberculosis growth while inducing autophagy in macrophages by targeting microRNA‐874‐3p

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