IL-5 levels in the serum and blister fluid of patients with bullous pemphigoid: correlations with eosinophil cationic protein, RANTES, IgE and disease severity

D’Auria, L.; Pietravalle, M.; Mastroianni, Antonio; Ferraro, C.; Mussi, A.; Bonifati, C.; Giacalone, B; Ameglio, F.

doi:10.1007/s004030050272

Cited by 51 publications

(43 citation statements)

References 10 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…As regards other chronic bullous auto-immune diseases, high levels of IL-5 have been detected in BP blister fluid [23], correlated with both ECP levels and the number of blisters, as demonstrated by D’Auria et al [24]. On the other hand, this interleukin was detected in only half of the sera of 15 BP patients and at levels (median 6.8 pg/ml, range 1.6–112 pg/ml) significantly lower than those obtained from the blister fluid (median 326.5 pg/ml, range 76.2–665 pg/ml) [24].…”

Section: Discussionmentioning

confidence: 99%

“…On the other hand, this interleukin was detected in only half of the sera of 15 BP patients and at levels (median 6.8 pg/ml, range 1.6–112 pg/ml) significantly lower than those obtained from the blister fluid (median 326.5 pg/ml, range 76.2–665 pg/ml) [24]. These last findings seem to suggest that also in BP, as we hypothesize for DH, IL-5 exercises a prevalently local influence on eosinophil activation, since the relationship between its serum levels and the disease severity is not significant.…”

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Serum Eosinophil Cationic Protein, Myeloperoxidase, Tryptase, Eotaxin and Th2-Like Cytokines in Dermatitis herpetiformis

Caproni

Cardinali²,

A³

et al. 2002

Int Arch Allergy Immunol

View full text Add to dashboard Cite

Background: Dermatitis herpetiformis (DH) is a subepidermal bullous disease characterized by a neutrophilic and eosinophilic infiltrate, together with activated lymphocytes that show preferential polarization towards the Th2-like phenotype. To date, the role of neither soluble mediators, such as cytokines, nor serum levels of myeloperoxidase (MPO), eosinophil cationic protein (ECP) and tryptase (TRY) have been studied in the peripheral blood of DH. In this study we investigated 40 DH subjects for possible alterations in MPO, ECP, TRY, eotaxin, interleukin (IL) 4 and IL-5 concentrations to evaluate the role of neutrophils, eosinophils and mast cells in the induction of skin lesions. Sera from 20 healthy subjects were tested as controls. Methods: Eotaxin, IL-4 and IL-5 serum contents were determined by a solid-phase enzyme-linked immunosorbent assay. A double antibody radioimmunoassay was used to measure serum levels of MPO, ECP and TRY. Results: Normal levels of eotaxin and IL-4 were found in all DH subjects; increased IL-5 levels were found only in 1 subject. The MPO levels were significantly higher in DH subjects than in healthy controls (p < 0.01). There was also a significant difference between the serum ECP levels of DH and healthy controls (p < 0.05). TRY was not revealed in the serum of DH subjects. Conclusions: The determination of Th2-like cytokines and TRY serum levels is not important in quantifying the involvement of activated lymphocytes and mast cells in DH; however, the finding of high MPO and ECP serum levels seems to confirm that these products are good markers of the presence of activated neutrophils and eosinophils.

show abstract

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Serum Eosinophil Cationic Protein, Myeloperoxidase, Tryptase, Eotaxin and Th2-Like Cytokines in Dermatitis herpetiformis

Caproni

Cardinali²,

A³

et al. 2002

Int Arch Allergy Immunol

View full text Add to dashboard Cite

show abstract

“…Additionally, in many patients, the number of eosinophils in the peripheral blood is elevated [108]. It is noteworthy that high levels of IL-5, eotaxin, and eosinophilic cationic protein (ECP) have recently been detected in blister fluid of BP patients [109,110]. IL-5 promotes growth and activation of eosinophils.…”

Section: (3) Eosinophilsmentioning

confidence: 97%

The Pathophysiology of Bullous Pemphigoid

Kasperkiewicz

Zillikens

2007

Clinic Rev Allerg Immunol

157

155

View full text Add to dashboard Cite

Bullous pemphigoid (BP) is a blistering skin disease characterized by an autoimmune response to 2 hemidesmosomal proteins within the dermal-epidermal junction, designated BP180 and BP230. While BP230 localizes intracellularly and associates with the hemidesmosomal plaque, BP180 is a transmembrane glycoprotein with an extracellular domain. Most BP patients have autoantibodies binding to an immunodominant region of BP180, the noncollagenous 16A domain (NC16A), which is located extracellularly close to the transmembrane domain of the protein. Autoreactive T and B cell responses to BP180 have been found in patients with BP. Passive transfer of antibodies to the murine BP180 ectodomain triggers a blistering skin disease in mice that closely mimics human BP. Lesion formation in this animal model depends upon complement activation, mast cell degranulation and accumulation of neutrophils and eosinophils. Patients' autoantibodies to BP180 induce dermal-epidermal separation in cryosections of human skin when co-incubated with leukocytes. The loss of cell-matrix adhesion is mediated by proteinases released by granulocytes. The increased knowledge of the pathophysiology of BP should facilitate the development of novel therapeutic strategies for this disease.

show abstract

“…IL-5, the thymus and activation regulated chemokine (TARC), and eosinophil cationic protein levels. IL-5 and eosinophil cationic protein levels in blister fluid of BP patients were reported to correlate with total serum IgE levels, [65] and TARC levels in both sera and blister fluid were elevated in BP sera, and serum TARC levels correlated with total serum IgE levels. [66,67] These data in combination with the significantly higher serum levels of IL-4 and sCD30 [68] point toward a strong T-helpercell type 2 (Th2) response in BP.…”

Section: Elevated Total Serum Ige Levelsmentioning

confidence: 99%

IgE-mediated mechanisms in bullous pemphigoid and other autoimmune bullous diseases

Beek

Schulze

Zillikens

et al. 2015

Expert Review of Clinical Immunology

View full text Add to dashboard Cite

Autoimmune bullous diseases (AIBDs) are characterized by autoantibodies against structural proteins of the dermal-epidermal junction (in pemphigoid diseases) and the epidermal/ epithelial desmosomes (in pemphigus diseases). By far, the most common AIBD is bullous pemphigoid, which is immunopathologically characterized by autoantibodies against BP180 (type XVII collagen) and BP230. IgG and, to a lesser extent, IgA autoantibodies are the major autoantibody isotypes in these disorders. IgE autoantibodies are increasingly reported in particular in bullous pemphigoid. The development of specific and sensitive anti-BP180 IgE ELISA systems, the report of two experimental murine models employing IgE autoantibodies against BP180, and the successful treatment of bullous pemphigoid with the anti-IgE antibody omalizumab have raised interest in the role of IgE autoantibodies and the modulation of their production in AIBDs. Here, the relevance of IgE autoantibodies in the diagnosis, pathophysiology, and treatment decisions of AIBDs, with a focus on bullous pemphigoid, is reviewed. ARTICLE HISTORY

show abstract

IL-5 levels in the serum and blister fluid of patients with bullous pemphigoid: correlations with eosinophil cationic protein, RANTES, IgE and disease severity

Cited by 51 publications

References 10 publications

Serum Eosinophil Cationic Protein, Myeloperoxidase, Tryptase, Eotaxin and Th2-Like Cytokines in Dermatitis herpetiformis

Serum Eosinophil Cationic Protein, Myeloperoxidase, Tryptase, Eotaxin and Th2-Like Cytokines in Dermatitis herpetiformis

The Pathophysiology of Bullous Pemphigoid

IgE-mediated mechanisms in bullous pemphigoid and other autoimmune bullous diseases

Contact Info

Product

Resources

About